Western blot abreview for Anti-SOD2 antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (liver)
Loading amount
5 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Dilution
1/2000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS

Secondary antibody

Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL+
Exposure
15 second(s)
Bands
Specific: 25 kDa

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Submitted Aug 13 2012

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