Overview

  • Product name

    Soluble Epoxide Hydrolase Assay Kit
  • Detection method

    Fluorescent
  • Sample type

    Cell Lysate, Purified protein, Tissue Lysate
  • Product overview

    Soluble Epoxide Hydrolase Assay Kit (ab240999) is a microplate based fluorometric kit for measuring Soluble Epoxide Hydrolase (sEH) activity in cells and tissues as well as purified protein. It is based on the ability of sEH to hydrolyze a non-fluorescent substrate to a fluorescent product. The kit includes a specific inhibitor for soluble epoxide hydrolase, since the substrate can be hydrolyzed by non-specific hydrolases that are present in cell and tissue lysates. Specific sEH activity can be obtained by subtracting the activity in presence of sEH inhibitor form the total activity.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Fluorescence Standard 1 vial
    sEH Assay Buffer 1 x 25ml
    sEH Inhibitor 5X 1 x 100µl
    sEH Positive Control 1 vial
    sEH Substrate 1 x 200µl
  • Research areas

  • Function

    Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water.
  • Tissue specificity

    Found in liver.
  • Involvement in disease

    Note=In some populations, the high activity haplotype tyr113/his139 is overrepresented among women suffering from pregnancy-induced hypertension (pre-eclampsia) when compared with healthy controls.
    Defects in EPHX1 are a cause of familial hypercholanemia (FHCA) [MIM:607748]. FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption.
  • Sequence similarities

    Belongs to the peptidase S33 family.
  • Cellular localization

    Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Alternative names

    • EPHX
    • EPHX 1
    • Ephx1
    • EPOX
    • Epoxide hydratase
    • Epoxide hydrolase 1
    • Epoxide hydrolase 1 microsomal (xenobiotic)
    • Epoxide hydroxylase 1
    • Epoxide hydroxylase 1 microsomal (xenobiotic)
    • HYEP_HUMAN
    • HYL1
    • MEH
    • Microsomal epoxide hydrolase
    see all

Images

Protocols

References

ab240999 has not yet been referenced specifically in any publications.

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