Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475)
Key features and details
- Rabbit polyclonal to Sodium Potassium ATPase - Plasma Membrane Marker
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker
See all Sodium Potassium ATPase primary antibodies -
Description
Rabbit polyclonal to Sodium Potassium ATPase - Plasma Membrane Marker -
Host species
Rabbit -
Specificity
ab58475 detects endogenous levels of total Sodium Potassium ATPase protein. -
Tested Applications & Species
Application Species IHC-P HumanWB Human -
Immunogen
Synthetic non-phosphopeptide derived from human Sodium Potassium ATPase around the phosphorylation site of serine 16 (A-V-SP-E-H).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab58475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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IHC-P |
Human
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WB |
Human
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Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/100.
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|
WB |
1/500 - 1/1000. Detects a band of approximately 113 kDa (predicted molecular weight: 113 kDa).
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Notes |
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IHC-P
1/50 - 1/100. |
WB
1/500 - 1/1000. Detects a band of approximately 113 kDa (predicted molecular weight: 113 kDa). |
Target
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Function
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. -
Sequence similarities
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. -
Post-translational
modificationsPhosphorylation on Tyr-10 modulates pumping activity. -
Cellular localization
Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 476 Human
- Entrez Gene: 480 Human
- Entrez Gene: 481 Human
- Omim: 182310 Human
- Omim: 182330 Human
- Omim: 607321 Human
- SwissProt: P05023 Human
- SwissProt: P05026 Human
see all -
Alternative names
- ATPase Na+/K+ transporting alpha antibody
- adenosinetriphosphatase antibody
- AT1A1_HUMAN antibody
see all
Images
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All lanes : Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475) at 1/500 dilution
Lane 1 : 293 cell extracts treated with PMA (125ng/ml, 30mins)
Lane 2 : 293 cell extracts treated with PMA (125ng/ml, 30mins) with immunising peptide
Predicted band size: 113 kDa
Observed band size: 113 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475)ab58475 at 1/50 dilution staining Sodium Potassium ATPase in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab58475 has been referenced in 15 publications.
- Hao RT et al. NECTIN4 promotes papillary thyroid cancer cell proliferation, migration, and invasion and triggers EMT by activating AKT. Cancer Manag Res 11:2565-2578 (2019). PubMed: 31114323
- Fischer RA et al. Impairment of Membrane Repolarization Accompanies Axon Transport Deficits in Glaucoma. Front Neurosci 13:1139 (2019). PubMed: 31736686
- Xu E et al. Asymmetric expression of GPR126 in the convex/concave side of the spine is associated with spinal skeletal malformation in adolescent idiopathic scoliosis population. Eur Spine J 28:1977-1986 (2019). PubMed: 31079250
- Gan L et al. Functional analysis of the correlation between ABCB11 gene mutation and primary intrahepatic stone. Mol Med Rep N/A:N/A (2018). PubMed: 30431138
- Yadav A et al. Combination of medicinal honey and 904?nm superpulsed laser-mediated photobiomodulation promotes healing and impedes inflammation, pain in full-thickness burn. J Photochem Photobiol B 186:152-159 (2018). PubMed: 30048845