Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475)
Key features and details
- Rabbit polyclonal to Sodium Potassium ATPase - Plasma Membrane Marker
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker
See all Sodium Potassium ATPase primary antibodies -
Description
Rabbit polyclonal to Sodium Potassium ATPase - Plasma Membrane Marker -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic non-phosphopeptide derived from human Sodium Potassium ATPase around the phosphorylation site of serine 16 (A-V-SP-E-H).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab58475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/50 - 1/100.
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| WB |
1/500 - 1/1000. Detects a band of approximately 113 kDa (predicted molecular weight: 113 kDa).
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| Notes |
|---|
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IHC-P
1/50 - 1/100. |
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WB
1/500 - 1/1000. Detects a band of approximately 113 kDa (predicted molecular weight: 113 kDa). |
Target
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Function
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. -
Sequence similarities
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. -
Post-translational
modificationsPhosphorylation on Tyr-10 modulates pumping activity. -
Cellular localization
Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 476 Human
- Entrez Gene: 480 Human
- Entrez Gene: 481 Human
- Omim: 182310 Human
- Omim: 182330 Human
- Omim: 607321 Human
- SwissProt: P05023 Human
- SwissProt: P05026 Human
see all -
Alternative names
- ATPase Na+/K+ transporting alpha antibody
- adenosinetriphosphatase antibody
- AT1A1_HUMAN antibody
see all
Images
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Western blot - Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475)All lanes : Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475) at 1/500 dilution
Lane 1 : 293 cell extracts treated with PMA (125ng/ml, 30mins)
Lane 2 : 293 cell extracts treated with PMA (125ng/ml, 30mins) with immunising peptide
Predicted band size: 113 kDa
Observed band size: 113 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475)ab58475 at 1/50 dilution staining Sodium Potassium ATPase in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (19)
ab58475 has been referenced in 19 publications.
- Li L & Zhang C Venlafaxine Attenuated the Cognitive and Memory Deficit in Mice Exposed to Isoflurane Alone. Front Neurol 12:591223 (2021). PubMed: 33708168
- Keshri GK et al. Effects of Pulsed 810?nm Al-Ga-As Diode Laser on Wound Healing Under Immunosuppression: A Molecular Insight. Lasers Surg Med 52:424-436 (2020). PubMed: 31483061
- Sharanek A et al. OSMR controls glioma stem cell respiration and confers resistance of glioblastoma to ionizing radiation. Nat Commun 11:4116 (2020). PubMed: 32807793
- Hao RT et al. NECTIN4 promotes papillary thyroid cancer cell proliferation, migration, and invasion and triggers EMT by activating AKT. Cancer Manag Res 11:2565-2578 (2019). PubMed: 31114323
- Fischer RA et al. Impairment of Membrane Repolarization Accompanies Axon Transport Deficits in Glaucoma. Front Neurosci 13:1139 (2019). PubMed: 31736686