Anti-SOD2/MnSOD antibody (ab118340)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-SOD2/MnSOD antibody
See all SOD2/MnSOD primary antibodies -
Description
Goat polyclonal to SOD2/MnSOD -
Host species
Goat -
Specificity
ab118340 is expected to recognize both reported isoforms (NP_000627.2; NP_001019637.1). The immunizing peptide represents the acetylation site including K122 according to isoform A. -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog, Pig, Zebrafish
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Immunogen
Synthetic peptide corresponding to Human SOD2/MnSOD aa 119-130 (internal sequence) (Cysteine residue).
Sequence:EAIKRDFGSFDK
Database link: P04179 -
Positive control
- Recombinant human SOD2/MnSOD protein (ab93946) can be used as a positive control in WB. Mouse and Rat spinal cord and Human Brain (cerebellum) lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab118340 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab118340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. Antibody detection limit dilution 1:64,000. |
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| WB | Use a concentration of 0.01 - 0.1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 25 kDa). |
Target
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Function
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. -
Involvement in disease
Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. -
Sequence similarities
Belongs to the iron/manganese superoxide dismutase family. -
Post-translational
modificationsNitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 6648 Human
- Entrez Gene: 20656 Mouse
- Entrez Gene: 24787 Rat
- Entrez Gene: 335799 Zebrafish
- Omim: 147460 Human
- SwissProt: P04179 Human
- SwissProt: P09671 Mouse
- SwissProt: P07895 Rat
see all -
Alternative names
- Indophenoloxidase B antibody
- IPO B antibody
- IPOB antibody
see all
Images
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Western blot - Anti-SOD2/MnSOD antibody (ab118340)All lanes : Anti-SOD2/MnSOD antibody (ab118340)
Lane 1 : Human Cerebellum lysate
Lane 2 : Mouse Brain lysate
Lane 3 : Rat Brain lysate
Lane 4 : Pig Brain lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 25 kDa -
Western blot - Anti-SOD2/MnSOD antibody (ab118340)All lanes : Anti-SOD2/MnSOD antibody (ab118340)
Lane 1 : HeLa cell lysate
Lane 2 : HepG2
Lane 3 : HEK293
Lane 4 : NIH3T3
Lysates/proteins at 35 µg per lane.
Predicted band size: 25 kDa -
Western blot - Anti-SOD2/MnSOD antibody (ab118340)Anti-SOD2/MnSOD antibody (ab118340) at 0.1 µg/ml (incubation time: 1 hour) + Mouse Spinal Cord lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 25 kDa
Protocols
Datasheets and documents
References
This product has been referenced in:
- Huang Y et al. Biological effects of pyrroloquinoline quinone on liver damage in Bmi-1 knockout mice. Exp Ther Med 10:451-458 (2015). Read more (PubMed: 26622336) »
