Key features and details
- Rabbit polyclonal to SOCS7
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-SOCS7 antibody
See all SOCS7 primary antibodies
DescriptionRabbit polyclonal to SOCS7
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Rat, Human
Recombinant fragment corresponding to Human SOCS7 aa 6-165.
Database link: O14512
- WB: MCF7, HeLa, Jurkat and U-87 MG cell lysate; rat heart and liver tissue lysate. ICC/IF: HepG2 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 40% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab224589 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/5000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).|
FunctionRegulates signaling cascades probably through protein ubiquitination and/or sequestration. Functions in insulin signaling and glucose homeostasis through IRS1 ubiquitination and subsequent proteasomal degradation. Inhibits also prolactin, growth hormone and leptin signaling by preventing STAT3 and STAT5 activation, sequestering them in the cytoplasm and reducing their binding to DNA. May be a substrate recognition component of a SCF-like E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Tissue specificityExpressed in brain and leukocytes. Also in fetal lung fibroblasts and fetal brain.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 SH2 domain.
Contains 1 SOCS box domain.
DomainThe SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes (By similarity). It is required for IRS1 ubiquitination and subsequent proteasomal degradation.
Cellular localizationCytoplasm. Cell membrane. Nucleus. Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Rapidly relocalizes to the nucleus after UV irradiation. Cytoplasmic location depends upon SEPT7 presence.
- Information by UniProt
- Ash and phospholipase C gamma-binding protein antibody
- NAP 4 antibody
- NAP 4 Fragment antibody
All lanes : Anti-SOCS7 antibody (ab224589) at 1/500 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 5 : Rat heart tissue lysate
Lane 6 : Rat liver tissue lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 63 kDa
Observed band size: 63 kDa
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for SOCS7 (green) using ab224589 at 1/100 dilution in ICC/IF.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224589 has been referenced in 1 publication.
- Su M et al. miR-885-5p upregulation promotes colorectal cancer cell proliferation and migration by targeting suppressor of cytokine signaling. Oncol Lett 16:65-72 (2018). WB ; Human . PubMed: 29928388