actually the application was Imagestream. Gated from singlets
macrophages (activated THP1)
Cell harvesting/tissue preparation method: 3x10^6 THP1 cells pre 6 well were activated with PMA for 24 hours. Activated THP1 cells were dissociated with a dissociation buffer without enzymes and re-suspended in FACS buffer. Sample buffer: FACS buffer contained 1% FBS in PBS
I hace tried different dilutions from 1:1000 to 1:50 and still the staining was poor even without fixation.
Abcam has not tested ab197713 in flow cytometry and thus we could not say how it should have performed in this application. This individual received a credit for testing the antibody in flow cytometry as part of our AbTrial program.
Dr. Lilach Abramovitz
Submitted Jun 17 2019
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