Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SOD2 antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Rat gonad)
Antigen retrieval step
Enzymatic
Permeabilization
No
Specification
Rat gonad
Blocking step
Serum as blocking agent for 12 hour(s) and 45 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/200

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
Antigen retrieval: Protease/PBS (100mg/200ml) used for antigen retrieval H2O2/methanol solution (0.3%) used to destroy endogenous peroxidase activity AEC (Sigma#A6926) used as enzyme/chromagen.

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Submitted Apr 14 2015

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