Western blot abreview for Anti-SOD2 antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (supernatant of liver homogenate)
Loading amount
2 µg
Specification
supernatant of liver homogenate
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5%

Other product details

Dilution
1/5000
Incubation time
3 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit secondary antibody
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
chemiluminescence
Exposure
10 second(s)
Bands
Specific: 25 kDa

Additional data

Additional Notes
PVDF membrane was used; sample was not treated with any of the protease inhibitors; unspecific bands were not observed; due to very strong intensity of the bands when the membrane was exposed to film longer than 1minute or when more than 5 micrograms of proteins were loaded per lane, we used only 2 micrograms of proteins in our samples, or even less.

Dr. Sandra Sobocanec

Verified customer

Submitted Jan 30 2007

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