Lane 1 and 3: Bone tissue (tibia) from 8w old Wistar male rats.
Lane 2: Bone tissue (tibia) from 17w old Wistar male rats treated with doxorubicin hydrochloride injections for 7w.
All bone tissue samples were collected and immediately stored at -80ºC until ready to process. They were then frozen in liquid nitrogen, pulverized in a electrical mill and homogenized (Omni Tissue Homogenizer) in RIPA buffer (1:1, v/w) with proteases inhibitor (1:20, v/w). The supernatant was collected after centrifuging for 5min at 3500g at 4ºC.
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Submitted Aug 25 2015
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