Product nameAnti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody
See all Sodium/Hydrogen Exchanger 1/NHE-1 primary antibodies
DescriptionRabbit polyclonal to Sodium/Hydrogen Exchanger 1/NHE-1
Tested applicationsSuitable for: ICC/IF, WB, IHC-FoFr, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human Sodium/Hydrogen Exchanger 1/NHE-1 (internal sequence).
- MOLT4 cell lysate. Human brain tissue.
Previously labelled as Sodium / Hydrogen Exchanger 1.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab67314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 20817726|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa).|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 20712402|
|IHC-P||Use at an assay dependent concentration.|
FunctionInvolved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
Tissue specificityKidney and intestine.
Sequence similaritiesBelongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- amiloride-sensitive antibody
- APNH antibody
- APNH1 antibody
Lane 1 : Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67314) at 1 µg/ml
Lane 2 : Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67314) at 2 µg/ml
All lanes : MOLT4 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 91 kDa
Observed band size: 91 kDa
Additional bands at: 48 kDa (possible isoform)
Immunofluorescence of Nhe-1 in Human Brain cells using ab67314 at 20 ug/ml.
This product has been referenced in:
- Yang D et al. Normobaric oxygen inhibits AQP4 and NHE1 expression in experimental focal ischemic stroke. Int J Mol Med 43:1193-1202 (2019). Read more (PubMed: 30592266) »
- Cheng PC et al. The Na+/H+-Exchanger NHE1 Regulates Extra- and Intracellular pH and Nimodipine-sensitive [Ca2+]i in the Suprachiasmatic Nucleus. Sci Rep 9:6430 (2019). Read more (PubMed: 31015514) »