Overview

  • Product name

    Anti-Sodium Potassium ATPase antibody
    See all Sodium Potassium ATPase primary antibodies
  • Description

    Rabbit polyclonal to Sodium Potassium ATPase
  • Host species

    Rabbit
  • Specificity

    ab58475 detects endogenous levels of total Sodium Potassium ATPase protein.
  • Tested applications

    Suitable for: IHC-Fr, ELISA, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic non-phosphopeptide derived from human Sodium Potassium ATPase around the phosphorylation site of serine 16 (A-V-SP-E-H).

  • Positive control

    • Human brain tissue and HEK-293 cell extracts treated with PMA (125ng/ml, 30mins).

Properties

Applications

Our Abpromise guarantee covers the use of ab58475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 19923323
ELISA 1/5000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 113 kDa (predicted molecular weight: 113 kDa).

Target

  • Function

    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
  • Sequence similarities

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
  • Post-translational
    modifications

    Phosphorylation on Tyr-10 modulates pumping activity.
  • Cellular localization

    Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATPase Na+/K+ transporting alpha antibody
    • adenosinetriphosphatase antibody
    • AT1A1_HUMAN antibody
    • ATP1A1 antibody
    • ATP1A4 antibody
    • ATP1AL2 antibody
    • ATP1B antibody
    • ATP1B1 antibody
    • ATPase Na+/K+ transporting alpha 1 polypeptide antibody
    • ATPase Na+/K+ transporting alpha 4 polypeptide antibody
    • ATPase Na+/K+ transporting beta 1 polypeptide antibody
    • ATPase, Na+/K+ transporting, alpha polypeptide-like 2 antibody
    • ATPase, Na+/K+ transporting, beta 1 polypeptide antibody
    • Beta 1-subunit of Na(+),K(+)-ATPase antibody
    • Na(+)/K(+) ATPase alpha-1 subunit antibody
    • Na(+)/K(+) ATPase alpha-4 subunit antibody
    • Na+, K+ ATPase alpha subunit antibody
    • Na+/K+ ATPase 1 antibody
    • Na+/K+ ATPase 4 antibody
    • Na+/K+ ATPase, alpha-D polypeptide antibody
    • Na, K-ATPase beta-1 polypeptide antibody
    • Na, K-ATPase, alpha-A catalytic polypeptide antibody
    • Na,K-ATPase catalytic subunit alpha-A protein antibody
    • Na,K-ATPase subunit alpha-C antibody
    • polypeptide-like 2 antibody
    • Sodium pump 1 antibody
    • sodium pump 4 antibody
    • Sodium pump subunit alpha-1 antibody
    • sodium pump subunit alpha-4 antibody
    • sodium pump subunit beta-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-4 antibody
    • sodium-potassium ATPase subunit beta 1 (non-catalytic) antibody
    • sodium-potassium ATPase, alpha 4 polypeptide antibody
    • sodium-potassium-ATPase, alpha 1 polypeptide antibody
    • Sodium/potassium dependent ATPase beta 1 subunit antibody
    • Sodium/potassium transporting ATPase alpha 1 chain antibody
    • Sodium/potassium transporting ATPase beta 1 chain antibody
    • Sodium/potassium transporting ATPase subunit beta 1 antibody
    • sodium/potassium-dependent ATPase beta-1 subunit antibody
    • sodium/potassium-transporting ATPase alpha-4 chain antibody
    • sodium/potassium-transporting ATPase beta-1 chain antibody
    • Sodium/potassium-transporting ATPase subunit alpha-1 antibody
    • sodium/potassium-transporting ATPase subunit alpha-4 antibody
    see all

Images

  • All lanes : Anti-Sodium Potassium ATPase antibody (ab58475) at 1/500 dilution

    Lane 1 : 293 cell extracts treated with PMA (125ng/ml, 30mins)
    Lane 2 : 293 cell extracts treated with PMA (125ng/ml, 30mins) with immunising peptide

    Predicted band size: 113 kDa
    Observed band size: 113 kDa

  • ab58475 at 1/50 dilution staining Sodium Potassium ATPase in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

References

This product has been referenced in:

  • Yadav A  et al. Combination of medicinal honey and 904?nm superpulsed laser-mediated photobiomodulation promotes healing and impedes inflammation, pain in full-thickness burn. J Photochem Photobiol B 186:152-159 (2018). Read more (PubMed: 30048845) »
  • Gan L  et al. Functional analysis of the correlation between ABCB11 gene mutation and primary intrahepatic stone. Mol Med Rep N/A:N/A (2018). Read more (PubMed: 30431138) »
See all 12 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Flow Cytometry
Sample
Mouse Cell (trialed on kidney and spleen cells)
Permeabilization
No
Gating Strategy
renal tubule cells as gated by size and live B220+CD19+ cells (B cells)
Specification
trialed on kidney and spleen cells
Preparation
Cell harvesting/tissue preparation method: single cells suspension. Spleens were RBC lysed. Kidneys were digested with collagenase D and a Ficoll gradient was applied for isolation of lymphocyte populations
Sample buffer: PBS + 2% FCS + 1mM EDTA
Fixation
not fixed

Irene Chernova

Verified customer

Submitted Mar 07 2019

Question
Answer

The stock solution of the Optiblot DTT Reducer (10X) ab119199 in 2M. This means that once diluted 10 times this produces a 200 mM solution. Therefore if you usually use 10 mM with your samples I would suggest diluting this 200 times. The higher concentration should not really have an effect of the running of protein gels but as the pKa is around 8, in high concentration it can alter the pH somewhat which may affect the migration. We would normally suggest using 50 mM to ensure fully reducing the disulphide bonds in your samples.

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Answer

Thank you for contacting us.   I have looked through our catalog of products searching for membrane marker that may perform better for you in adipose tissue. I concur with your assessment that an Anti-pan Cadherin antibody may be a better choice and would recommend ab6529. Other markers that may interest you are ab115978 Anti-EMA and ab58285 Anti-Paralemmin. I have included the links to each of these products below. https://www.abcam.com/pan-Cadherin-antibody-Plasma-Membrane-Marker-ab6529.html https://www.abcam.com/EMA-antibody-EMA-201-ab115978.html https://www.abcam.com/Paralemmin-antibody-ab58285.html I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

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Answer

Thank you for your enquiry. This antibody will detect the alpha 1 subunit and not the beta 1 subunit. As far as we are aware, cross reactivity with monkey has not yet been tested for use with ab58475. All species cross-reactivity information is stated on Abcam product datasheets, and these are updated as soon as any new information is brought to our attention. If you decide to go ahead and purchase this product, we encourage you to submit an Abreview via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers. To find out more about our Abreview system, and how you can earn points for discounts on future purchases, please see the following URL: https://www.abcam.com/abreviews I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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