Western blot abreview for Anti-SOD2 antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Epithelial OVCA cells)
Gel Running Conditions
Reduced Denaturing (12.5%)
Loading amount
75 µg
Treatment
0- 100µM Perifosine 24 hr
Specification
Epithelial OVCA cells
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% milk
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL Western Blotting Substrate
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 22 kDa Non-specific: 34, 72 kDa

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Submitted Aug 09 2016

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