Overview

  • Product name

    Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody
    See all Sodium/Hydrogen Exchanger 3/NHE-3 primary antibodies
  • Description

    Rabbit polyclonal to Sodium/Hydrogen Exchanger 3/NHE-3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Rat Sodium/Hydrogen Exchanger 3/NHE-3 aa 809-831 (C terminal).
    Sequence:

    DSFLQADGPEEQLQPASPESTHM


    Database link: NP_036786

  • Positive control

    • Rat kidney tissue lysate.
  • General notes

    Previously labelled as Sodium / Hydrogen Exchanger 3. 

Properties

Applications

Our Abpromise guarantee covers the use of ab95299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 84 kDa (predicted molecular weight: 93 kDa).

Target

  • Function

    Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
  • Sequence similarities

    Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
  • Post-translational
    modifications

    Phosphorylated by PKA, which inhibits activity.
  • Cellular localization

    Membrane. In intestinal epithelial cells, localizes to the ileal brush border.
  • Information by UniProt
  • Database links

  • Alternative names

    • MGC126718 antibody
    • MGC126720 antibody
    • Na(+)/H(+) exchanger 3 antibody
    • NHE 3 antibody
    • NHE-3 antibody
    • NHE3 antibody
    • SL9A3_HUMAN antibody
    • SLC9A 3 antibody
    • Slc9a3 antibody
    • Sodium / Hydrogen Exchanger 3 antibody
    • Sodium/hydrogen exchanger 3 antibody
    • Sodium/hydrogen exchanger, apical epithelial antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger), member 3 antibody
    • Solute carrier family 9 member 3 antibody
    see all

Images

  • Western blot detection of Sodium/Hydrogen Exchanger 3 / NHE-3 in Rat kidney tissues using ab95299 at 1:1000 dilution.

References

This product has been referenced in:

  • Tingskov SJ  et al. Tamoxifen Decreases Lithium-Induced Natriuresis in Rats With Nephrogenic Diabetes Insipidus. Front Physiol 9:903 (2018). Read more (PubMed: 30050465) »
  • Wang YY  et al. SLC9A3 Protein Is Critical for Acrosomal Formation in Postmeiotic Male Germ Cells. Int J Mol Sci 19:N/A (2017). Read more (PubMed: 29286340) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Dog Cell lysate - whole cell (Osteosarcoma)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
30 µg
Specification
Osteosarcoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Aug 20 2018

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