Overview

  • Product name

    Sodium Assay Kit (Colorimetric)
  • Detection method

    Colorimetric
  • Sample type

    Saliva, Urine, Serum
  • Assay type

    Quantitative
  • Sensitivity

    25 µM
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Sodium Assay Kit (Colorimetric) (ab211096) provides a convenient two-step method to detect sodium ions (Na+) present in serum, urine and saliva. In this assay, sodium ions present in the sample are used by the enzyme β-galactosidase to produce an intermediate product, which reacts with a developer to generate a color signal that can be detect at OD = 405 nm.


    This kit can detect as low as 25 µM sodium in biological fluids. Endogenous mono-, di-, and trivalent ions, ascorbic acid, creatinine, glucose, urea, and bilirubin do not interfere with the assay.

  • Notes

    Sodium (Na+) is one of the most important electrolytes along with chloride, calcium and potassium. Sodium plays vital roles in the maintenance normal cell functions such as plasma volume, pH balance or transmission of nerve impulses. Healthy individuals can absorb sodium ingested in food, and kidneys maintain proper sodium balance by excreting its excess in urine. Normal Sodium intake has been defined to be between 200-500 mg/day. Hyponatremia (low sodium concentration in blood) can occur in patients with nephrotic syndrome, excessive vomiting and diarrhea, while Hypernatremia (high sodium concentration in blood) is developed in patients suffering from liver diseases, burns, and pregnancy. Traditionally, sodium concentration in clinical settings is determined by potentiometric, gravimetry, photometry, titrimetry and flame atomic emission spectroscopy, but these methods require expensive and complex protocols that need to be performed by trained personnel.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Na Assay Buffer 1 x 25ml
    Substrate 1 x 5ml
    β-Gal 1 x 15µl
    DTT (1 M) 1 x 0.25ml
    Na Developer 1 x 10ml
    Na Standard (1.5 M) 1 x 1ml

Images

  • Typical Sodium standard calibration curve.

  • Assay Specificity: Sodium, and other mono, di and trivalent cations (15 nmol/0.15 mM each) were tested to evaluate possible interferences. Interferences were found to be < 10% when data was normalized using Sodium as 100% activity. Blank: Assay buffer/DTT.

  • Estimation of sodium in human pooled serum off-the-clot (5 µL; 1:50 dilution) and human urine (10 µL; 1:100 dilution).

     

     

Protocols

References

This product has been referenced in:

  • Ballestas SA  et al. Improving hard palate wound healing using immune modulatory autotherapies. Acta Biomater 91:209-219 (2019). Read more (PubMed: 31029828) »
  • de Frutos S  et al. Chronic kidney disease induced by an adenine rich diet upregulates integrin linked kinase (ILK) and its depletion prevents the disease progression. Biochim Biophys Acta Mol Basis Dis 1865:1284-1297 (2019). Read more (PubMed: 30726718) »
See all 3 Publications for this product

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