Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475)

Reviews (1)Q&A (3)References (15)
Western blot - Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475)

Key features and details

  • Rabbit polyclonal to Sodium Potassium ATPase - Plasma Membrane Marker
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker
    See all Sodium Potassium ATPase primary antibodies

  • Description

    Rabbit polyclonal to Sodium Potassium ATPase - Plasma Membrane Marker

  • Host species

    Rabbit

  • Specificity

    ab58475 detects endogenous levels of total Sodium Potassium ATPase protein.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic non-phosphopeptide derived from human Sodium Potassium ATPase around the phosphorylation site of serine 16 (A-V-SP-E-H).

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab58475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
IHC-P
1/50 - 1/100.
WB
1/500 - 1/1000. Detects a band of approximately 113 kDa (predicted molecular weight: 113 kDa).
Notes
IHC-P
1/50 - 1/100.
WB
1/500 - 1/1000. Detects a band of approximately 113 kDa (predicted molecular weight: 113 kDa).

Target

  • Function

    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

  • Sequence similarities

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.

  • Post-translational
    modifications

    Phosphorylation on Tyr-10 modulates pumping activity.

  • Cellular localization

    Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ATPase Na+/K+ transporting alpha antibody
    • adenosinetriphosphatase antibody
    • AT1A1_HUMAN antibody
    • ATP1A1 antibody
    • ATP1A4 antibody
    • ATP1AL2 antibody
    • ATP1B antibody
    • ATP1B1 antibody
    • ATPase Na+/K+ transporting alpha 1 polypeptide antibody
    • ATPase Na+/K+ transporting alpha 4 polypeptide antibody
    • ATPase Na+/K+ transporting beta 1 polypeptide antibody
    • ATPase, Na+/K+ transporting, alpha polypeptide-like 2 antibody
    • ATPase, Na+/K+ transporting, beta 1 polypeptide antibody
    • Beta 1-subunit of Na(+),K(+)-ATPase antibody
    • Na(+)/K(+) ATPase alpha-1 subunit antibody
    • Na(+)/K(+) ATPase alpha-4 subunit antibody
    • Na+, K+ ATPase alpha subunit antibody
    • Na+/K+ ATPase 1 antibody
    • Na+/K+ ATPase 4 antibody
    • Na+/K+ ATPase, alpha-D polypeptide antibody
    • Na, K-ATPase beta-1 polypeptide antibody
    • Na, K-ATPase, alpha-A catalytic polypeptide antibody
    • Na,K-ATPase catalytic subunit alpha-A protein antibody
    • Na,K-ATPase subunit alpha-C antibody
    • polypeptide-like 2 antibody
    • Sodium pump 1 antibody
    • sodium pump 4 antibody
    • Sodium pump subunit alpha-1 antibody
    • sodium pump subunit alpha-4 antibody
    • sodium pump subunit beta-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-4 antibody
    • sodium-potassium ATPase subunit beta 1 (non-catalytic) antibody
    • sodium-potassium ATPase, alpha 4 polypeptide antibody
    • sodium-potassium-ATPase, alpha 1 polypeptide antibody
    • Sodium/potassium dependent ATPase beta 1 subunit antibody
    • Sodium/potassium transporting ATPase alpha 1 chain antibody
    • Sodium/potassium transporting ATPase beta 1 chain antibody
    • Sodium/potassium transporting ATPase subunit beta 1 antibody
    • sodium/potassium-dependent ATPase beta-1 subunit antibody
    • sodium/potassium-transporting ATPase alpha-4 chain antibody
    • sodium/potassium-transporting ATPase beta-1 chain antibody
    • Sodium/potassium-transporting ATPase subunit alpha-1 antibody
    • sodium/potassium-transporting ATPase subunit alpha-4 antibody
    see all

Images

  • Western blot - Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475)
    Western blot - Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475)
    All lanes : Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475) at 1/500 dilution

    Lane 1 : 293 cell extracts treated with PMA (125ng/ml, 30mins)
    Lane 2 : 293 cell extracts treated with PMA (125ng/ml, 30mins) with immunising peptide

    Predicted band size: 113 kDa
    Observed band size: 113 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475)
    ab58475 at 1/50 dilution staining Sodium Potassium ATPase in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

References (15)

Publishing research using ab58475? Please let us know so that we can cite the reference in this datasheet.

ab58475 has been referenced in 15 publications.

  • Hao RT  et al. NECTIN4 promotes papillary thyroid cancer cell proliferation, migration, and invasion and triggers EMT by activating AKT. Cancer Manag Res 11:2565-2578 (2019). PubMed: 31114323
  • Fischer RA  et al. Impairment of Membrane Repolarization Accompanies Axon Transport Deficits in Glaucoma. Front Neurosci 13:1139 (2019). PubMed: 31736686
  • Xu E  et al. Asymmetric expression of GPR126 in the convex/concave side of the spine is associated with spinal skeletal malformation in adolescent idiopathic scoliosis population. Eur Spine J 28:1977-1986 (2019). PubMed: 31079250
  • Gan L  et al. Functional analysis of the correlation between ABCB11 gene mutation and primary intrahepatic stone. Mol Med Rep N/A:N/A (2018). PubMed: 30431138
  • Yadav A  et al. Combination of medicinal honey and 904?nm superpulsed laser-mediated photobiomodulation promotes healing and impedes inflammation, pain in full-thickness burn. J Photochem Photobiol B 186:152-159 (2018). PubMed: 30048845
View all Publications for this product

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Flow Cytometry abreview for Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker

 Excellent
Abreviews
abreview image
Application
Flow Cytometry
Sample
Mouse Cell (trialed on kidney and spleen cells)
Permeabilization
No
Gating Strategy
renal tubule cells as gated by size and live B220+CD19+ cells (B cells)
Specification
trialed on kidney and spleen cells
Preparation
Cell harvesting/tissue preparation method: single cells suspension. Spleens were RBC lysed. Kidneys were digested with collagenase D and a Ficoll gradient was applied for isolation of lymphocyte populations
Sample buffer: PBS + 2% FCS + 1mM EDTA
Fixation
not fixed
Read More

Irene Chernova

Verified customer

Submitted Mar 07 2019

Question

What is the concentration of the Optiblot DTT Reducer (10x) ab119199

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Answer

The stock solution of the Optiblot DTT Reducer (10X) ab119199 in 2M. This means that once diluted 10 times this produces a 200 mM solution. Therefore if you usually use 10 mM with your samples I would suggest diluting this 200 times. The higher concentration should not really have an effect of the running of protein gels but as the pKa is around 8, in high concentration it can alter the pH somewhat which may affect the migration. We would normally suggest using 50 mM to ensure fully reducing the disulphide bonds in your samples.

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Question

This product did not perform well in adipose tissue, can you suggest a cell membrane marker that may work?

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Answer

Thank you for contacting us.   I have looked through our catalog of products searching for membrane marker that may perform better for you in adipose tissue. I concur with your assessment that an Anti-pan Cadherin antibody may be a better choice and would recommend ab6529. Other markers that may interest you are ab115978 Anti-EMA and ab58285 Anti-Paralemmin. I have included the links to each of these products below. https://www.abcam.com/pan-Cadherin-antibody-Plasma-Membrane-Marker-ab6529.html https://www.abcam.com/EMA-antibody-EMA-201-ab115978.html https://www.abcam.com/Paralemmin-antibody-ab58285.html I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

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Question

I am interestend in purchasing the item sodium potassium ATPase antibody (ab58475) but if possible i would like to ask you some questions first: 1.Is this antibody specific for Na K ATPase alpha 1 subunit? 2.Does it react with Na K ATPase from monkey cells (Vero E6)? or it has not been tested yet Thank you very much in advance

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Answer

Thank you for your enquiry. This antibody will detect the alpha 1 subunit and not the beta 1 subunit. As far as we are aware, cross reactivity with monkey has not yet been tested for use with ab58475. All species cross-reactivity information is stated on Abcam product datasheets, and these are updated as soon as any new information is brought to our attention. If you decide to go ahead and purchase this product, we encourage you to submit an Abreview via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers. To find out more about our Abreview system, and how you can earn points for discounts on future purchases, please see the following URL: https://www.abcam.com/abreviews I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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