Overview

  • Product name
  • Description
    Rabbit polyclonal to SODD
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human SODD aa 2-16.
    Sequence:

    SALRRSGYGPSDGPSC


    (Peptide available as ab8382)

  • Positive control
    • HeLa whole cell lysate or THP-1 whole cell lysate.
  • General notes

    SODDs.

     

    Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNF-R1 and Fas. Several novel death receptors including DR3, DR4, DR5, and DR6 were recently identified. Cell death signal is transduced by death domain containing adapter molecules through the interaction with death domain of these death receptors. A novel TNF-R1 interacting protein was recently identified and designated SODD for SODDs. SODD associates with the death domain of TNF-R1 and prevents constitutive activation of TNF-R1 signaling. TNF treatment releases SODD and permits adapter molecules such as TRADD recruiting to the active TNF-R1 complex, which activates TNF signaling pathways. SODD also interacts with DR3. SODD is ubiquitously expressed in human tissues and cell lines.

     This product was previously labelled as Silencer of Death Domain

     

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • Purity
    DEAE-Chromatography
  • Purification notes
    SODD Antibody is Antibody is DEAE purified.
  • Primary antibody notes
    Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNF-R1 and Fas. Several novel death receptors including DR3, DR4, DR5, and DR6 were recently identified. Cell death signal is transduced by death domain containing adapter molecules through the interaction with death domain of these death receptors. A novel TNF-R1 interacting protein was recently identifiedand designated SODD for silencer of death domains. SODD associates with the death domain of TNF-R1 and prevents constitutive activation of TNF-R1 signaling. TNF treatment releases SODD and permits adapter molecules such as TRADD recruiting to the active TNF-R1 complex, which activates TNF signaling pathways. SODD also interacts with DR3. SODD is ubiquitously expressed in human tissues and cell lines.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/2000. Detects a band of approximately 60 kDa.Can be blocked with SODD peptide (ab8382).

Target

Images

  • All lanes : Anti-SODD antibody (ab2048) at 1/500 dilution

    Lane 1 : HeLa whole cell lysate with absence of blocking peptide
    Lane 2 : THP-1 whole cell lysate with absence of blocking peptide
    Lane 3 : HeLa whole cell lysate with SODD peptide (ab8382)
    Lane 4 : THP-1 whole cell lysate with SODD peptide (ab8382)

    Observed band size: 60 kDa
    why is the actual band size different from the predicted?

  • ab2048 at 5µg/ml staining SODD in Hela cells by ICC/IF 

  • Immunofluorescence of SODD in Hela cells using ab2048 at 20 ug/ml.

References

This product has been referenced in:
  • Yi L  et al. Bcl-2 associated athanogene 4 promotes proliferation, migration and invasion of gastric cancer cells. Mol Med Rep 16:3753-3760 (2017). Read more (PubMed: 29067445) »
  • Boiani M  et al. The stress protein BAG3 stabilizes Mcl-1 protein and promotes survival of cancer cells and resistance to antagonist ABT-737. J Biol Chem 288:6980-90 (2013). WB ; Human . Read more (PubMed: 23341456) »
See all 2 Publications for this product

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