Anti-Sodium Potassium ATPase antibody (ab353)

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Overview

  • Product name
    Anti-Sodium Potassium ATPase antibody
    See all Sodium Potassium ATPase primary antibodies
  • Description
    Chicken polyclonal to Sodium Potassium ATPase
  • Host species
    Chicken
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum as well as purified and partially purified ATPase, Sodium, Potassium [Canine Kidney].
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, ELISA, WBmore details
  • Species reactivity
    Reacts with: Dog
  • Immunogen

    ATPase, Sodium, Potassium [Canine Kidney]

  • Positive control
    • ICC: MDCK cells ELISA: 1ug recombinant protein
  • General notes
    Centrifuge product if not completely clear after standing at room temperature. This product is for research use only and is not intended for therapeutic or diagnostic applications. Please note that we have 2 concentrations of this antibody: 1mg/ml and 10mg/ml. Please check the vial label.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.

(see review). Use amplification.
IHC-Fr 1/100.

(see review)
ELISA 1/1000 - 1/6000.

This product has been assayed against 1.0 ug of ATPase, Sodium, Potassium in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Chicken IgG [H&L] (Rabbit)and ABTS (2,2’-azino-bis-[3-ethylbenthia-zoline-6-sulfonic acid])as a substrate for 30 minutes at room temperature.
WB 1/100 - 1/500.

Target

  • Function
    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
  • Sequence similarities
    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
  • Post-translational
    modifications
    Phosphorylation on Tyr-10 modulates pumping activity.
  • Cellular localization
    Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Alternative names
    • ATPase Na+/K+ transporting alpha antibody
    • adenosinetriphosphatase antibody
    • AT1A1_HUMAN antibody
    • ATP1A1 antibody
    • ATP1A4 antibody
    • ATP1AL2 antibody
    • ATP1B antibody
    • ATP1B1 antibody
    • ATPase Na+/K+ transporting alpha 1 polypeptide antibody
    • ATPase Na+/K+ transporting alpha 4 polypeptide antibody
    • ATPase Na+/K+ transporting beta 1 polypeptide antibody
    • ATPase, Na+/K+ transporting, alpha polypeptide-like 2 antibody
    • ATPase, Na+/K+ transporting, beta 1 polypeptide antibody
    • Beta 1-subunit of Na(+),K(+)-ATPase antibody
    • Na(+)/K(+) ATPase alpha-1 subunit antibody
    • Na(+)/K(+) ATPase alpha-4 subunit antibody
    • Na+, K+ ATPase alpha subunit antibody
    • Na+/K+ ATPase 1 antibody
    • Na+/K+ ATPase 4 antibody
    • Na+/K+ ATPase, alpha-D polypeptide antibody
    • Na, K-ATPase beta-1 polypeptide antibody
    • Na, K-ATPase, alpha-A catalytic polypeptide antibody
    • Na,K-ATPase catalytic subunit alpha-A protein antibody
    • Na,K-ATPase subunit alpha-C antibody
    • polypeptide-like 2 antibody
    • Sodium pump 1 antibody
    • sodium pump 4 antibody
    • Sodium pump subunit alpha-1 antibody
    • sodium pump subunit alpha-4 antibody
    • sodium pump subunit beta-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-4 antibody
    • sodium-potassium ATPase subunit beta 1 (non-catalytic) antibody
    • sodium-potassium ATPase, alpha 4 polypeptide antibody
    • sodium-potassium-ATPase, alpha 1 polypeptide antibody
    • Sodium/potassium transporting ATPase alpha 1 chain antibody
    • Sodium/potassium transporting ATPase subunit beta 1 antibody
    • sodium/potassium-dependent ATPase beta-1 subunit antibody
    • sodium/potassium-transporting ATPase alpha-4 chain antibody
    • sodium/potassium-transporting ATPase beta-1 chain antibody
    • Sodium/potassium-transporting ATPase subunit alpha-1 antibody
    • sodium/potassium-transporting ATPase subunit alpha-4 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Sodium Potassium ATPase antibody (ab353)
    Immunocytochemistry/ Immunofluorescence - Anti-Sodium Potassium ATPase antibody (ab353)Image from Qin Y et al, J Cell Biol. 2005 Dec 19;171(6):1061-71. Epub 2005 Dec 12, Fig 7. DOI 10.1083/jcb.200506094
    ab353 staining Sodium Potassium ATPase in Madin-Darby Canine Kidney (MDCK) cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in methanol/acetone (1:1) at -20°C for 10 minutes, permeabilized with 0.5% Triton X-100 in PBS, and blocked with 5% BSA in PBS for 1 hour before incubation with ab353 at a 1/250 dilution. An Alexa Fluor–conjugated secondary antibody was used at a dilution of 1/500 – 1/1000.

References

This product has been referenced in:
  • Zámbó B  et al. Clinically relevant mutations in the ABCG2 transporter uncovered by genetic analysis linked to erythrocyte membrane protein expression. Sci Rep 8:7487 (2018). ICC/IF . Read more (PubMed: 29749379) »
  • Martín-Gago P  et al. Covalent Protein Labeling at Glutamic Acids. Cell Chem Biol 24:589-597.e5 (2017). Read more (PubMed: 28434875) »
See all 9 Publications for this product

Publishing research using ab353? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Question

ab35645: pas de marquage ICC/IF

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Answer

Merci de nous avoir contactés.

Après avoir effectué une recherche pour un anticorps marqueur de la membrane plasmique qui pourrait convenir à vos besoins (autre qu'un anticorps de lapin ou IgG1 de souris),je n'ai trouvé que l'anti-Sodium Potassium ATPase référence https://www.abcam.com/ab353 dont nous avons brièvement parlé au téléphone la semaine dernière.

Cet anticorps polyclonal de poulet (IgY)n'a pour l'instant été testé que chez le chien. Cependant, l'alignement de séquences entre les Sodium/potassium-transporting ATPase subunit alpha-1 humaine et canine montre une homologie de 97% (voirfichier pdf ci-joint). Ceci suppose que l'anticorps ab353 pourrait fonctionner chez l'humain.

Désirez-vous tester cet anticorps en remplacement du ab35645 qui n'a pas fonctionné et pour lequel nous n'avons malheureusement pas d'autre lot que celui que vous avez reçu?

Dans l'attente de votre réponse je vous souhaite une très bonne journée.

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Question

I am interesting in buying an antibody that is specific to Sodium potassium ATPase, that is specific to a peptide of this protein that lies within the cytoplasm. I believe that most commercial ATPase antibodies are specific to regions that are exposed on the cell surface. Do you know whether one of your antibodies actually target the internal face of the plasma membrane?

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Answer

Thank you for your enquiry. The epitope has not been mapped in the cases of ab7671 and ab8344 therefore we do not know which part of the protein is recognised by those antibodies. As the immunogen used to generate ab353 is purified Na,K-ATPase from rabbit renal outermedulla and the antibody is a polyclonal antibody the product will recognise a number of epitopes on the protein, some are likely to be at the plasma membrane portion intracellularly and some extracellularly. I hope this information helps, please do not hesitate to contact us again if you need more information,

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Question

The Na pump has 2 subunits, alpha and beta. Which of them is recognized by this ab? How many bands do you see in western blot?

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Answer

This antibody was found to be reactive against the intact enzyme and enzyme subunits (alpha at 100 kDa and beta at 50 kDa) as well as glycosylated and non-glycosylated enzyme forms by western blot. The product is also reactive against 1.0ug of ATPase, Sodium, Potassium in a standard sandwich ELISA.

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Question

Does the ab to canine Na-K-ATPase crossreact with the human or rodent enzyme?

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Answer

Reactivity with other mammalian sources is unknown, but likely, due to the nature of the polyclonal antibody.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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