Product nameAnti-SOD2/MnSOD antibody [EPR2560Y]
See all SOD2/MnSOD primary antibodies
DescriptionRabbit monoclonal [EPR2560Y] to SOD2/MnSOD
Tested applicationsSuitable for: IHC-Fr, WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human SOD2/MnSOD aa 1-100 (N terminal). The exact sequence is proprietary.
- WB: SH-SY5Y, A549, HEK-293T and HAP1 cell lysates; Human heart tissue; Rat brain and heart lysate. IHC-P: Human liver tissue sections. IHC-Fr: Rat lung tissue sections.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab68155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 21906276|
|WB||1/1000 - 1/2000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionDestroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
Involvement in diseaseGenetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
Sequence similaritiesBelongs to the iron/manganese superoxide dismutase family.
modificationsNitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- Indophenoloxidase B antibody
- IPO B antibody
- IPOB antibody
Lane 1: SHSY5Y cell lysate (20 µg)
Lane 2: A549 cell lysate (20 µg)
Lane 3: Wild-type HEK-293T cell lysate (20 µg)
Lane 4: SOD2 knockout HEK-293T cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab68155 observed at 29 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab68155 was shown to react with SOD2/MnSOD in wild-type HEK-293T cells. Loss of signal was observed when knockout sample ab263835 was used. Wild-type and SOD2/MnSOD knockout samples were subjected to SDS-PAGE. ab68155 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: SOD2 knockout HAP1 cell lysate (20 µg)
Lane 3: Human heart tissue lysate (20 µg)
Lane 4: Rat heart tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab68155 observed at 29 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab68155 was shown to specifically react with SOD2 when SOD2 knockout samples were used. Wild-type and SOD2 knockout samples were subjected to SDS-PAGE. ab68155 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
All lanes : Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155) at 1/500 dilution
Lane 1 : Rat brain lysate
Lane 2 : SH-SY5Y lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
ab68155 at 1:100 dilution staining Superoxide Dismutase 2 in human liver tissue sections.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemical analysis of frozen rat lung tissue taken from rats with monocrotaline-exposure/pneumonectomy, staining SOD2 with ab68155.
This product has been referenced in:
- Chen C et al. Mitochondria and oxidative stress in ovarian endometriosis. Free Radic Biol Med 136:22-34 (2019). Read more (PubMed: 30926565) »
- Chen XF et al. Effect of puerarin in promoting fatty acid oxidation by increasing mitochondrial oxidative capacity and biogenesis in skeletal muscle in diabetic rats. Nutr Diabetes 8:1 (2018). Read more (PubMed: 29330446) »