Key features and details
- Rabbit polyclonal to Sodium/Hydrogen Exchanger 1/NHE-1
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody
See all Sodium/Hydrogen Exchanger 1/NHE-1 primary antibodies
DescriptionRabbit polyclonal to Sodium/Hydrogen Exchanger 1/NHE-1
Tested applicationsSuitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Sodium/Hydrogen Exchanger 1/NHE-1. (Derived from C-terminal cytoplasmic region).
Database link: P19634
- IHC-P: Human kidney tissue. ICC/IF: HEK-293T cells transiently expressing Sodium/Hydrogen Exchanger 1/NHE-1. WB: Sodium/Hydrogen Exchanger 1/NHE-1 transfected HEK-293T cell lysate. Flow Cyt: Sodium/Hydrogen Exchanger 1/NHE-1 transfected HEK-293T cells.
Previously labelled as Sodium / Hydrogen Exchanger 1.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230449 in the following tested applications.
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/500. Predicted molecular weight: 91 kDa.|
FunctionInvolved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
Tissue specificityKidney and intestine.
Sequence similaritiesBelongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- amiloride-sensitive antibody
- APNH antibody
- APNH1 antibody
All lanes : Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab230449) at 1/500 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : Myc-tagged Sodium/Hydrogen Exchanger 1/NHE-1 transfected HEK-293T cell lysate
Predicted band size: 91 kDa
Formalin-fixed, paraffin-embedded human-kidney tissue stained for Sodium/Hydrogen Exchanger 1/NHE-1 using ab230449 at 1/100 dilution in immunohistochemical analysis.
HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transiently expressing Sodium/Hydrogen Exchanger 1/NHE-1 stained for Sodium/Hydrogen Exchanger 1/NHE-1 (red) using ab230449 at 1/100 dilution in ICC/IF. Left panel stained with Azami-green.
Flow cytometric analysis of intracellular Sodium/Hydrogen Exchanger 1/NHE-1 expression in HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transeintly expressing Sodium/Hydrogen Exchanger 1/NHE-1 and Azami green, using Normal rabbit IgG (left) and ab230449 at 1/100 dilution (right).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230449 has not yet been referenced specifically in any publications.