Flow Cytometry abreview for Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker (Alexa Fluor® 488)

Poor
Abreviews
Application
Flow Cytometry
Sample
Human Cell (macrophages (activated THP1))
Permeabilization
Yes - saponin
Gating Strategy
actually the application was Imagestream. Gated from singlets
Specification
macrophages (activated THP1)
Preparation
Cell harvesting/tissue preparation method: 3x10^6 THP1 cells pre 6 well were activated with PMA for 24 hours. Activated THP1 cells were dissociated with a dissociation buffer without enzymes and re-suspended in FACS buffer.
Sample buffer: FACS buffer contained 1% FBS in PBS
Fixation
Paraformaldehyde

Other product details

Incubation time
40 minute(s) · Temperature: 25°C · Diluent: FACS buffer
Dilution
1/100

Secondary antibody

Secondary antibody
None used

Additional data

Additional Notes
I hace tried different dilutions from 1:1000 to 1:50 and still the staining was poor even without fixation.
Abcam response
Abcam has not tested ab197713 in flow cytometry and thus we could not say how it should have performed in this application. This individual received a credit for testing the antibody in flow cytometry as part of our AbTrial program.

Dr. Lilach Abramovitz

Verified customer

Submitted Jun 17 2019

Sign up