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Recombinant

Recombinant Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)

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Western blot - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)
  • Immunocytochemistry/ Immunofluorescence - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)
  • Flow Cytometry (Intracellular) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)
  • Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [EP1845Y] to Sodium Potassium ATPase - Chimeric – BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, Flow Cyt (Intra), WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free
    See all Sodium Potassium ATPase primary antibodies

  • Description

    Rat monoclonal [EP1845Y] to Sodium Potassium ATPase - Chimeric – BSA and Azide free

  • Host species

    Rat

  • Tested applications

    Suitable for: ICC/IF, IHC-P, Flow Cyt (Intra), WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • WB: HeLa, HeLa prepared from 1% SDS HOT lysis method, HeLa prepared from RIPA lysis method, RAW264.7 and C6 whole lysates. IHC-P: Human colon, Human hepatocellular carcinoma, Mouse kidney, Mouse stomach and Rat kidney, Rat stomach tissues. ICC: HeLa cells. Flow Cyt: HeLa cell.

  • General notes

    ab283341 is the carrier-free version of ab283345.

    This rat antibody has been engineered from a RabMAb parent antibody (ab76020). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab283341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 113 kDa.
Notes
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 113 kDa.

Target

  • Function

    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

  • Sequence similarities

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.

  • Post-translational
    modifications

    Phosphorylation on Tyr-10 modulates pumping activity.

  • Cellular localization

    Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ATPase Na+/K+ transporting alpha antibody
    • adenosinetriphosphatase antibody
    • AT1A1_HUMAN antibody
    • ATP1A1 antibody
    • ATP1A4 antibody
    • ATP1AL2 antibody
    • ATP1B antibody
    • ATP1B1 antibody
    • ATPase Na+/K+ transporting alpha 1 polypeptide antibody
    • ATPase Na+/K+ transporting alpha 4 polypeptide antibody
    • ATPase Na+/K+ transporting beta 1 polypeptide antibody
    • ATPase, Na+/K+ transporting, alpha polypeptide-like 2 antibody
    • ATPase, Na+/K+ transporting, beta 1 polypeptide antibody
    • Beta 1-subunit of Na(+),K(+)-ATPase antibody
    • Na(+)/K(+) ATPase alpha-1 subunit antibody
    • Na(+)/K(+) ATPase alpha-4 subunit antibody
    • Na+, K+ ATPase alpha subunit antibody
    • Na+/K+ ATPase 1 antibody
    • Na+/K+ ATPase 4 antibody
    • Na+/K+ ATPase, alpha-D polypeptide antibody
    • Na, K-ATPase beta-1 polypeptide antibody
    • Na, K-ATPase, alpha-A catalytic polypeptide antibody
    • Na,K-ATPase catalytic subunit alpha-A protein antibody
    • Na,K-ATPase subunit alpha-C antibody
    • polypeptide-like 2 antibody
    • Sodium pump 1 antibody
    • sodium pump 4 antibody
    • Sodium pump subunit alpha-1 antibody
    • sodium pump subunit alpha-4 antibody
    • sodium pump subunit beta-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-4 antibody
    • sodium-potassium ATPase subunit beta 1 (non-catalytic) antibody
    • sodium-potassium ATPase, alpha 4 polypeptide antibody
    • sodium-potassium-ATPase, alpha 1 polypeptide antibody
    • Sodium/potassium dependent ATPase beta 1 subunit antibody
    • Sodium/potassium transporting ATPase alpha 1 chain antibody
    • Sodium/potassium transporting ATPase beta 1 chain antibody
    • Sodium/potassium transporting ATPase subunit beta 1 antibody
    • sodium/potassium-dependent ATPase beta-1 subunit antibody
    • sodium/potassium-transporting ATPase alpha-4 chain antibody
    • sodium/potassium-transporting ATPase beta-1 chain antibody
    • Sodium/potassium-transporting ATPase subunit alpha-1 antibody
    • sodium/potassium-transporting ATPase subunit alpha-4 antibody
    see all

Images

  • Western blot - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)
    Western blot - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)
    All lanes : Anti-Sodium Potassium ATPase antibody [EP1845Y] - Rat IgG2a (ab283345) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate prepared from RIPA lysis method
    Lanes 2 & 4 : HeLa whole cell lysate prepared from 1% SDS HOT lysis method
    Lane 3 : HeLa whole cell lysate prepared from RIPA lysis method
    Lane 5 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 6 : RAW264.7 whole cell lysate
    Lane 7 : C6 (rat glial tumor glial cell), whole cell lysate
    Lane 8 : C6 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG (H+L), HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 113 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?



    This data was developed using ab283345, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Samples of Lanes 3, 4, 6, 8 are non-boiled as boiling may cause protein aggregates. 

    Exposure time: 1 second

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)

    This data was developed using ab283345 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Sodium Potassium ATPase with ab283345 at 1/5000 (0.222 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on human colon. The section was incubated with ab283345 for 10 mins at room temperature and followed by rat IgG antibody (ab102248) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)

    This data was developed using ab283345 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Sodium Potassium ATPase with ab283345 at 1/5000 (0.222 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on human hepatocellular carcinoma. The section was incubated with ab283345 for 10 mins at room temperature and followed by rat IgG antibody (ab102248) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)

    This data was developed using ab283345 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Sodium Potassium ATPase with ab283345 at 1/5000 (0.222 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Mainly membranous staining on mouse kidney. The section was incubated with ab283345 for 10 mins at room temperature and followed by rat IgG antibody (ab102248) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)

    This data was developed using ab283345 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Sodium Potassium ATPase with ab283345 at 1/5000 (0.222 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mouse stomach. The section was incubated with ab283345 for 10 mins at room temperature and followed by rat IgG antibody (ab102248) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)

    This data was developed using ab283345 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Sodium Potassium ATPase with ab283345 at 1/5000 (0.222 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Mainly membranous staining on rat kidney. The section was incubated with ab283345 for 10 mins at room temperature and followed by rat IgG antibody (ab102248) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)

    This data was developed using ab283345 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Sodium Potassium ATPase with ab283345 at 1/5000 (0.222 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on rat stomach. The section was incubated with ab283345 for 10 mins at room temperature and followed by rat IgG antibody (ab102248) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)
    Immunocytochemistry/ Immunofluorescence - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)

    This data was developed using ab283345 the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling Sodium Potassium ATPase with ab283345 at 1/50 (22.18 ug/ml) dilution, followed by ab150161 Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in HeLa cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150161 Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

     

  • Flow Cytometry (Intracellular) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)
    Flow Cytometry (Intracellular) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)

    This data was developed using ab283345 the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Sodium Potassium ATPase with ab283345 at 1/100 dilution (1ug) (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.

  • Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)
    Anti-Sodium Potassium ATPase antibody [EP1845Y] - Chimeric – BSA and Azide free (ab283341)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab283341? Please let us know so that we can cite the reference in this datasheet.

ab283341 has not yet been referenced specifically in any publications.

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