Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231)
![Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231)](https://www.abcam.com/ps/products/74/ab74231/Images/ab74231-263205-anti-sod2-mnsod-antibody-mabcam-74231-western-blot.jpg "Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231)")
Key features and details
- Mouse monoclonal [mAbcam 74231] to SOD2/MnSOD
- Suitable for: Flow Cyt, WB
- Knockout validated
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-SOD2/MnSOD antibody [mAbcam 74231]
See all SOD2/MnSOD primary antibodies -
Description
Mouse monoclonal [mAbcam 74231] to SOD2/MnSOD -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Zebrafish, Cynomolgus monkey, Macaque monkey, Orangutan
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Immunogen
Synthetic peptide within Human SOD2/MnSOD aa 50-150 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P04179 -
Positive control
- WB: Recombinant human SOD2/MnSOD protein (ab93946), human liver; human brain; human heart; rat brain tissue lysates.
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General notes
This monoclonal antibody to SOD2 has been knockout validated in Western blot. The expected band for SOD2 was observed in wild type cells and the band was not seen in knockout cells.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
mAbcam 74231 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74231 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 25 kDa).
We recommend using 3% milk as the blocking agent in Western Blot. |
| Notes |
|---|
|
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 25 kDa). We recommend using 3% milk as the blocking agent in Western Blot. |
Target
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Function
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. -
Involvement in disease
Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. -
Sequence similarities
Belongs to the iron/manganese superoxide dismutase family. -
Post-translational
modificationsNitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 6648 Human
- Entrez Gene: 20656 Mouse
- Entrez Gene: 24787 Rat
- Entrez Gene: 335799 Zebrafish
- Omim: 147460 Human
- SwissProt: P04179 Human
- SwissProt: P09671 Mouse
- SwissProt: P07895 Rat
see all -
Alternative names
- Indophenoloxidase B antibody
- IPO B antibody
- IPOB antibody
see all
Images
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Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231)
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: SOD2 knockout HAP1 cell lysate (40 µg)
Lane 3: Human heart cell lysate (20 µg)
Lane 4: Rat heart cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab74231 observed at 29 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab74231 was shown to specifically react with SOD2. Wild-type and SOD2 knockout samples were subjected to SDS-PAGE. ab74231 and ab181602 (loading control to GAPDH) were diluted 1 μg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231)](https://www.abcam.com/ps/products/74/ab74231/Images/ab74231-89237-anti-sod2-mnsod-antibody-mabcam-74231-western-blot.jpg)
Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231)All lanes : Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231) at 5 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lane 3 : Human brain tissue lysate - total protein (ab29466)
Lane 4 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutesThe Superoxide dismutase 2 protein has a predicted molecular weight of 25-kDa. However, the sequence contains a 24 residue transit peptide at the N-terminus and, when cleaved, the protein has an expected molecular weight of approximately 23-kDa.
We recommend using 3% milk as the blocking agent in Western Blot.
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![Flow Cytometry - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231)](https://www.abcam.com/ps/products/74/ab74231/Images/ab74231-89236-anti-sod2-mnsod-antibody-mabcam-74231-flow-cytometry.jpg)
Flow Cytometry - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231)Overlay histogram showing A549 cells stained with ab74231 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab74231, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. -
![Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231)](https://www.abcam.com/ps/products/74/ab74231/Images/ab74231-89235-anti-sod2-mnsod-antibody-mabcam-74231-western-blot.jpg)
Western blot - Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231)Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231) at 1 µg/ml + Recombinant Human SOD2/MnSOD protein (ab82656) at 0.01 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Exposure time: 12 minutes
Datasheets and documents
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Datasheet download
References (1)
ab74231 has been referenced in 1 publication.
- Roos NJ et al. Lapatinib Activates the Kelch-Like ECH-Associated Protein 1-Nuclear Factor Erythroid 2-Related Factor 2 Pathway in HepG2 Cells. Front Pharmacol 11:944 (2020). PubMed: 32694997
