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I am working on a project in yeast (Saccharomyces cerevisiae) and have need of antibodies targeting Sod1 and Sod2 for Western Blots. You have a number of antibodies for each target that are reactive with multiple species, but none mention yeast. Do you typically test yeast reactivity for you antibodies? Do you know of any customers that have had success with any of these antibodies in the context of yeast? I notice two polyclonal antibodies in particular that seem to have a wide range of species reactivity and might be good candidates: Anti-SOD2 antibody (ab13533) Anti-Superoxide Dismutase 1 antibody (ab13498) Thank you for your help,
Asked on May 01 2012
Thank you for contacting Abcam.
We currently have 418 products that have cross reactivity records for Saccharomyces cerevisiaeon our catalog. While we try to test all of our antibodies in a wide range of species we cannot test each in every species. If the homologies of the immunogen used to create an antibody and the associated protein in a species are over 85% similar we do offer a testing discount program for researchers who wish to test an antibody in that untested species. More information about this 100% Abreview testing discount may be found at:www.abcam.com/collaborationdiscount
I did run alignments of the sequences for human SOD1 and SOD2 with the yeast homologs. Unfortunately, I found that these had only a 46% (SOD1) and 54% (SOD2) homology. BLASTs of each of the antibodies which were created from peptide fragments yielded no significant similarity. As such we could not guarantee than any of these products would successfully recognize SOD1 or SOD2 inSaccharomyces cerevisiae.
While these products do not qualify for our testing program, should you wish to test any product onSaccharomyces cerevisiae which has an 85% or greater homology with the immunogen please contact us as we will gladly create a testing discount code.
I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.
Answered on May 01 2012