Anti-SOD2/MnSOD antibody (ab13534)

Rabbit polyclonal SOD2/MnSOD antibody. Validated in WB, IP, ELISA, IHC, ICC/IF and tested in Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig and more.

Overview

  • Product name
  • Description
    Rabbit polyclonal to SOD2/MnSOD
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, ELISA, IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Drosophila melanogaster, Monkey
  • Immunogen

    Full length protein corresponding to Rat SOD2/MnSOD.

  • Positive control
    • ICC/IF: HeLa cells. IHC-P: Mouse back skin tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab13534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use a concentration of 2 µg/ml.
ELISA Use at an assay dependent concentration.
IHC-P 1/100.
WB 1/2000. Detects a band of approximately 25 kDa (predicted molecular weight: 26.6 kDa).
IP Use a concentration of 10 µg/ml.

Target

  • Function
    Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
  • Involvement in disease
    Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • Sequence similarities
    Belongs to the iron/manganese superoxide dismutase family.
  • Post-translational
    modifications
    Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Indophenoloxidase B antibody
    • IPO B antibody
    • IPOB antibody
    • Manganese containing superoxide dismutase antibody
    • Manganese SOD antibody
    • Manganese superoxide dismutase antibody
    • Mangano superoxide dismutase antibody
    • Mn SOD antibody
    • Mn superoxide dismutase antibody
    • MNSOD antibody
    • MVCD6 antibody
    • SOD 2 antibody
    • SOD2 antibody
    • SODM_HUMAN antibody
    • Superoxide dismutase [Mn] mitochondrial antibody
    • Superoxide dismutase [Mn], mitochondrial antibody
    • Superoxide dismutase 2 mitochondrial antibody
    see all

Images

  • Primary antibody was incubated for 16 hours at 4°C, at a 1/2000 dilution.Secondary antibody was a goat anti-rabbit conjugated to Alexa Fluor® 680, used at a 1/5000 dilution.

    See Abreview

  • ab13534 at 1/100 dilution staining Superoxide Dismutase 2 in mouse back skin tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A Fluorophore-conjugated goat anti-rabbit secondary was used at 1/50 dilution.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling SOD2/MnSOD with ab13534. Cells were fixed with 2% formaldehyde for 20 minutes at room temperature and incubated with the primary antibody at a dilution of 1/120 for 12 hours at 4°C. Followed by incubation with a R-PE-conjugated goat anti-rabbit IgG at a dilution of 1/200 for 2 hours at room temperature. Counterstained with DAPI (blue) at 1/40000 for 2 hours at room temperature. Magnification: 100x.

    Left: DAPI (blue) nuclear stain. Middle: SOD2/MnSOD. Right: Merge.

  • ab13534 staining SOD2 in Human colon and Horse Ileo tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/2000) for 18 hours.

    See Abreview

  • ab13534 staining differentiating (rat) PC12 cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.2% Triton X100 prior to blocking in 10% serum at RT for 1 hour.  The primary antibody was incubated with the sample for 1 hour at 2 µg/ml.  An Alexa Fluor® 488 conjugated goat anti-rabbit antibody diluted 1/1000 was used as the secondary.  Cells were counterstained with the nuclear TOPRO-3 stain.

    See Abreview

  • Lane 1: ab13534 + Mouse brain lysate (250µg)
    Lane 2: ab13534 + Mouse brain lysate (250µg, ½ dilution)
    Lane 3: Mouse brain lysate (15µg)
    Lane 4: Rat brain lysate (15µg)

    Ab13534 at 1:1000 immunoprecipitating SOD2 in mouse brain and rat brain lysate. For western blotting, donkey anti- rabbit IgG (HRP) was used as the secondary antibody.

  • All lanes : Anti-SOD2/MnSOD antibody (ab13534) at 1/2000 dilution

    Lane 1 : 40ug Fly Larvae
    Lane 2 : 40ug Fly Pre-Pupae
    Lane 3 : 40ug Fly Pupae
    Lane 4 : 40ug Fly Adult

    Secondary
    All lanes : HRP conjugated Donkey anti-rabbit IgG

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 26.6 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?
    Additional bands at: 14 kDa (possible non-specific binding), 45 kDa (possible non-specific binding), 70 kDa (possible non-specific binding)


    Exposure time: 10 seconds

    See Abreview

References

This product has been referenced in:
  • Liu L  et al. The antagonistic effect of tamoxifen against d-galactosamine/lipopolysaccharide-induced acute liver failure is associated with reactivation of hepatic nuclear factor-?B. Immunopharmacol Immunotoxicol N/A:1-7 (2019). Read more (PubMed: 30721100) »
  • Yamada M  et al. p62/SQSTM1 and Nrf2 are essential for exercise-mediated enhancement of antioxidant protein expression in oxidative muscle. FASEB J N/A:fj201900133R (2019). Read more (PubMed: 30913396) »
See all 54 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

1-10 of 14 Abreviews

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Mar 25 2019

Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Mar 20 2019

Application
Immunocytochemistry
Sample
Human Cultured Cells (HEK293)
Permeabilization
Yes - 0.02% Triton-X-100
Specification
HEK293
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 20 2019

Application
Immunocytochemistry
Sample
Rat Cultured Cells (Cardiomyocytes)
Permeabilization
Yes - 0.02% Triton-X-100
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Mar 19 2019

Application
Western blot
Sample
Rat Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Mar 19 2019

Application
Western blot
Sample
Monkey Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Mar 19 2019

Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Cardiomyocytes)
Permeabilization
Yes - 0.02% Triton-X-100
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 19 2019

Application
Western blot
Sample
Silk worm (Bombyx mori) Tissue lysate - whole (testis lysate (silkworm), BmN4 cell lysate (silkwo)
Gel Running Conditions
Non-reduced Denaturing (12% polyacrylamide)
Loading amount
10 µg
Specification
testis lysate (silkworm), BmN4 cell lysate (silkwo
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

陽水 野島

Verified customer

Submitted Aug 17 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (Ileo)
Specification
Ileo
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated

Abcam user community

Verified customer

Submitted Apr 12 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Liver)
Specification
Liver
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M Citrate pH6.0 110oC 2' Milestone RHS-2 microwave
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Oct 06 2009

1-10 of 14 Abreviews

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up