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  1. Link

    sod2mnsod-antibody-mabcam-74231-ab74231.pdf

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Cell Biology Apoptosis Mitochondrial
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Validated using a knockout cell line

Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231)

  • Datasheet
Reviews (2) Submit a question References (1)

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Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231)
  • Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231)
  • Flow Cytometry - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231)
  • Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231)

Key features and details

  • Mouse monoclonal [mAbcam 74231] to SOD2/MnSOD
  • Suitable for: Flow Cyt, WB
  • Knockout validated
  • Reacts with: Rat, Human
  • Isotype: IgG1

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Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Knockout
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Human SOD2 (MnSOD) knockout HEK-293T cell line (ab255434)

View more associated products

Overview

  • Product name

    Anti-SOD2/MnSOD antibody [mAbcam 74231]
    See all SOD2/MnSOD primary antibodies
  • Description

    Mouse monoclonal [mAbcam 74231] to SOD2/MnSOD
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Zebrafish, Cynomolgus monkey, Macaque monkey, Orangutan
  • Immunogen

    Synthetic peptide within Human SOD2/MnSOD aa 50-150 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P04179

  • Positive control

    • WB: Recombinant human SOD2/MnSOD protein (ab93946), human liver; human brain; human heart; rat brain tissue lysates.
  • General notes

    This monoclonal antibody to SOD2 has been knockout validated in Western blot. The expected band for SOD2 was observed in wild type cells and the band was not seen in knockout cells.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    mAbcam 74231
  • Myeloma

    Sp2/0-Ag14
  • Isotype

    IgG1
  • Research areas

    • Cell Biology
    • Apoptosis
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Cardiovascular
    • Vasculature
    • Endothelium
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Antioxidants
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Metabolism
    • Types of disease
    • Cancer
    • Cancer
    • Cell Death
    • Apoptosis
    • Mitochondrial
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Positive Controls

    • Recombinant human SOD2/MnSOD protein (ab93946)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab74231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

WB (1)
Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 25 kDa).

We recommend using 3% milk as the blocking agent in Western Blot.

Notes
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

WB
Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 25 kDa).

We recommend using 3% milk as the blocking agent in Western Blot.

Target

  • Function

    Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
  • Involvement in disease

    Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • Sequence similarities

    Belongs to the iron/manganese superoxide dismutase family.
  • Post-translational
    modifications

    Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.
  • Cellular localization

    Mitochondrion matrix.
  • Target information above from: UniProt accession P04179 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6648 Human
    • Entrez Gene: 20656 Mouse
    • Entrez Gene: 24787 Rat
    • Entrez Gene: 335799 Zebrafish
    • Omim: 147460 Human
    • SwissProt: P04179 Human
    • SwissProt: P09671 Mouse
    • SwissProt: P07895 Rat
    • Unigene: 487046 Human
    • Unigene: 290876 Mouse
    • Unigene: 10488 Rat
    see all
  • Alternative names

    • Indophenoloxidase B antibody
    • IPO B antibody
    • IPOB antibody
    • Manganese containing superoxide dismutase antibody
    • Manganese SOD antibody
    • Manganese superoxide dismutase antibody
    • Mangano superoxide dismutase antibody
    • Mn SOD antibody
    • Mn superoxide dismutase antibody
    • MNSOD antibody
    • MVCD6 antibody
    • SOD 2 antibody
    • SOD2 antibody
    • SODM_HUMAN antibody
    • Superoxide dismutase [Mn] mitochondrial antibody
    • Superoxide dismutase [Mn], mitochondrial antibody
    • Superoxide dismutase 2 mitochondrial antibody
    see all

Images

  • Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231)
    Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231)

    Lane 1: Wild-type HAP1 cell lysate (40 µg)
    Lane 2: SOD2 knockout HAP1 cell lysate (40 µg)
    Lane 3: Human heart cell lysate (20 µg)
    Lane 4: Rat heart cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab74231 observed at 29 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab74231 was shown to specifically react with SOD2. Wild-type and SOD2 knockout samples were subjected to SDS-PAGE. ab74231 and ab181602 (loading control to GAPDH) were diluted 1 μg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231)
    Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231)
    All lanes : Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231) at 5 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human heart tissue lysate - total protein (ab29431)
    Lane 3 : Human brain tissue lysate - total protein (ab29466)
    Lane 4 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 25 kDa
    Observed band size: 23 kDa why is the actual band size different from the predicted?


    Exposure time: 5 minutes


    The Superoxide dismutase 2 protein has a predicted molecular weight of 25-kDa. However, the sequence contains a 24 residue transit peptide at the N-terminus and, when cleaved, the protein has an expected molecular weight of approximately 23-kDa.

     

    We recommend using 3% milk as the blocking agent in Western Blot.

  • Flow Cytometry - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231)
    Flow Cytometry - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231)
    Overlay histogram showing A549 cells stained with ab74231 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab74231, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231)
    Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231)
    Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231) at 1 µg/ml + Recombinant Human SOD2/MnSOD protein (ab82656) at 0.01 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 25 kDa


    Exposure time: 12 minutes

Protocols

  • Western blot protocols
  • Flow cytometry protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (1)

Publishing research using ab74231? Please let us know so that we can cite the reference in this datasheet.

ab74231 has been referenced in 1 publication.

  • Roos NJ  et al. Lapatinib Activates the Kelch-Like ECH-Associated Protein 1-Nuclear Factor Erythroid 2-Related Factor 2 Pathway in HepG2 Cells. Front Pharmacol 11:944 (2020). PubMed: 32694997

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-SOD2/MnSOD antibody [mAbcam 74231]

Poor
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (muscle)
Gel Running Conditions
Reduced Denaturing (12% acrylamide gel)
Loading amount
8 µg
Specification
muscle
Blocking step
Fish gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Read More

DR. Henriette Pilegaard

Verified customer

Submitted Feb 28 2019

Immunoprecipitation abreview for Anti-SOD2/MnSOD antibody [mAbcam 74231]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Mouse Tissue lysate - whole (Quadriceps muscle)
Total protein in input
100 µg
Immuno-precipitation step
Protein G
Specification
Quadriceps muscle
Read More

Abcam user community

Verified customer

Submitted Jan 05 2018

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