- Datasheet
- Specific References
- Protocols
Overview
-
Product nameAnti-Soluble TNF Receptor II antibody
See all Soluble TNF Receptor II primary antibodies -
DescriptionRabbit polyclonal to Soluble TNF Receptor II
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, Indirect ELISA, Sandwich ELISA, Neutralisingmore details
-
Species reactivityReacts with: Human
-
Immunogen
Highly pure (>98%) recombinant Human Soluble TNF Receptor II.
Properties
-
FormLyophilised:Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/ml.
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
-
Storage bufferPreservative: None
Constituents: PBS, pH 7.2 -
Concentration information loading... -
PurityImmunogen affinity purified
-
Purification notesab109853 was purified by affinity chromatography employing immobilized Soluble TNF Receptor II matrix.
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
sELISA pair antibody
Applications
Our Abpromise guarantee covers the use of ab109853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 48 kDa. | |
| Indirect ELISA | Use a concentration of 0.5 - 2 µg/ml. (using 100 µl/well antibody solution). This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human soluble TNF Receptor II. | |
| Sandwich ELISA | Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Soluble TNF Receptor II (Biotin) (ab83158). (using 100 µl/well antibody solution). This antigen affinity purified antibody, in conjunction with ab83158 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human soluble TNF Receptor II. | |
| Neutralising | Use a concentration of 15 - 20 µg/ml. to yield one half maximal inhibition [ND50] of the biological activity of Human soluble TNF Receptor II (0.5 µg/ml) |
Target
-
FunctionReceptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
-
Sequence similaritiesContains 4 TNFR-Cys repeats.
-
Post-translational
modificationsPhosphorylated; mainly on serine residues and with a very low level on threonine residues.
A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing. -
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
-
Database links
- Entrez Gene: 7133 Human
- Omim: 191191 Human
- SwissProt: P20333 Human
- Unigene: 256278 Human
-
Alternative names
- CD120b antibody
- p75 antibody
- p75 TNF receptor antibody
see all
Protocols
References
ab109853 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"