Overview

  • Product name

    Sodium 4-phenylbutyrate, Histone deacetylase inhibitor
  • Description

    Histone deacetylase inhibitor
  • Biological description

    Histone deacetylase (HDAC) inhibitor. Able to induce apoptosis, differentiation and promote the maturation of a variety of malignant cells. Inhibits glioma cell proliferation.
  • Purity

    > 99%
  • CAS Number

    1716-12-7
  • Chemical structure

    Chemical Structure

Properties

Applications

Our Abpromise guarantee covers the use of ab141253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab70362 staining adiponectin receptor 1 in HepG2 cells treated with sodium 4-phenylbutyrate (ab141253), by ICC/IF. Increase of adiponectin receptor 1 expression correlates with increased concentration of sodium 4-phenylbutyrate, as described in literature.
    The cells were incubated at 37°C for 6 hours in media containing different concentrations of ab141253 (sodium 4-phenylbutyrate) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab70362 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • The effect of sodium phenylbutyrate (NB) and sodium valproate (NV) on the expression of AHSP in K562 cells. 2D, two days of treatment; 4D, four days of treatment; 6D, six days of treatment.

References

This product has been referenced in:

  • Ammerpohl O  et al. Complementary effects of HDAC inhibitor 4-PB on gap junction communication and cellular export mechanisms support restoration of chemosensitivity of PDAC cells. Br J Cancer 96:73-81 (2007). Read more (PubMed: 17164759) »
  • Appelskog IB  et al. Histone deacetylase inhibitor 4-phenylbutyrate suppresses GAPDH mRNA expression in glioma cells. Int J Oncol 24:1419-25 (2004). Read more (PubMed: 15138583) »
  • Engelhard HH  et al. Inhibitory effects of phenylbutyrate on the proliferation, morphology, migration and invasiveness of malignant glioma cells. J Neurooncol 37:97-108 (1998). Read more (PubMed: 9524087) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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