Product nameAnti-SOFAT antibody
See all SOFAT primary antibodies
DescriptionRabbit polyclonal to SOFAT
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
- WB: HeLa and HepG2 whole cell lysates. IHC-P: Human ovarian cancer tissue.
Previously labelled as THNSL2.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.03% Proclin
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab235042 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 54 kDa.|
|IHC-P||1/200 - 1/500.|
RelevanceSOFAT acts as a catabolic phospho-lyase on both gamma- and beta-phosphorylated substrates. It degrades O-phospho-threonine (PThr) to alpha-ketobutyrate, ammonia and phosphate (by similarity). There are 4 isoforms produced by alternative splicing.
- FLJ10916 antibody
- OTTHUMP00000160830 antibody
- OTTHUMP00000160831 antibody
All lanes : Anti-SOFAT antibody (ab235042) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 54 kDa
Paraffin-embedded human ovarian cancer tissue stained for SOFAT using ab235042 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
ab235042 has not yet been referenced specifically in any publications.