Recombinant
RabMAb

Recombinant Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

Overview

  • Product name

    Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2]
    See all SOD2/MnSOD primary antibodies
  • Description

    Rabbit monoclonal [EPVANR2] to SOD2/MnSOD (acetyl K68)
  • Host species

    Rabbit
  • Specificity

    ab137037 only detects SOD2 when acetylated at Lysine 68. According to BLAST analysis, the antibody might cross-react with Fer (Uniprot P70451) isoform 3 in mouse samples. No experiment has been done to confirm this possibility.
  • Tested applications

    Suitable for: IP, IHC-P, WB, Dot blotmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SOD2/MnSOD (acetyl K68). The exact sequence is proprietary.
    Database link: P04179
    (Peptide available as ab176149)

  • Positive control

    • WB: Transfected 293T lysate. IHC-P: Human cervical carcinoma tissue and Human muscle tissue.
  • General notes

    This antibody was developed as part of a collaboration with the lab of David Guis at Vanderbilt University.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/30.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB 1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
Dot blot 1/1000.

Target

  • Function

    Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
  • Involvement in disease

    Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • Sequence similarities

    Belongs to the iron/manganese superoxide dismutase family.
  • Post-translational
    modifications

    Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • Indophenoloxidase B antibody
    • IPO B antibody
    • IPOB antibody
    • Manganese containing superoxide dismutase antibody
    • Manganese SOD antibody
    • Manganese superoxide dismutase antibody
    • Mangano superoxide dismutase antibody
    • Mn SOD antibody
    • Mn superoxide dismutase antibody
    • MNSOD antibody
    • MVCD6 antibody
    • SOD 2 antibody
    • SOD2 antibody
    • SODM_HUMAN antibody
    • Superoxide dismutase [Mn] mitochondrial antibody
    • Superoxide dismutase [Mn], mitochondrial antibody
    • Superoxide dismutase 2 mitochondrial antibody
    see all

Images

  • All lanes : Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037) at 1/10000 dilution (purified)

    Lane 1 : Trichostatin A treated SOD2 transfected 293T lysates with acetyl peptide
    Lane 2 : Trichostatin A treated SOD2 transfected 293T lysates with non-acetyl peptide
    Lane 3 : Trichostatin A treated SOD2 transfected 293T lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 24 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human clear cell carcinoma kidney tissue labelling SOD2 (acetyl K68) with purified ab137037 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
  • Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037) at 1/1000 dilution (purified) + Mouse heart lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 24 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling SOD2 (acetyl K68) with purified ab137037 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
  • Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037) at 1/1000 dilution (purified) + Rat kidney tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 24 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling SOD2 (acetyl K68) with purified ab137037 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cervical carcinoma tissue labelling SOD2 (acetyl K68) with unpurified ab137037 at 1/100 dilution.

  • All lanes : Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037) at 1/1000 dilution (unpurified)

    Lane 1 : Non transfected 293T lysate
    Lane 2 : SOD2 transfected 293T lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution

    Predicted band size: 24 kDa

  • All lanes : Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037) at 1/1000 dilution (unpurified)

    Lane 1 : Non transfected 293T cell lysate with with Acetyl SOD2 (K68) peptide
    Lane 2 : SOD2 transfected 293T cell lysate with control peptide

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution

    Predicted band size: 24 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human muscle tissue labelling SOD2 (acetyl K68) with unpurified ab137037 at 1/100 dilution.

  • Dot blot analysis of SOD2 (acetyl K68) acetylated peptide (Lane 1), SOD2 Non-acetylated peptide (Lane 2), labelling SOD2 (acetyl K68) with ab137037 at a dilution of 1/1000.  Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

References

This product has been referenced in:

  • Lee CF  et al. Targeting NAD+ Metabolism as Interventions for Mitochondrial Disease. Sci Rep 9:3073 (2019). Read more (PubMed: 30816177) »
  • Yi X  et al. SIRT3-Dependent Mitochondrial Dynamics Remodeling Contributes to Oxidative Stress-Induced Melanocyte Degeneration in Vitiligo. Theranostics 9:1614-1633 (2019). Read more (PubMed: 31037127) »
See all 21 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Gel Running Conditions
Reduced Denaturing (10% SDS gel)
Loading amount
40 µg
Treatment
high fat diet for 2 months
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Sandra Sobocanec

Verified customer

Submitted Oct 12 2018

Application
Western blot
Sample
Mouse Cell lysate - whole cell (macrophage cell line)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
40 µg
Specification
macrophage cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jun 08 2018

Application
Western blot
Sample
Rat Cell lysate - whole cell (spinal cord dorsal horn)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
spinal cord dorsal horn
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 08 2016

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