RecombinantRabMAb

Recombinant Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

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Western blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)
  • Western blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)
  • Western blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)
  • Western blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)
  • Western blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)
  • Dot Blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)
  • Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPVANR2] to SOD2/MnSOD (acetyl K68)
  • Suitable for: IHC-P, WB, Dot blot
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2]
    See all SOD2/MnSOD primary antibodies

  • Description

    Rabbit monoclonal [EPVANR2] to SOD2/MnSOD (acetyl K68)

  • Host species

    Rabbit

  • Specificity

    ab137037 only detects SOD2/MnSOD when acetylated at Lysine 68. According to BLAST analysis, the antibody might cross-react with Fer (Uniprot P70451) isoform 3 in mouse samples. No experiment has been done to confirm this possibility.

  • Tested applications

    Suitable for: IHC-P, WB, Dot blotmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human SOD2/MnSOD (acetyl K68). The exact sequence is proprietary.
    Database link: P04179
    (Peptide available as ab176149)

  • Positive control

    • WB: Trichostatin A treated SOD2 transfected HEK-293T lysates with non-acetyl peptide. Trichostatin A treated SOD2 transfected HEK-293T lysates. Mouse heart lysate. Rat kidney tissue lysate. SOD2 transfected HEK-293T lysate. IHC-P: Human cervical carcinoma. Human carcinoma kidney tissue. Rat liver tissue. Mouse kidney tissue. Human muscle tissue.

  • General notes

    This antibody was developed as part of a collaboration with the lab of David Guis at Vanderbilt University.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab137037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
WB 1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
Dot blot 1/1000.

Target

  • Function

    Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.

  • Involvement in disease

    Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.

  • Sequence similarities

    Belongs to the iron/manganese superoxide dismutase family.

  • Post-translational
    modifications

    Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.

  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Indophenoloxidase B antibody
    • IPO B antibody
    • IPOB antibody
    • Manganese containing superoxide dismutase antibody
    • Manganese SOD antibody
    • Manganese superoxide dismutase antibody
    • Mangano superoxide dismutase antibody
    • Mn SOD antibody
    • Mn superoxide dismutase antibody
    • MNSOD antibody
    • MVCD6 antibody
    • SOD 2 antibody
    • SOD2 antibody
    • SODM_HUMAN antibody
    • Superoxide dismutase [Mn] mitochondrial antibody
    • Superoxide dismutase [Mn], mitochondrial antibody
    • Superoxide dismutase 2 mitochondrial antibody
    see all

Images

  • Western blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)
    Western blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)
    All lanes : Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037) at 1/10000 dilution (purified)

    Lane 1 : Trichostatin A treated SOD2 transfected HEK-293T lysates with acetyl peptide
    Lane 2 : Trichostatin A treated SOD2 transfected HEK-293T lysates with non-acetyl peptide
    Lane 3 : Trichostatin A treated SOD2 transfected HEK-293T lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 24 kDa



    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human clear cell carcinoma kidney tissue labelling SOD2 (acetyl K68) with purified ab137037 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500).

    Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)
    Western blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)
    Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037) at 1/1000 dilution (purified) + Mouse heart lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 24 kDa



    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling SOD2 (acetyl K68) with purified ab137037 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500).

    Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)
    Western blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)
    Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037) at 1/1000 dilution (purified) + Rat kidney tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 24 kDa



    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling SOD2 (acetyl K68) with purified ab137037 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500).

    Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labelling SOD2 (acetyl K68) with unpurified ab137037 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)
    Western blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)
    All lanes : Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037) at 1/1000 dilution (unpurified)

    Lane 1 : Non transfected HEK-293T lysate
    Lane 2 : SOD2 transfected HEK-293T lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution

    Predicted band size: 24 kDa

  • Western blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)
    Western blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)
    All lanes : Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037) at 1/1000 dilution (unpurified)

    Lane 1 : Non transfected HEK-293T cell lysate with with Acetyl SOD2 (K68) peptide
    Lane 2 : SOD2 transfected HEK-293T cell lysate with control peptide

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution

    Predicted band size: 24 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling SOD2 (acetyl K68) with unpurified ab137037 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Dot Blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)
    Dot Blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

    Dot blot analysis labelling SOD2 (acetyl K68) with ab137037 at a dilution of 1/1000.  Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

    Blocking and diluting buffer: 5% NFDM/TBST.

    Lane 1: SOD2 (acetyl K68) acetylated peptide.

    Lane 2: SOD2 Non-acetylated peptide.

    Exposure time: 3 minutes.

  • Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)
    Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

References (41)

Publishing research using ab137037? Please let us know so that we can cite the reference in this datasheet.

ab137037 has been referenced in 41 publications.

  • Park JH  et al. Alpha-synuclein-induced mitochondrial dysfunction is mediated via a sirtuin 3-dependent pathway. Mol Neurodegener 15:5 (2020). PubMed: 31931835
  • Li Y  et al. A Newly Synthesized Rhamnoside Derivative Alleviates Alzheimer's Amyloid-ß-Induced Oxidative Stress, Mitochondrial Dysfunction, and Cell Senescence through Upregulating SIRT3. Oxid Med Cell Longev 2020:7698560 (2020). PubMed: 32104538
  • Kim YS  et al. Context-dependent activation of SIRT3 is necessary for anchorage-independent survival and metastasis of ovarian cancer cells. Oncogene 39:1619-1633 (2020). PubMed: 31723239
  • Li G  et al. a-Linolenic acid but not linolenic acid protects against hypertension: critical role of SIRT3 and autophagic flux. Cell Death Dis 11:83 (2020). PubMed: 32015327
  • Wu TT  et al. AKR1B1-Induced Epithelial-Mesenchymal Transition Mediated by RAGE-Oxidative Stress in Diabetic Cataract Lens. Antioxidants (Basel) 9:N/A (2020). PubMed: 32218152
View all Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Western blot abreview for Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Gel Running Conditions
Reduced Denaturing (10% SDS gel)
Loading amount
40 µg
Treatment
high fat diet for 2 months
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Dr. Sandra Sobocanec

Verified customer

Submitted Oct 12 2018

Western blot abreview for Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (macrophage cell line)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
40 µg
Specification
macrophage cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

Submitted Jun 08 2018

Western blot abreview for Anti-SOD2 (acetyl K68) antibody [EPVANR2]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (spinal cord dorsal horn)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
spinal cord dorsal horn
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Aug 08 2016

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