Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [NCI-R156-33] to SOD2/MnSOD (acetyl K122) - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Product nameAnti-SOD2/MnSOD (acetyl K122) antibody [NCI-R156-33] - BSA and Azide free
See all SOD2/MnSOD primary antibodies
DescriptionRabbit monoclonal [NCI-R156-33] to SOD2/MnSOD (acetyl K122) - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human SOD2/MnSOD aa 100-200 (acetyl K122) (Cysteine residue). The exact sequence is proprietary.
Database link: P04179
Ab240391 is the carrier-free version of ab214675. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab240391 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab240391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).|
FunctionDestroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
Involvement in diseaseGenetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
Sequence similaritiesBelongs to the iron/manganese superoxide dismutase family.
modificationsNitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- Indophenoloxidase B antibody
- IPO B antibody
- IPOB antibody
This data was developed using ab214675, the same antibody clone in a different buffer formulation.
Flag-SOD2/MnSOD (acetyl K122) was immunoprecipitated from HEK293T cells, transfected with Flag-tagged SOD2/MnSOD (acetyl K122) expression vector and treated with 1µM Trichostatin A (TSA) for 48 h, with anti-Flag antibody.
Western blot was performed from the immunoprecipitate using ab214675 at 1/200 dilution.
Horseradish peroxidase (HRP)-conjugated anti-Rabbit IgG, was used as secondary antibody at 1/10000 dilution.
Lane 1: IP samples washed and incubated with purified SIRT3 protein without NAD+, 10 µg.
Lane 2: IP samples washed and incubated with purified SIRT3 protein with NAD+ for two hours, 10 µg.
Lane 3: IP samples washed and incubated with purified SIRT3 protein without NAD+, then blocked with acetylated peptide (Ac-peptide), 10 µg.
Lane 4: IP samples washed and incubated with purified SIRT3 protein with NAD+ for two hours, then blocked with acetylated peptide (Ac-peptide), 10 µg.
Lane 5: IP samples washed and incubated with purified SIRT3 protein without NAD+, then blocked with non-acetylated control peptide (cont-peptide), 10 µg.
Lane 6: IP samples washed and incubated with purified SIRT3 protein with NAD+ for two hours, then blocked with non-acetylated control peptide (cont-peptide), 10 µg.
SIRT3 with NAD+ decreased SOD2/MnSOD acetylation in vitro. The expression profile observed is consistent with what has been described in the literature (PMID: 21172655; 25852572). This data was kindly provided by Dr. Gius (Northwestern University), and has been published in PMID: 21172655.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240391 has not yet been referenced specifically in any publications.