Key features and details
- Rabbit polyclonal to Soluble TNF Receptor II
- Suitable for: WB, Sandwich ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Soluble TNF Receptor II antibody
See all Soluble TNF Receptor II primary antibodies
DescriptionRabbit polyclonal to Soluble TNF Receptor II
Tested applicationsSuitable for: WB, Sandwich ELISAmore details
Species reactivityReacts with: Human
Highly pure (>98%) recombinant Human Soluble TNF Receptor II.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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FormLyophilized:Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferConstituent: PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab109853 was purified by affinity chromatography employing immobilized Soluble TNF Receptor II matrix.
sELISA pair antibody
Our Abpromise guarantee covers the use of ab109853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 48 kDa.|
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Soluble TNF Receptor II (Biotin) (ab83158). (using 100 µl/well antibody solution). This antigen affinity purified antibody, in conjunction with ab83158 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human soluble TNF Receptor II.|
FunctionReceptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
Sequence similaritiesContains 4 TNFR-Cys repeats.
modificationsPhosphorylated; mainly on serine residues and with a very low level on threonine residues.
A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- CD120b antibody
- p75 antibody
- p75 TNF receptor antibody
ab109853 has not yet been referenced specifically in any publications.