Western blot abreview for Anti-SOD2 antibody

Excellent
Abreviews
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% separating gel)
Sample
Rat Tissue lysate - whole (cerebellum)
Specification
cerebellum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
20 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% blocking milk in 0.1% TBST
Dilution
1/5000

Secondary antibody

Dilution
1/10000

Detection

Detection method
ECL
Bands
Specific: 25 kDa
Exposure
10 second(s)

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Submitted Feb 21 2014

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