Overview

  • Product name
    Anti-Sodium Potassium ATPase antibody
    See all Sodium Potassium ATPase primary antibodies
  • Description
    Rabbit polyclonal to Sodium Potassium ATPase
  • Host species
    Rabbit
  • Specificity
    ab58475 detects endogenous levels of total Sodium Potassium ATPase protein.
  • Tested applications
    Suitable for: IHC-Fr, ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic non-phosphopeptide derived from human Sodium Potassium ATPase around the phosphorylation site of serine 16 (A-V-SP-E-H).

  • Positive control
    • Human brain tissue and HEK-293 cell extracts treated with PMA (125ng/ml, 30mins).

Properties

Applications

Our Abpromise guarantee covers the use of ab58475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 19923323
ELISA 1/5000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 113 kDa (predicted molecular weight: 113 kDa).

Target

  • Function
    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
  • Sequence similarities
    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
  • Post-translational
    modifications
    Phosphorylation on Tyr-10 modulates pumping activity.
  • Cellular localization
    Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATPase Na+/K+ transporting alpha antibody
    • adenosinetriphosphatase antibody
    • AT1A1_HUMAN antibody
    • ATP1A1 antibody
    • ATP1A4 antibody
    • ATP1AL2 antibody
    • ATP1B antibody
    • ATP1B1 antibody
    • ATPase Na+/K+ transporting alpha 1 polypeptide antibody
    • ATPase Na+/K+ transporting alpha 4 polypeptide antibody
    • ATPase Na+/K+ transporting beta 1 polypeptide antibody
    • ATPase, Na+/K+ transporting, alpha polypeptide-like 2 antibody
    • ATPase, Na+/K+ transporting, beta 1 polypeptide antibody
    • Beta 1-subunit of Na(+),K(+)-ATPase antibody
    • Na(+)/K(+) ATPase alpha-1 subunit antibody
    • Na(+)/K(+) ATPase alpha-4 subunit antibody
    • Na+, K+ ATPase alpha subunit antibody
    • Na+/K+ ATPase 1 antibody
    • Na+/K+ ATPase 4 antibody
    • Na+/K+ ATPase, alpha-D polypeptide antibody
    • Na, K-ATPase beta-1 polypeptide antibody
    • Na, K-ATPase, alpha-A catalytic polypeptide antibody
    • Na,K-ATPase catalytic subunit alpha-A protein antibody
    • Na,K-ATPase subunit alpha-C antibody
    • polypeptide-like 2 antibody
    • Sodium pump 1 antibody
    • sodium pump 4 antibody
    • Sodium pump subunit alpha-1 antibody
    • sodium pump subunit alpha-4 antibody
    • sodium pump subunit beta-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-4 antibody
    • sodium-potassium ATPase subunit beta 1 (non-catalytic) antibody
    • sodium-potassium ATPase, alpha 4 polypeptide antibody
    • sodium-potassium-ATPase, alpha 1 polypeptide antibody
    • Sodium/potassium transporting ATPase alpha 1 chain antibody
    • Sodium/potassium transporting ATPase subunit beta 1 antibody
    • sodium/potassium-dependent ATPase beta-1 subunit antibody
    • sodium/potassium-transporting ATPase alpha-4 chain antibody
    • sodium/potassium-transporting ATPase beta-1 chain antibody
    • Sodium/potassium-transporting ATPase subunit alpha-1 antibody
    • sodium/potassium-transporting ATPase subunit alpha-4 antibody
    see all

Images

  • All lanes : Anti-Sodium Potassium ATPase antibody (ab58475) at 1/500 dilution

    Lane 1 : 293 cell extracts treated with PMA (125ng/ml, 30mins)
    Lane 2 : 293 cell extracts treated with PMA (125ng/ml, 30mins) with immunising peptide

    Predicted band size: 113 kDa
    Observed band size: 113 kDa

  • ab58475 at 1/50 dilution staining Sodium Potassium ATPase in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

References

This product has been referenced in:
  • Hanson C  et al. Transplanting embryonic stem cells onto damaged human corneal endothelium. World J Stem Cells 9:127-132 (2017). Read more (PubMed: 28928909) »
  • Lovisa S  et al. Epithelial-to-mesenchymal transition induces cell cycle arrest and parenchymal damage in renal fibrosis. Nat Med 21:998-1009 (2015). IHC-P ; Mouse . Read more (PubMed: 26236991) »

See all 6 Publications for this product

Customer reviews and Q&As

The stock solution of the Optiblot DTT Reducer (10X) ab119199 in 2M. This means that once diluted 10 times this produces a 200 mM solution. Therefore if you usually use 10 mM with your samples I would suggest diluting this 200 times. The higher concent...

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Thank you for contacting us.   I have looked through our catalog of products searching for membrane marker that may perform better for you in adipose tissue. I concur with your assessment that an Anti-pan Cadherin antibody may be a better choice an...

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Thank you for your enquiry. This antibody will detect the alpha 1 subunit and not the beta 1 subunit. As far as we are aware, cross reactivity with monkey has not yet been tested for use with ab58475. All species cross-reactivity information is ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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