Western blot abreview for Anti-SOD2 antibody

Excellent
Abreviews
Application
Western blot
Loading amount
80 µg
Gel Running Conditions
Reduced Denaturing (12.5% Acrylamide, 2D gel ph 3-10)
Sample
Pig Tissue lysate - other (Muscle Sarcoplasm)
Specification
Muscle Sarcoplasm
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-Tween
Dilution
1/5000

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL Prime
Bands
Specific: 25 kDa
Exposure
1 minute(s) and 0 second(s)

Dr. Shannon Cruzen

Verified customer

Submitted Sep 29 2014

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