Western blot abreview for Anti-SOD2 antibody

Excellent
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (Bone tissue (Tibia))
Gel Running Conditions
Reduced Denaturing (12,5%)
Loading amount
50 µg
Specification
Bone tissue (Tibia)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBS-T
Dilution
1/2000

Secondary antibody

Dilution
1/2000
Name

Detection

Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 25 kDa Non-specific: 35 kDa
Positive control
Sample

Additional data

Additional Notes
Lane 1 and 3: Bone tissue (tibia) from 8w old Wistar male rats. Lane 2: Bone tissue (tibia) from 17w old Wistar male rats treated with doxorubicin hydrochloride injections for 7w. All bone tissue samples were collected and immediately stored at -80ºC until ready to process. They were then frozen in liquid nitrogen, pulverized in a electrical mill and homogenized (Omni Tissue Homogenizer) in RIPA buffer (1:1, v/w) with proteases inhibitor (1:20, v/w). The supernatant was collected after centrifuging for 5min at 3500g at 4ºC.

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Submitted Aug 25 2015

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