Overview

  • Product name

    Anti-Solo antibody [EPR13772-74]
    See all Solo primary antibodies
  • Description

    Rabbit monoclonal [EPR13772-74] to Solo
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Solo aa 150-300. The exact sequence is proprietary.
    Database link: Q86VW0

  • Positive control

    • Human fetal brain tissue lysate; HepG2 and Raw264.7 cell lysates; mouse brain, mouse kidney and rat brain tissue lysates; Neuro-2a cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab192873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.
WB 1/1000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).

Target

  • Function

    May act as the primary docking protein directing membrane turnover and assembly of the transient receptor potential channels TRPC4 and TRPC5. Binds phospholipids such as phosphatidylinositol monophosphates, phosphatidylinositol diphosphates (PIP2s) and phosphatidic acid, but not less polar lipids including phosphatidylcholine, phosphatidylserine, and phosphatidylinositol. The binding to PIP2s is calcium dependent. Might be involved in the plasma membrane localization of CTNNB1.
  • Tissue specificity

    Broad expression. High expression in thalamus and brain. Significantly expressed in vasculature.
  • Sequence similarities

    Belongs to the SOLO family.
    Contains 1 CRAL-TRIO domain.
    Contains 3 spectrin repeats.
  • Information by UniProt
  • Database links

  • Alternative names

    • huntingtin interacting protein like protein antibody
    • Huntingtin-interacting protein-like protein antibody
    • Protein Solo antibody
    • SEC14 and spectrin domains 1 antibody
    • SEC14 domain and spectrin repeat-containing protein 1 antibody
    • SESD1_HUMAN antibody
    • sestd1 antibody
    • SOLO antibody
    see all

Images

  • Immunofluorescence analysis of Neuro2a cells labeling Solo using ab192873 at 1/50 dilution (20 μg/ml). A Goat anti rabbit IgG (Alexa Fluor®488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permealized with 0.1% triton X-100. Counterstain: DAPI.

  • All lanes : Anti-Solo antibody [EPR13772-74] (ab192873) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse kidney tissue lysate
    Lane 3 : Rat brain tissue lysate
    Lane 4 : Raw264.7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 79 kDa
    Observed band size: 79 kDa

  • All lanes : Anti-Solo antibody [EPR13772-74] (ab192873) at 1/1000 dilution

    Lane 1 : Human fetal brain tissue lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 79 kDa
    Observed band size: 79 kDa

References

ab192873 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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