Recombinant
RabMAb

Recombinant Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] - BSA and Azide free (ab218529)

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Overview

  • Product name

    Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] - BSA and Azide free
    See all SOD2/MnSOD primary antibodies

  • Description

    Rabbit monoclonal [EPVANR2] to SOD2/MnSOD (acetyl K68) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Dot blot, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide (acetyl K68). The exact sequence is proprietary.
    Database link: P04179
    (Peptide available as ab176149)

  • Positive control

    • WB: Transfected 293T lysate. IHC-P: Human cervical carcinoma tissue and Human muscle tissue.

  • General notes

    ab218529 is the carrier-free version of ab137037 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab218529 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody was developed as part of a collaboration with the lab of David Guis at Vanderbilt University.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab218529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/1000.
WB Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Target

  • Function

    Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.

  • Involvement in disease

    Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.

  • Sequence similarities

    Belongs to the iron/manganese superoxide dismutase family.

  • Post-translational
    modifications

    Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.

  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Indophenoloxidase B antibody
    • IPO B antibody
    • IPOB antibody
    • Manganese containing superoxide dismutase antibody
    • Manganese SOD antibody
    • Manganese superoxide dismutase antibody
    • Mangano superoxide dismutase antibody
    • Mn SOD antibody
    • Mn superoxide dismutase antibody
    • MNSOD antibody
    • MVCD6 antibody
    • SOD 2 antibody
    • SOD2 antibody
    • SODM_HUMAN antibody
    • Superoxide dismutase [Mn] mitochondrial antibody
    • Superoxide dismutase [Mn], mitochondrial antibody
    • Superoxide dismutase 2 mitochondrial antibody
    see all

Images

  • Dot Blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] - BSA and Azide free (ab218529)
    Dot Blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] - BSA and Azide free (ab218529)

    Dot blot analysis of SOD2 (acetyl K68) acetylated peptide (Lane 1), SOD2 Non-acetylated peptide (Lane 2), labelling SOD2 (acetyl K68) with ab137037 at a dilution of 1/1000.  Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137037).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] - BSA and Azide free (ab218529)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] - BSA and Azide free (ab218529)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling SOD2 (acetyl K68) with purified ab137037 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137037).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] - BSA and Azide free (ab218529)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] - BSA and Azide free (ab218529)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling SOD2 (acetyl K68) with purified ab137037 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137037).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] - BSA and Azide free (ab218529)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] - BSA and Azide free (ab218529)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human clear cell carcinoma kidney tissue labelling SOD2 (acetyl K68) with purified ab137037 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137037).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] - BSA and Azide free (ab218529)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] - BSA and Azide free (ab218529)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cervical carcinoma tissue labelling SOD2 (acetyl K68) with unpurified ab137037 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137037).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] - BSA and Azide free (ab218529)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] - BSA and Azide free (ab218529)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human muscle tissue labelling SOD2 (acetyl K68) with unpurified ab137037 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137037).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Gurt I  et al. The Sirt1 Activators SRT2183 and SRT3025 Inhibit RANKL-Induced Osteoclastogenesis in Bone Marrow-Derived Macrophages and Down-Regulate Sirt3 in Sirt1 Null Cells. PLoS One 10:e0134391 (2015). Read more (PubMed: 26226624) »
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