Anti-Sodium Potassium ATPase antibody (ab353)
Key features and details
- Chicken polyclonal to Sodium Potassium ATPase
- Suitable for: ELISA, IHC-Fr, ICC/IF, WB
- Reacts with: Dog
- Isotype: IgY
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Sodium Potassium ATPase antibody
See all Sodium Potassium ATPase primary antibodies -
Description
Chicken polyclonal to Sodium Potassium ATPase -
Host species
Chicken -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum as well as purified and partially purified ATPase, Sodium, Potassium [Canine Kidney]. -
Tested applications
Suitable for: ELISA, IHC-Fr, ICC/IF, WBmore details -
Species reactivity
Reacts with: Dog -
Immunogen
Full length protein corresponding to Sodium Potassium ATPase. ATPase, Sodium, Potassium [Canine Kidney]
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Positive control
- ICC: MDCK cells ELISA: 1ug recombinant protein
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General notes
Centrifuge product if not completely clear after standing at room temperature. This product is for research use only and is not intended for therapeutic or diagnostic applications. Please note that we have 2 concentrations of this antibody: 1mg/ml and 10mg/ml. Please check the vial label.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate, 0.878% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
This product has been assayed against 1.0 ug of ATPase, Sodium, Potassium in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Chicken IgG [H&L] (Rabbit)and ABTS (2,2'-azino-bis-[3-ethylbenthia-zoline-6-sulfonic acid])as a substrate for 30 minutes at room temperature. |
|
| IHC-Fr |
Use at an assay dependent concentration.
(see review) |
|
| ICC/IF |
Use at an assay dependent concentration.
(see review). Use amplification. |
|
| WB |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
ELISA
Use at an assay dependent concentration. This product has been assayed against 1.0 ug of ATPase, Sodium, Potassium in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Chicken IgG [H&L] (Rabbit)and ABTS (2,2'-azino-bis-[3-ethylbenthia-zoline-6-sulfonic acid])as a substrate for 30 minutes at room temperature. |
|
IHC-Fr
Use at an assay dependent concentration. (see review) |
|
ICC/IF
Use at an assay dependent concentration. (see review). Use amplification. |
|
WB
Use at an assay dependent concentration. |
Target
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Function
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. -
Sequence similarities
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. -
Post-translational
modificationsPhosphorylation on Tyr-10 modulates pumping activity. -
Cellular localization
Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Alternative names
- ATPase Na+/K+ transporting alpha antibody
- adenosinetriphosphatase antibody
- AT1A1_HUMAN antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab353 has been referenced in 10 publications.
- Zámbó B et al. Clinically relevant mutations in the ABCG2 transporter uncovered by genetic analysis linked to erythrocyte membrane protein expression. Sci Rep 8:7487 (2018). ICC/IF . PubMed: 29749379
- Martín-Gago P et al. Covalent Protein Labeling at Glutamic Acids. Cell Chem Biol 24:589-597.e5 (2017). PubMed: 28434875
- Varga K et al. Histone deacetylase inhibitor- and PMA-induced upregulation of PMCA4b enhances Ca2+ clearance from MCF-7 breast cancer cells. Cell Calcium 55:78-92 (2014). PubMed: 24439526
- Braune EB et al. S/T phosphorylation of DLL1 is required for full ligand activity in vitro but dispensable for DLL1 function in vivo during embryonic patterning and marginal zone B cell development. Mol Cell Biol 34:1221-33 (2014). PubMed: 24449764
- Liu R et al. Itraconazole suppresses the growth of glioblastoma through induction of autophagy: involvement of abnormal cholesterol trafficking. Autophagy 10:1241-55 (2014). PubMed: 24905460
- Antalffy G et al. A C-terminal di-leucine motif controls plasma membrane expression of PMCA4b. Biochim Biophys Acta 1833:2561-72 (2013). ICC/IF . PubMed: 23830917
- Czepiel M et al. Differentiation of induced pluripotent stem cells into functional oligodendrocytes. Glia 59:882-92 (2011). PubMed: 21438010
- Qin Y et al. The mammalian Scribble polarity protein regulates epithelial cell adhesion and migration through E-cadherin. J Cell Biol 171:1061-71 (2005). ICC/IF ; Dog . PubMed: 16344308
- Tauscher A & Kuver R ABCG5 and ABCG8 are expressed in gallbladder epithelial cells. Biochem Biophys Res Commun 307:1021-8 (2003). IF, IHC ; Dog . PubMed: 12878214
- Lee J et al. Polarized cholesterol and phospholipid efflux in cultured gall-bladder epithelial cells: evidence for an ABCA1-mediated pathway. Biochem J 364:475-84 (2002). PubMed: 12023891