Overview

  • Product name
    Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody
    See all Sodium/Hydrogen Exchanger 1/NHE-1 primary antibodies
  • Description
    Rabbit polyclonal to Sodium/Hydrogen Exchanger 1/NHE-1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-FoFr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Sodium/Hydrogen Exchanger 1/NHE-1 (internal sequence).

  • Positive control
    • MOLT4 cell lysate. Human brain tissue.
  • General notes

    Previously labelled as Sodium / Hydrogen Exchanger 1.

Properties

Applications

Our Abpromise guarantee covers the use of ab67314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20817726
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa).
IHC-FoFr Use at an assay dependent concentration. PubMed: 20712402
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
  • Tissue specificity
    Kidney and intestine.
  • Sequence similarities
    Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    O-glycosylated.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • amiloride-sensitive antibody
    • APNH antibody
    • APNH1 antibody
    • FLJ42224 antibody
    • Na Li countertransporter antibody
    • Na(+)/H(+) antiporter antibody
    • Na(+)/H(+) exchanger 1 antibody
    • Na+ H+ antiporter amiloride-sensitive antibody
    • Na+ H+ antiporter antibody
    • Na+ H+ exchanger 1 antibody
    • NHE-1 antibody
    • NHE1 antibody
    • OTTHUMP00000004468 antibody
    • SL9A1_HUMAN antibody
    • SLC9A1 antibody
    • Sodium / Hydrogen Exchanger 1 antibody
    • Sodium hydrogen exchanger 1 antibody
    • Sodium/hydrogen exchanger 1 antibody
    • solute carrier family 9 antibody
    • Solute carrier family 9 member 1 antibody
    • Solute carrier family 9 sodium hydrogen exchanger isoform 1 antiporter Na+ H+ amiloride sensitive antibody
    • Solute carrier family 9 subfamily A (NHE1 cation proton antiporter 1) member 1 antibody
    • Solute carrier family 9 subfamily A member 1 antibody
    see all

Images

  • Lane 1 : Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67314) at 1 µg/ml
    Lane 2 : Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67314) at 2 µg/ml

    All lanes : MOLT4 cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 91 kDa
    Observed band size: 91 kDa
    Additional bands at: 48 kDa (possible isoform)

  • Immunofluorescence of Nhe-1 in Human Brain cells using ab67314 at 20 ug/ml.

References

This product has been referenced in:
  • Yang D  et al. Normobaric oxygen inhibits AQP4 and NHE1 expression in experimental focal ischemic stroke. Int J Mol Med 43:1193-1202 (2019). Read more (PubMed: 30592266) »
  • Cheng PC  et al. The Na+/H+-Exchanger NHE1 Regulates Extra- and Intracellular pH and Nimodipine-sensitive [Ca2+]i in the Suprachiasmatic Nucleus. Sci Rep 9:6430 (2019). Read more (PubMed: 31015514) »
See all 10 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

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Application
Western blot
Sample
Dog Cell lysate - whole cell (Osteosarcoma derived from poodle)
Gel Running Conditions
Reduced Denaturing (7.5% gel)
Loading amount
30 µg
Treatment
150 µM amiloride, or 300 µM CoCl2, or vehicle
Specification
Osteosarcoma derived from poodle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

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Submitted Apr 23 2018

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