Western blot abreview for Anti-SOD2 antibody

Good
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (Isolated Airway)
Loading amount
20 µg
Specification
Isolated Airway
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Other product details

Dilution
1/5000
Incubation time
1 hour(s) and 30 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: Sigma A9169
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/50000

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: ~23 kDa

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Submitted Aug 30 2011

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