Immunocytochemistry/ Immunofluorescence abreview for Anti-SOD2 antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (NGF-differentiated PC12 cells)
Specification
NGF-differentiated PC12 cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton-X100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Other product details

Concentration
2 µg/ml
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS

Secondary antibody

Name
Non-Abcam antibody was used: goat anti-rabbit Alexa Fluor 488 (Molecular Probes
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
The cells were also stained with TOPRO-3 (Molecular Probes).
This anti-SOD2 antibody works as well as the polyclonal anti-SOD2 antibody, ab 13534

Dr. Anne Chiaramello

Verified customer

Submitted Jun 17 2008

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