Sodium 4-phenylbutyrate, Histone deacetylase inhibitor (ab141253)
Key features and details
- Histone deacetylase inhibitor
- CAS Number: 1716-12-7
- Purity: > 99%
- Soluble in water to 100 mM and in DMSO to 25 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Sodium 4-phenylbutyrate, Histone deacetylase inhibitor -
Description
Histone deacetylase inhibitor -
Biological description
Histone deacetylase (HDAC) inhibitor. Able to induce apoptosis, differentiation and promote the maturation of a variety of malignant cells. Inhibits glioma cell proliferation.
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Purity
> 99% -
CAS Number
1716-12-7 -
Chemical structure
Properties
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Chemical name
4-Phenylbutyric acid sodium salt -
Molecular weight
186.18 -
Molecular formula
C10H11NaO2 -
PubChem identifier
5258 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 100 mM and in DMSO to 25 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
O=C(CCCc1ccccc1)O[Na] -
Source
Synthetic
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Research areas
Associated products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab141253 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Functional Studies - Sodium 4-phenylbutyrate, Histone deacetylase inhibitor (ab141253)ab70362 staining adiponectin receptor 1 in HepG2 cells treated with sodium 4-phenylbutyrate (ab141253), by ICC/IF. Increase of adiponectin receptor 1 expression correlates with increased concentration of sodium 4-phenylbutyrate, as described in literature.
The cells were incubated at 37°C for 6 hours in media containing different concentrations of ab141253 (sodium 4-phenylbutyrate) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab70362 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. -
Cellular activation - Sodium 4-phenylbutyrate, Histone deacetylase inhibitor (ab141253)Image from Okhovat M A, et al. Plos One, 13(2), e0189267. Fig 1g,; doi: 10.1371/journal.pone.0189267The effect of sodium phenylbutyrate (NB) and sodium valproate (NV) on the expression of AHSP in K562 cells. 2D, two days of treatment; 4D, four days of treatment; 6D, six days of treatment.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (2)
ab141253 has been referenced in 2 publications.
- Okhovat MA et al. The effect of histone deacetylase inhibitors on AHSP expression. PLoS One 13:e0189267 (2018). PubMed: 29389946
- Qie X et al. Endoplasmic Reticulum Stress Mediates Methamphetamine-Induced Blood-Brain Barrier Damage. Front Pharmacol 8:639 (2017). PubMed: 28959203