Name: Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2]
Brand: RabMAb
SKU: ab137037
Rating: 5 (3 reviews)

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Western blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPVANR2] to SOD2/MnSOD (acetyl K68)
Suitable for: IHC-P, WB, Dot blot
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2]
See all SOD2/MnSOD primary antibodies
Description

Rabbit monoclonal [EPVANR2] to SOD2/MnSOD (acetyl K68)
Host species

Rabbit
Specificity

ab137037 only detects SOD2/MnSOD when acetylated at Lysine 68. According to BLAST analysis, the antibody might cross-react with Fer (Uniprot P70451) isoform 3 in mouse samples. No experiment has been done to confirm this possibility.
Tested applications

Suitable for: IHC-P, WB, Dot blotmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human SOD2/MnSOD (acetyl K68). The exact sequence is proprietary.
Database link: P04179
(Peptide available as ab176149)
Positive control
- WB: Trichostatin A treated SOD2 transfected HEK-293T lysates with non-acetyl peptide. Trichostatin A treated SOD2 transfected HEK-293T lysates. Mouse heart lysate. Rat kidney tissue lysate. SOD2 transfected HEK-293T lysate. IHC-P: Human cervical carcinoma. Human carcinoma kidney tissue. Rat liver tissue. Mouse kidney tissue. Human muscle tissue.
General notes
This antibody was developed as part of a collaboration with the lab of David Guis at Vanderbilt University.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPVANR2
Isotype

IgG
Research areas

Alternative Versions
- Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] - BSA and Azide free (ab218529)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
WB	(3)	1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
Dot blot		1/1000.

Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
WB 1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
Dot blot 1/1000.

Function

Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
Involvement in disease

Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
Sequence similarities

Belongs to the iron/manganese superoxide dismutase family.
Post-translational
modifications

Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.
Cellular localization

Mitochondrion matrix.
Target information above from: UniProt accession P04179 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 6648 Human
- Entrez Gene: 20656 Mouse
- Entrez Gene: 24787 Rat
- Omim: 147460 Human
- SwissProt: P04179 Human
- SwissProt: P09671 Mouse
- SwissProt: P07895 Rat
- Unigene: 487046 Human
- Unigene: 290876 Mouse
- Unigene: 10488 Rat
see all
Alternative names
- Indophenoloxidase B antibody
- IPO B antibody
- IPOB antibody
- Manganese containing superoxide dismutase antibody
- Manganese SOD antibody
- Manganese superoxide dismutase antibody
- Mangano superoxide dismutase antibody
- Mn SOD antibody
- Mn superoxide dismutase antibody
- MNSOD antibody
- MVCD6 antibody
- SOD 2 antibody
- SOD2 antibody
- SODM_HUMAN antibody
- Superoxide dismutase [Mn] mitochondrial antibody
- Superoxide dismutase [Mn], mitochondrial antibody
- Superoxide dismutase 2 mitochondrial antibody
see all

Western blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

All lanes : Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037) at 1/10000 dilution (purified)

Lane 1 : Trichostatin A treated SOD2 transfected HEK-293T lysates with acetyl peptide
Lane 2 : Trichostatin A treated SOD2 transfected HEK-293T lysates with non-acetyl peptide
Lane 3 : Trichostatin A treated SOD2 transfected HEK-293T lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 24 kDa

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human clear cell carcinoma kidney tissue labelling SOD2 (acetyl K68) with purified ab137037 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500).

Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037) at 1/1000 dilution (purified) + Mouse heart lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

Predicted band size: 24 kDa

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling SOD2 (acetyl K68) with purified ab137037 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500).

Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037) at 1/1000 dilution (purified) + Rat kidney tissue lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

Predicted band size: 24 kDa

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling SOD2 (acetyl K68) with purified ab137037 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500).

Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labelling SOD2 (acetyl K68) with unpurified ab137037 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

All lanes : Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037) at 1/1000 dilution (unpurified)

Lane 1 : Non transfected HEK-293T lysate
Lane 2 : SOD2 transfected HEK-293T lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 24 kDa
Western blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

All lanes : Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037) at 1/1000 dilution (unpurified)

Lane 1 : Non transfected HEK-293T cell lysate with with Acetyl SOD2 (K68) peptide
Lane 2 : SOD2 transfected HEK-293T cell lysate with control peptide

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 24 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling SOD2 (acetyl K68) with unpurified ab137037 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Dot Blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

Dot blot analysis labelling SOD2 (acetyl K68) with ab137037 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

Blocking and diluting buffer: 5% NFDM/TBST.

Lane 1: SOD2 (acetyl K68) acetylated peptide.

Lane 2: SOD2 Non-acetylated peptide.

Exposure time: 3 minutes.
Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

Western blot protocols
Immunoprecipitation protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab137037? Please let us know so that we can cite the reference in this datasheet.

ab137037 has been referenced in 60 publications.

Wu L et al. Deficiency of ß-carotene oxygenase 2 induces mitochondrial fragmentation and activates the STING-IRF3 pathway in the mouse hypothalamus. J Nutr Biochem 88:108542 (2021). PubMed: 33129969
Zhang L et al. The anti-breast cancer property of physcion via oxidative stress-mediated mitochondrial apoptosis and immune response. Pharm Biol 59:303-310 (2021). PubMed: 33715588
Lv T et al. General Control of Amino Acid Synthesis 5-Like 1-Mediated Acetylation of Manganese Superoxide Dismutase Regulates Oxidative Stress in Diabetic Kidney Disease. Oxid Med Cell Longev 2021:6691226 (2021). PubMed: 33680286
Zhang S et al. Multi-omics approaches identify SF3B3 and SIRT3 as candidate autophagic regulators and druggable targets in invasive breast carcinoma. Acta Pharm Sin B 11:1227-1245 (2021). PubMed: 34094830
Zhang C et al. Melatonin Alleviates Contrast-Induced Acute Kidney Injury by Activation of Sirt3. Oxid Med Cell Longev 2021:6668887 (2021). PubMed: 34122726

View all Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Application

Western blot

Sample

Mouse Tissue lysate - whole (liver)

Gel Running Conditions

Reduced Denaturing (10% SDS gel)

Loading amount

40 µg

Treatment

high fat diet for 2 months

Specification

liver

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

DR. Sandra Sobocanec

Verified customer

Submitted Oct 12 2018

Application

Western blot

Sample

Mouse Cell lysate - whole cell (macrophage cell line)

Gel Running Conditions

Reduced Denaturing (4-20%)

Loading amount

40 µg

Specification

macrophage cell line

Blocking step

Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jun 08 2018

Application

Western blot

Sample

Rat Cell lysate - whole cell (spinal cord dorsal horn)

Gel Running Conditions

Reduced Denaturing

Loading amount

30 µg

Specification

spinal cord dorsal horn

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 08 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Applications

The Abpromise guarantee

Target

Function

Involvement in disease

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (60)

Customer reviews and Q&As

Western blot abreview for Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2]

Western blot abreview for Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2]

Western blot abreview for Anti-SOD2 (acetyl K68) antibody [EPVANR2]

Post-translational
modifications