Overview

  • Product name

  • Description

    Rabbit polyclonal to SOCS7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human SOCS7 aa 6-165.
    Database link: O14512

  • Positive control

    • WB: MCF7, HeLa, Jurkat and U-87 MG cell lysate; rat heart and liver tissue lysate. ICC/IF: HepG2 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.03% Proclin
    Constituents: 40% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab224589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
WB 1/500 - 1/5000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).

Target

  • Function

    Regulates signaling cascades probably through protein ubiquitination and/or sequestration. Functions in insulin signaling and glucose homeostasis through IRS1 ubiquitination and subsequent proteasomal degradation. Inhibits also prolactin, growth hormone and leptin signaling by preventing STAT3 and STAT5 activation, sequestering them in the cytoplasm and reducing their binding to DNA. May be a substrate recognition component of a SCF-like E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
  • Tissue specificity

    Expressed in brain and leukocytes. Also in fetal lung fibroblasts and fetal brain.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • Domain

    The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes (By similarity). It is required for IRS1 ubiquitination and subsequent proteasomal degradation.
  • Cellular localization

    Cytoplasm. Cell membrane. Nucleus. Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Rapidly relocalizes to the nucleus after UV irradiation. Cytoplasmic location depends upon SEPT7 presence.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ash and phospholipase C gamma-binding protein antibody
    • NAP 4 antibody
    • NAP 4 Fragment antibody
    • NAP-4 antibody
    • NAP4 antibody
    • Nck antibody
    • Nck Ash and phospholipase C binding protein antibody
    • Nck Ash and phospholipase C gamma binding protein antibody
    • Nck associated protein 4 antibody
    • Nck-associated protein 4 antibody
    • NCKAP4 antibody
    • SH2 domain containing SOCS box protein antibody
    • SOCS 7 antibody
    • SOCS-7 antibody
    • SOCS4 antibody
    • SOCS6 antibody
    • Socs7 antibody
    • SOCS7_HUMAN antibody
    • Suppressor of cytokine signaling 7 antibody
    see all

Images

  • All lanes : Anti-SOCS7 antibody (ab224589) at 1/500 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 4 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cell lysate
    Lane 5 : Rat heart tissue lysate
    Lane 6 : Rat liver tissue lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 63 kDa
    Observed band size: 63 kDa

  • HepG2 (human liver hepatocellular carcinoma cell line) cells stained for SOCS7 (green) using ab224589 at 1/100 dilution in ICC/IF.

References

This product has been referenced in:

  • Su M  et al. miR-885-5p upregulation promotes colorectal cancer cell proliferation and migration by targeting suppressor of cytokine signaling. Oncol Lett 16:65-72 (2018). WB ; Human . Read more (PubMed: 29928388) »
See 1 Publication for this product

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