Product nameAnti-SOFAT antibody
See all SOFAT primary antibodies
DescriptionGoat polyclonal to SOFAT
Specificityab211273 is expected to recognize isoform 1 (NP_060741.3) and isoform 2 (NP_001231605.1) only.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Hamster, Dog, Lizard, Opossum, Zebra finch, Platypus
- Human skeletal muscle tissue.
Previously labelled as THNSL2.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab211273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.
Preliminary experiments gave an approx 35kD band in Human Colorectal Cancer lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 45.0kD according to NP_001231605.1 and 54.1kD according to NP_060741.3. The 35kD band was successfully blocked by incubation with the immunizing peptide.
RelevanceSOFAT acts as a catabolic phospho-lyase on both gamma- and beta-phosphorylated substrates. It degrades O-phospho-threonine (PThr) to alpha-ketobutyrate, ammonia and phosphate (by similarity). There are 4 isoforms produced by alternative splicing.
- FLJ10916 antibody
- OTTHUMP00000160830 antibody
- OTTHUMP00000160831 antibody
ab211273 has not yet been referenced specifically in any publications.