Overview

  • Product name

    Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody
    See all Sodium/Hydrogen Exchanger 1/NHE-1 primary antibodies
  • Description

    Rabbit polyclonal to Sodium/Hydrogen Exchanger 1/NHE-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Sodium/Hydrogen Exchanger 1/NHE-1. (Derived from C-terminal cytoplasmic region).
    Database link: P19634

  • Positive control

    • IHC-P: Human kidney tissue. ICC/IF: HEK-293T cells transiently expressing Sodium/Hydrogen Exchanger 1/NHE-1. WB: Sodium/Hydrogen Exchanger 1/NHE-1 transfected HEK-293T cell lysate. Flow Cyt: Sodium/Hydrogen Exchanger 1/NHE-1 transfected HEK-293T cells.
  • General notes

    Previously labelled as Sodium / Hydrogen Exchanger 1.

Properties

Applications

Our Abpromise guarantee covers the use of ab230449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500. Predicted molecular weight: 91 kDa.
ICC/IF 1/100.
Flow Cyt 1/100.

Target

  • Function

    Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
  • Tissue specificity

    Kidney and intestine.
  • Sequence similarities

    Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
    O-glycosylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • amiloride-sensitive antibody
    • APNH antibody
    • APNH1 antibody
    • FLJ42224 antibody
    • Na Li countertransporter antibody
    • Na(+)/H(+) antiporter antibody
    • Na(+)/H(+) exchanger 1 antibody
    • Na+ H+ antiporter amiloride-sensitive antibody
    • Na+ H+ antiporter antibody
    • Na+ H+ exchanger 1 antibody
    • NHE-1 antibody
    • NHE1 antibody
    • OTTHUMP00000004468 antibody
    • SL9A1_HUMAN antibody
    • SLC9A1 antibody
    • Sodium / Hydrogen Exchanger 1 antibody
    • Sodium hydrogen exchanger 1 antibody
    • Sodium/hydrogen exchanger 1 antibody
    • solute carrier family 9 antibody
    • Solute carrier family 9 member 1 antibody
    • Solute carrier family 9 sodium hydrogen exchanger isoform 1 antiporter Na+ H+ amiloride sensitive antibody
    • Solute carrier family 9 subfamily A (NHE1 cation proton antiporter 1) member 1 antibody
    • Solute carrier family 9 subfamily A member 1 antibody
    see all

Images

  • All lanes : Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab230449) at 1/500 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : Myc-tagged Sodium/Hydrogen Exchanger 1/NHE-1 transfected HEK-293T cell lysate

    Predicted band size: 91 kDa

  • Formalin-fixed, paraffin-embedded human-kidney tissue stained for Sodium/Hydrogen Exchanger 1/NHE-1 using ab230449 at 1/100 dilution in immunohistochemical analysis.

  • HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transiently expressing Sodium/Hydrogen Exchanger 1/NHE-1 stained for Sodium/Hydrogen Exchanger 1/NHE-1 (red) using ab230449 at 1/100 dilution in ICC/IF. Left panel stained with Azami-green.

  • Flow cytometric analysis of intracellular Sodium/Hydrogen Exchanger 1/NHE-1 expression in HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transeintly expressing Sodium/Hydrogen Exchanger 1/NHE-1 and Azami green, using Normal rabbit IgG (left) and ab230449 at 1/100 dilution (right).

References

ab230449 has not yet been referenced specifically in any publications.

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