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  1. Link

    sodium-potassium-atpase-antibody-plasma-membrane-marker-ab58475.pdf

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Tags & Cell Markers Subcellular Markers Plasma Membrane
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Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475)

  • Datasheet
  • SDS
Reviews (1)Q&A (3)References (19)

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Western blot - Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475)

Key features and details

  • Rabbit polyclonal to Sodium Potassium ATPase - Plasma Membrane Marker
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab76020)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker
    See all Sodium Potassium ATPase primary antibodies
  • Description

    Rabbit polyclonal to Sodium Potassium ATPase - Plasma Membrane Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic non-phosphopeptide derived from human Sodium Potassium ATPase around the phosphorylation site of serine 16 (A-V-SP-E-H).

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride

    Without Mg+2 and Ca+2
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Plasma Membrane
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • ATPases
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer
    • Isotype/Loading Controls
    • Loading Controls
    • NaKATPase

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab58475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/50 - 1/100.
WB
1/500 - 1/1000. Detects a band of approximately 113 kDa (predicted molecular weight: 113 kDa).
Notes
IHC-P
1/50 - 1/100.
WB
1/500 - 1/1000. Detects a band of approximately 113 kDa (predicted molecular weight: 113 kDa).

Target

  • Function

    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
  • Sequence similarities

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
  • Post-translational
    modifications

    Phosphorylation on Tyr-10 modulates pumping activity.
  • Cellular localization

    Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P05023 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 476 Human
    • Entrez Gene: 480 Human
    • Entrez Gene: 481 Human
    • Omim: 182310 Human
    • Omim: 182330 Human
    • Omim: 607321 Human
    • SwissProt: P05023 Human
    • SwissProt: P05026 Human
    • SwissProt: Q13733 Human
    • Unigene: 371889 Human
    see all
  • Alternative names

    • ATPase Na+/K+ transporting alpha antibody
    • adenosinetriphosphatase antibody
    • AT1A1_HUMAN antibody
    • ATP1A1 antibody
    • ATP1A4 antibody
    • ATP1AL2 antibody
    • ATP1B antibody
    • ATP1B1 antibody
    • ATPase Na+/K+ transporting alpha 1 polypeptide antibody
    • ATPase Na+/K+ transporting alpha 4 polypeptide antibody
    • ATPase Na+/K+ transporting beta 1 polypeptide antibody
    • ATPase, Na+/K+ transporting, alpha polypeptide-like 2 antibody
    • ATPase, Na+/K+ transporting, beta 1 polypeptide antibody
    • Beta 1-subunit of Na(+),K(+)-ATPase antibody
    • Na(+)/K(+) ATPase alpha-1 subunit antibody
    • Na(+)/K(+) ATPase alpha-4 subunit antibody
    • Na+, K+ ATPase alpha subunit antibody
    • Na+/K+ ATPase 1 antibody
    • Na+/K+ ATPase 4 antibody
    • Na+/K+ ATPase, alpha-D polypeptide antibody
    • Na, K-ATPase beta-1 polypeptide antibody
    • Na, K-ATPase, alpha-A catalytic polypeptide antibody
    • Na,K-ATPase catalytic subunit alpha-A protein antibody
    • Na,K-ATPase subunit alpha-C antibody
    • polypeptide-like 2 antibody
    • Sodium pump 1 antibody
    • sodium pump 4 antibody
    • Sodium pump subunit alpha-1 antibody
    • sodium pump subunit alpha-4 antibody
    • sodium pump subunit beta-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-4 antibody
    • sodium-potassium ATPase subunit beta 1 (non-catalytic) antibody
    • sodium-potassium ATPase, alpha 4 polypeptide antibody
    • sodium-potassium-ATPase, alpha 1 polypeptide antibody
    • Sodium/potassium dependent ATPase beta 1 subunit antibody
    • Sodium/potassium transporting ATPase alpha 1 chain antibody
    • Sodium/potassium transporting ATPase beta 1 chain antibody
    • Sodium/potassium transporting ATPase subunit beta 1 antibody
    • sodium/potassium-dependent ATPase beta-1 subunit antibody
    • sodium/potassium-transporting ATPase alpha-4 chain antibody
    • sodium/potassium-transporting ATPase beta-1 chain antibody
    • Sodium/potassium-transporting ATPase subunit alpha-1 antibody
    • sodium/potassium-transporting ATPase subunit alpha-4 antibody
    see all

Images

  • Western blot - Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475)
    Western blot - Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475)
    All lanes : Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475) at 1/500 dilution

    Lane 1 : 293 cell extracts treated with PMA (125ng/ml, 30mins)
    Lane 2 : 293 cell extracts treated with PMA (125ng/ml, 30mins) with immunising peptide

    Predicted band size: 113 kDa
    Observed band size: 113 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475)
    ab58475 at 1/50 dilution staining Sodium Potassium ATPase in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

Protocols

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (19)

Publishing research using ab58475? Please let us know so that we can cite the reference in this datasheet.

ab58475 has been referenced in 19 publications.

  • Li L & Zhang C Venlafaxine Attenuated the Cognitive and Memory Deficit in Mice Exposed to Isoflurane Alone. Front Neurol 12:591223 (2021). PubMed: 33708168
  • Keshri GK  et al. Effects of Pulsed 810?nm Al-Ga-As Diode Laser on Wound Healing Under Immunosuppression: A Molecular Insight. Lasers Surg Med 52:424-436 (2020). PubMed: 31483061
  • Sharanek A  et al. OSMR controls glioma stem cell respiration and confers resistance of glioblastoma to ionizing radiation. Nat Commun 11:4116 (2020). PubMed: 32807793
  • Hao RT  et al. NECTIN4 promotes papillary thyroid cancer cell proliferation, migration, and invasion and triggers EMT by activating AKT. Cancer Manag Res 11:2565-2578 (2019). PubMed: 31114323
  • Fischer RA  et al. Impairment of Membrane Repolarization Accompanies Axon Transport Deficits in Glaucoma. Front Neurosci 13:1139 (2019). PubMed: 31736686
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-4 of 4 Abreviews or Q&A

Flow Cytometry abreview for Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Flow Cytometry
Sample
Mouse Cell (trialed on kidney and spleen cells)
Permeabilization
No
Gating Strategy
renal tubule cells as gated by size and live B220+CD19+ cells (B cells)
Specification
trialed on kidney and spleen cells
Preparation
Cell harvesting/tissue preparation method: single cells suspension. Spleens were RBC lysed. Kidneys were digested with collagenase D and a Ficoll gradient was applied for isolation of lymphocyte populations
Sample buffer: PBS + 2% FCS + 1mM EDTA
Fixation
not fixed
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Irene Chernova

Verified customer

Submitted Mar 07 2019

Question

What is the concentration of the Optiblot DTT Reducer (10x) ab119199

Read More

Abcam community

Verified customer

Asked on Apr 17 2013

Answer

The stock solution of the Optiblot DTT Reducer (10X) ab119199 in 2M. This means that once diluted 10 times this produces a 200 mM solution. Therefore if you usually use 10 mM with your samples I would suggest diluting this 200 times. The higher concentration should not really have an effect of the running of protein gels but as the pKa is around 8, in high concentration it can alter the pH somewhat which may affect the migration. We would normally suggest using 50 mM to ensure fully reducing the disulphide bonds in your samples.

Read More

Abcam Scientific Support

Answered on Apr 17 2013

Question

This product did not perform well in adipose tissue, can you suggest a cell membrane marker that may work?

Read More

Abcam community

Verified customer

Asked on Dec 16 2011

Answer

Thank you for contacting us.   I have looked through our catalog of products searching for membrane marker that may perform better for you in adipose tissue. I concur with your assessment that an Anti-pan Cadherin antibody may be a better choice and would recommend ab6529. Other markers that may interest you are ab115978 Anti-EMA and ab58285 Anti-Paralemmin. I have included the links to each of these products below. https://www.abcam.com/pan-Cadherin-antibody-Plasma-Membrane-Marker-ab6529.html https://www.abcam.com/EMA-antibody-EMA-201-ab115978.html https://www.abcam.com/Paralemmin-antibody-ab58285.html I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

Read More

Abcam Scientific Support

Answered on Dec 16 2011

Question

I am interestend in purchasing the item sodium potassium ATPase antibody (ab58475) but if possible i would like to ask you some questions first: 1.Is this antibody specific for Na K ATPase alpha 1 subunit? 2.Does it react with Na K ATPase from monkey cells (Vero E6)? or it has not been tested yet Thank you very much in advance

Read More

Abcam community

Verified customer

Asked on Jan 28 2009

Answer

Thank you for your enquiry. This antibody will detect the alpha 1 subunit and not the beta 1 subunit. As far as we are aware, cross reactivity with monkey has not yet been tested for use with ab58475. All species cross-reactivity information is stated on Abcam product datasheets, and these are updated as soon as any new information is brought to our attention. If you decide to go ahead and purchase this product, we encourage you to submit an Abreview via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers. To find out more about our Abreview system, and how you can earn points for discounts on future purchases, please see the following URL: https://www.abcam.com/abreviews I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Jan 28 2009

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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