Overview

  • Product name
  • Description
    Rabbit polyclonal to SOD2/MnSOD
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, IHC-Fr, ICC/IF, IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Monkey, Gerbil
  • Immunogen

    Full length protein corresponding to Human SOD2/MnSOD.

  • Positive control
    • Rat brain tissue extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab13533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/150.
IHC-P Use at an assay dependent concentration.
WB 1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 26.6 kDa).
IP Use a concentration of 10 µg/ml.

Target

  • Function
    Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
  • Involvement in disease
    Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • Sequence similarities
    Belongs to the iron/manganese superoxide dismutase family.
  • Post-translational
    modifications
    Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Indophenoloxidase B antibody
    • IPO B antibody
    • IPOB antibody
    • Manganese containing superoxide dismutase antibody
    • Manganese SOD antibody
    • Manganese superoxide dismutase antibody
    • Mangano superoxide dismutase antibody
    • Mn SOD antibody
    • Mn superoxide dismutase antibody
    • MNSOD antibody
    • MVCD6 antibody
    • SOD 2 antibody
    • SOD2 antibody
    • SODM_HUMAN antibody
    • Superoxide dismutase [Mn] mitochondrial antibody
    • Superoxide dismutase [Mn], mitochondrial antibody
    • Superoxide dismutase 2 mitochondrial antibody
    see all

Images

  • All lanes : Anti-SOD2/MnSOD antibody (ab13533) at 1/5000 dilution

    Lane 1 : mouse liver whole cell lysate
    Lane 2 : mouse hepatocytes whole cell lysate

    Secondary
    All lanes : Goat anti-rabbit HRP conjugate at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 26.6 kDa
    Observed band size: 24 kDa
    why is the actual band size different from the predicted?


    Exposure time: 15 seconds

    See Abreview

  • ab13533 staining Superoxide Dismutase 2 in Human normal fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 1% BSA for 1 hour at 37°C. Samples were incubated with primary antibody (1/150 in 1% BSA) for 2 hours at 37°C. An undiluted Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody. Nuclei were counterstained with DAPI.

    See Abreview

  • ab13533 at 1/100 dilution staining Superoxide Dismutase in rat muscle fibre tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A goat anti-rabbit secondary was used at 1/10 dilution.

  • All lanes : Anti-SOD2/MnSOD antibody (ab13533) at 1/5000 dilution

    Lane 1 : 2ug mouse liver homogenate supernatant
    Lane 2 : 1ug mouse liver homogenate supernatant
    Lane 3 : 0.5ug mouse liver homogenate supernatant

    Secondary
    All lanes : HRP conjugated donkey anti-rabbit antibody

    Performed under reducing conditions.

    Predicted band size: 26.6 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds

    See Abreview

  • ab13533 staining Superoxide Dismutase 2 in human Hella cells by ICC/IF. Cells were acetone fixed and blocked in 5% serum for 1 hour at 24°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 24°C. The secondary antibody used was Alexa Fluor®594 donkey anti-rabbit diluted at 1/100.

    See Abreview

  • ab13533 staining NGF-differentiated PC12 cells (rat) by ICC/IF.  Cells were PFA fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 10% serum for 1 hour at 25°C.  The primary antibody was diluted to 2µg/ml in PBS and incubated with the sample for 1 hour at 25°C.  An Alexa Fluor® 488 conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

References

This product has been referenced in:
  • Cummins N  et al. Disease-associated tau impairs mitophagy by inhibiting Parkin translocation to mitochondria. EMBO J 38:N/A (2019). Read more (PubMed: 30538104) »
  • Chen HH  et al. Antihypertensive Potential of Coenzyme Q10 via Free Radical Scavenging and Enhanced Akt-nNOS Signaling in the Nucleus Tractus Solitarii in Rats. Mol Nutr Food Res 63:e1801042 (2019). Read more (PubMed: 30668894) »
See all 116 Publications for this product

Customer reviews and Q&As

11-20 of 36 Abreviews or Q&A

Application
Western blot
Loading amount
75 µg
Gel Running Conditions
Reduced Denaturing (12.5)
Sample
Rat Cell lysate - other (ovary)
Specification
ovary
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 11 2013

Answer

Thank you for contacting us and your interest in our products.
I am sorry for the confusion caused by the layout of the applications information. A duplicate entry for IHC-P has been made in error. Thank you for bringing this to our attention. This will be rectified.
The concentration recommendations are only a suggestion and will require optimisation in your particular experiment to obtain the best staining. I would suggest trying between 1-5 ug/mL to begin with and see if this requires further optimisation.
I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

Read More
Application
Western blot
Sample
Mouse Cell lysate - whole cell (liver)
Loading amount
5 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Aug 13 2012

Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacementcontaining 1 vialeach of ab38237,13533,38295.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Answer

Thank you for contacting Abcam.

We currently have 418 products that have cross reactivity records for Saccharomyces cerevisiaeon our catalog. While we try to test all of our antibodies in a wide range of species we cannot test each in every species. If the homologies of the immunogen used to create an antibody and the associated protein in a species are over 85% similar we do offer a testing discount program for researchers who wish to test an antibody in that untested species. More information about this 100% Abreview testing discount may be found at:www.abcam.com/collaborationdiscount


I did run alignments of the sequences for human SOD1 and SOD2 with the yeast homologs. Unfortunately, I found that these had only a 46% (SOD1) and 54% (SOD2) homology. BLASTs of each of the antibodies which were created from peptide fragments yielded no significant similarity. As such we could not guarantee than any of these products would successfully recognize SOD1 or SOD2 inSaccharomyces cerevisiae.

While these products do not qualify for our testing program, should you wish to test any product onSaccharomyces cerevisiae which has an 85% or greater homology with the immunogen please contact us as we will gladly create a testing discount code.

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

Read More

Question
Answer

Thank you for your email.

Since the antibody was raised against a full length protein and the epitope has not been mapped, we do not know where the antibody would bind the protein.

I am sorry for not having this information.

Please do not hesitate to contact us if you need any more advice or information.

Read More

Question
Answer

Thank you for contacting us.

The UniProt database entry for the human Superoxide Dismutase 2 is as follows:

http://www.uniprot.org/uniprot/P04179

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for contacting us.

While I feel that such testing would be valuable, unfortunately at this time we do not have a peptide testing discount program. We do have an abreview program in which you might post the results of such tests on the datasheet so that others may benefit from them. This program allows you to accrue abpoints which you may use towards products or an Amazon gift card.


I would like to thank you for the offer and wish you the best luck with your experiements. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for contacting Abcam and for sending me these questions.


In regards to your first question, we used an anti-SOD antibody for the western blot image. However the particular product we used is not offered by us for sale. We do offer a number of anti-SOD products amany are guaranteed to work in western, quite a few have been used in publications. Ab13533 and ab13534 each have 16 pubmed references, excellent abreviews and are guaranteed to work in pig tissues.


As to your second question regarding the activity of the peptide ab113179. The proteinhas not been tested for use in a cell-based assay; the activity assay listed was performed in vitro. We know it is active insomuch as it will convert hydrogen peroxide to O2 and water in an assay that can measure the rate of that conversion, but that is all. Ifyou wants to try treating cells with the protein the conditions for getting that to work would definitely need to be determined in the lab.


The Mn SOD protein would normally be found in mitochondria. If the protein did indeed go into cells,it could bedetected by Western with either an anti-SOD antibody or with an antibody against the his tag. However, if there was concern about separating the exogenous SOD from any that was there endogenously, detecting with an anti-His antibody would show specificity for SOD-200. Again I would recommend using ab13533 or ab13534 for western or ICCandI would definitely recommend a positive control.


As to which product, ab13533 or ab13534, you would chose to use for your staining. I feel that both are excellent choices. Both are highly reviewed and published. Ab13534 does have more IHC-P images and information available on the datasheet however, which may be valuable should have questions about procedures and staining patterns.


I hope this information has helped. Please let me know if you have any questions.

Read More
Application
Western blot
Sample
Rat Tissue lysate - whole (Isolated Airway)
Loading amount
20 µg
Specification
Isolated Airway
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Aug 30 2011

11-20 of 36 Abreviews or Q&A

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