Key features and details
- Rabbit polyclonal to Sodium/Hydrogen Exchanger 1/NHE-1
- Suitable for: IHC-FoFr, WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody
See all Sodium/Hydrogen Exchanger 1/NHE-1 primary antibodies
DescriptionRabbit polyclonal to Sodium/Hydrogen Exchanger 1/NHE-1
Tested applicationsSuitable for: IHC-FoFr, WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human Sodium/Hydrogen Exchanger 1/NHE-1 (C terminal).
- Kidney. Brain.
Previously labelled as Sodium / Hydrogen Exchanger 1.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab67313 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 21705333|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa).|
|ICC/IF||Use a concentration of 20 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
FunctionInvolved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
Tissue specificityKidney and intestine.
Sequence similaritiesBelongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- amiloride-sensitive antibody
- APNH antibody
- APNH1 antibody
Lane 1 : Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67313) at 1 µg/ml
Lane 2 : Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67313) at 2 µg/ml
All lanes : Rat kidney tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 91 kDa
Observed band size: 91 kDa
Additional bands at: 50 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
ab67313 at 2.5 μg/ml staining of Sodium/Hydrogen Exchanger 1/NHE-1 in human brain tissue using IHC-P.
Immunofluorescence of Nhe-1 in Human Brain cells using ab67313 at 20 ug/ml.
ab67313 has been referenced in 2 publications.
- Kao L et al. Severe Neurologic Impairment in Mice with Targeted Disruption of the Electrogenic Sodium Bicarbonate Cotransporter NBCe2 (Slc4a5 Gene). J Biol Chem 286:32563-74 (2011). IHC-FoFr ; Mouse . PubMed: 21705333
- Uray KS et al. Sodium hydrogen exchanger as a mediator of hydrostatic edema-induced intestinal contractile dysfunction. Surgery 149:114-25 (2011). PubMed: 20553904