Anti-Sodium Potassium ATPase antibody (ab353)

Submit a review Q&A (4)References (10)

Key features and details

  • Chicken polyclonal to Sodium Potassium ATPase
  • Suitable for: ELISA, IHC-Fr, ICC/IF, WB
  • Reacts with: Dog
  • Isotype: IgY

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab76020)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Sodium Potassium ATPase antibody
    See all Sodium Potassium ATPase primary antibodies

  • Description

    Chicken polyclonal to Sodium Potassium ATPase

  • Host species

    Chicken

  • Specificity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum as well as purified and partially purified ATPase, Sodium, Potassium [Canine Kidney].

  • Tested applications

    Suitable for: ELISA, IHC-Fr, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Dog

  • Immunogen

    Full length protein corresponding to Sodium Potassium ATPase. ATPase, Sodium, Potassium [Canine Kidney]

  • Positive control

    • ICC: MDCK cells ELISA: 1ug recombinant protein

  • General notes

    Centrifuge product if not completely clear after standing at room temperature. This product is for research use only and is not intended for therapeutic or diagnostic applications. Please note that we have 2 concentrations of this antibody: 1mg/ml and 10mg/ml. Please check the vial label.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Associated products

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.

This product has been assayed against 1.0 ug of ATPase, Sodium, Potassium in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Chicken IgG [H&L] (Rabbit)and ABTS (2,2'-azino-bis-[3-ethylbenthia-zoline-6-sulfonic acid])as a substrate for 30 minutes at room temperature.
IHC-Fr
Use at an assay dependent concentration.

(see review)
ICC/IF
Use at an assay dependent concentration.

(see review). Use amplification.
WB
Use at an assay dependent concentration.
Notes
ELISA
Use at an assay dependent concentration.

This product has been assayed against 1.0 ug of ATPase, Sodium, Potassium in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Chicken IgG [H&L] (Rabbit)and ABTS (2,2'-azino-bis-[3-ethylbenthia-zoline-6-sulfonic acid])as a substrate for 30 minutes at room temperature.
IHC-Fr
Use at an assay dependent concentration.

(see review)
ICC/IF
Use at an assay dependent concentration.

(see review). Use amplification.
WB
Use at an assay dependent concentration.

Target

  • Function

    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

  • Sequence similarities

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.

  • Post-translational
    modifications

    Phosphorylation on Tyr-10 modulates pumping activity.

  • Cellular localization

    Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Alternative names

    • ATPase Na+/K+ transporting alpha antibody
    • adenosinetriphosphatase antibody
    • AT1A1_HUMAN antibody
    • ATP1A1 antibody
    • ATP1A4 antibody
    • ATP1AL2 antibody
    • ATP1B antibody
    • ATP1B1 antibody
    • ATPase Na+/K+ transporting alpha 1 polypeptide antibody
    • ATPase Na+/K+ transporting alpha 4 polypeptide antibody
    • ATPase Na+/K+ transporting beta 1 polypeptide antibody
    • ATPase, Na+/K+ transporting, alpha polypeptide-like 2 antibody
    • ATPase, Na+/K+ transporting, beta 1 polypeptide antibody
    • Beta 1-subunit of Na(+),K(+)-ATPase antibody
    • Na(+)/K(+) ATPase alpha-1 subunit antibody
    • Na(+)/K(+) ATPase alpha-4 subunit antibody
    • Na+, K+ ATPase alpha subunit antibody
    • Na+/K+ ATPase 1 antibody
    • Na+/K+ ATPase 4 antibody
    • Na+/K+ ATPase, alpha-D polypeptide antibody
    • Na, K-ATPase beta-1 polypeptide antibody
    • Na, K-ATPase, alpha-A catalytic polypeptide antibody
    • Na,K-ATPase catalytic subunit alpha-A protein antibody
    • Na,K-ATPase subunit alpha-C antibody
    • polypeptide-like 2 antibody
    • Sodium pump 1 antibody
    • sodium pump 4 antibody
    • Sodium pump subunit alpha-1 antibody
    • sodium pump subunit alpha-4 antibody
    • sodium pump subunit beta-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-4 antibody
    • sodium-potassium ATPase subunit beta 1 (non-catalytic) antibody
    • sodium-potassium ATPase, alpha 4 polypeptide antibody
    • sodium-potassium-ATPase, alpha 1 polypeptide antibody
    • Sodium/potassium dependent ATPase beta 1 subunit antibody
    • Sodium/potassium transporting ATPase alpha 1 chain antibody
    • Sodium/potassium transporting ATPase beta 1 chain antibody
    • Sodium/potassium transporting ATPase subunit beta 1 antibody
    • sodium/potassium-dependent ATPase beta-1 subunit antibody
    • sodium/potassium-transporting ATPase alpha-4 chain antibody
    • sodium/potassium-transporting ATPase beta-1 chain antibody
    • Sodium/potassium-transporting ATPase subunit alpha-1 antibody
    • sodium/potassium-transporting ATPase subunit alpha-4 antibody
    see all

References (10)

Publishing research using ab353? Please let us know so that we can cite the reference in this datasheet.

ab353 has been referenced in 10 publications.

  • Zámbó B  et al. Clinically relevant mutations in the ABCG2 transporter uncovered by genetic analysis linked to erythrocyte membrane protein expression. Sci Rep 8:7487 (2018). ICC/IF . PubMed: 29749379
  • Martín-Gago P  et al. Covalent Protein Labeling at Glutamic Acids. Cell Chem Biol 24:589-597.e5 (2017). PubMed: 28434875
  • Varga K  et al. Histone deacetylase inhibitor- and PMA-induced upregulation of PMCA4b enhances Ca2+ clearance from MCF-7 breast cancer cells. Cell Calcium 55:78-92 (2014). PubMed: 24439526
  • Braune EB  et al. S/T phosphorylation of DLL1 is required for full ligand activity in vitro but dispensable for DLL1 function in vivo during embryonic patterning and marginal zone B cell development. Mol Cell Biol 34:1221-33 (2014). PubMed: 24449764
  • Liu R  et al. Itraconazole suppresses the growth of glioblastoma through induction of autophagy: involvement of abnormal cholesterol trafficking. Autophagy 10:1241-55 (2014). PubMed: 24905460
  • Antalffy G  et al. A C-terminal di-leucine motif controls plasma membrane expression of PMCA4b. Biochim Biophys Acta 1833:2561-72 (2013). ICC/IF . PubMed: 23830917
  • Czepiel M  et al. Differentiation of induced pluripotent stem cells into functional oligodendrocytes. Glia 59:882-92 (2011). PubMed: 21438010
  • Qin Y  et al. The mammalian Scribble polarity protein regulates epithelial cell adhesion and migration through E-cadherin. J Cell Biol 171:1061-71 (2005). ICC/IF ; Dog . PubMed: 16344308
  • Tauscher A & Kuver R ABCG5 and ABCG8 are expressed in gallbladder epithelial cells. Biochem Biophys Res Commun 307:1021-8 (2003). IF, IHC ; Dog . PubMed: 12878214
  • Lee J  et al. Polarized cholesterol and phospholipid efflux in cultured gall-bladder epithelial cells: evidence for an ABCA1-mediated pathway. Biochem J 364:475-84 (2002). PubMed: 12023891

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-4 of 4 Abreviews or Q&A

Question

ab35645: pas de marquage ICC/IF

Read More
Answer

Merci de nous avoir contactés.

Après avoir effectué une recherche pour un anticorps marqueur de la membrane plasmique qui pourrait convenir à vos besoins (autre qu'un anticorps de lapin ou IgG1 de souris),je n'ai trouvé que l'anti-Sodium Potassium ATPase référence https://www.abcam.com/ab353 dont nous avons brièvement parlé au téléphone la semaine dernière.

Cet anticorps polyclonal de poulet (IgY)n'a pour l'instant été testé que chez le chien. Cependant, l'alignement de séquences entre les Sodium/potassium-transporting ATPase subunit alpha-1 humaine et canine montre une homologie de 97% (voirfichier pdf ci-joint). Ceci suppose que l'anticorps ab353 pourrait fonctionner chez l'humain.

Désirez-vous tester cet anticorps en remplacement du ab35645 qui n'a pas fonctionné et pour lequel nous n'avons malheureusement pas d'autre lot que celui que vous avez reçu?

Dans l'attente de votre réponse je vous souhaite une très bonne journée.

Read More

Question

I am interesting in buying an antibody that is specific to Sodium potassium ATPase, that is specific to a peptide of this protein that lies within the cytoplasm. I believe that most commercial ATPase antibodies are specific to regions that are exposed on the cell surface. Do you know whether one of your antibodies actually target the internal face of the plasma membrane?

Read More
Answer

Thank you for your enquiry. The epitope has not been mapped in the cases of ab7671 and ab8344 therefore we do not know which part of the protein is recognised by those antibodies. As the immunogen used to generate ab353 is purified Na,K-ATPase from rabbit renal outermedulla and the antibody is a polyclonal antibody the product will recognise a number of epitopes on the protein, some are likely to be at the plasma membrane portion intracellularly and some extracellularly. I hope this information helps, please do not hesitate to contact us again if you need more information,

Read More

Question

The Na pump has 2 subunits, alpha and beta. Which of them is recognized by this ab? How many bands do you see in western blot?

Read More
Answer

This antibody was found to be reactive against the intact enzyme and enzyme subunits (alpha at 100 kDa and beta at 50 kDa) as well as glycosylated and non-glycosylated enzyme forms by western blot. The product is also reactive against 1.0ug of ATPase, Sodium, Potassium in a standard sandwich ELISA.

Read More

Question

Does the ab to canine Na-K-ATPase crossreact with the human or rodent enzyme?

Read More
Answer

Reactivity with other mammalian sources is unknown, but likely, due to the nature of the polyclonal antibody.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com