Overview

  • Product name

    Anti-Sodium Iodide Symporter antibody
    See all Sodium Iodide Symporter primary antibodies
  • Description

    Rabbit polyclonal to Sodium Iodide Symporter
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Fr, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 468 - 517 (PSEQTMRVLP SSAARCVALS VNASGLLDPA LLPANDSSRA PSSGMDASRP) of Human Sodium Iodide Symporter (NP_000444)

  • Positive control

    • 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 69 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration. PubMed: 22719903
IHC-Fr Use at an assay dependent concentration. PubMed: 22719903
IHC-P Use at an assay dependent concentration. PubMed: 22719903

Target

  • Function

    Mediates iodide uptake in the thyroid gland.
  • Tissue specificity

    Expression is primarily in thyroid tissue, but also to a lower extent in mammary gland and ovary. Expression is reduced in tumors.
  • Involvement in disease

    Thyroid dyshormonogenesis 1
  • Sequence similarities

    Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Na(+)/I(-) cotransporter antibody
    • Na(+)/I(-) symporter antibody
    • Na+/I- cotransporter antibody
    • Na+/I-symporter antibody
    • NIS antibody
    • SC5A5_HUMAN antibody
    • Slc5a5 antibody
    • sodium iodide symporter antibody
    • Sodium-iodide symporter antibody
    • Sodium/iodide cotransporter antibody
    • Solute carrier family 5 (sodium iodide symporter) member 5 antibody
    • solute carrier family 5 (sodium/iodide cotransporter), member 5 antibody
    • Solute carrier family 5 member 5 antibody
    • TDH1 antibody
    see all

Images

  • Anti-Sodium Iodide Symporter antibody (ab83816) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 69 kDa
    Observed band size: 69 kDa

References

This product has been referenced in:

  • Meng Y  et al. In vitro study on human umbilical cord mesenchymal stem cells transfected with lentivirus-mediated hNIS-EGFP dual reporter gene and co-labeled with superparamagnetic iron oxide. Exp Ther Med 16:2208-2218 (2018). Read more (PubMed: 30186460) »
  • Zhang M  et al. Molecular In Vivo Imaging Using a Noninvasive Cardiac-Specific MLC-2v Promoter Driven Dual-Gene Recombinant Lentivirus Monitoring System. PLoS One 10:e0133952 (2015). IHC . Read more (PubMed: 26230833) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your enquiry.

I am sorry to confirm that discount codes for the Abtrial testing in new applications and species needs to be given out before the customer purchases. Therefore, we are regrettably not able to provide a code in this case.

We would be very pleased to receive feedback regarding the flow cytometry results from this antibody. I can suggest the customer may like to consider submitting an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact me again with any other questions.

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Answer

Thank you for your email. Apologies for the delay, but my colleague Miia is currently out of the office. Both ab17795 and ab83816 have been generated using peptides that would be unglycosylated, therefore they should recognize deglycosylated fragments. I hope this information helps.

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