Overview

  • Product name
    Anti-Somatostatin antibody [M09204]
    See all Somatostatin primary antibodies
  • Description
    Rat monoclonal [M09204] to Somatostatin
  • Host species
    Rat
  • Specificity
    This antibody recognizes somatostatin. It partially cross reacts with somatostatin fragments, but does not cross react with enkephalins, other endorphins, Substance P or CGRP. The clone number has been updated from (8.F.258) to (M09204) both clone numbers name the same antibody clone.
  • Tested applications
    Suitable for: ELISA, IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length protein corresponding to Human Somatostatin. Somatostatin conjugated to Thyroglobulin
    Database link: P61278

Properties

Applications

Our Abpromise guarantee covers the use of ab30788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr 1/25 - 1/100.
IHC-P 1/25 - 1/100. Antigen retrieval is recommended. Suitable for IHC(PFA fixed) PMID 21343251.
ICC/IF Use at an assay dependent concentration. PubMed: 23639079

Target

Images

  • ab30788 staining Somatostatin in mouse embryonic heart tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with Tween-20 and blocked with 5% serum for 60 minutes at 25°C. Samples were incubated with primary antibody (1/100) for 14 hours at 4°C. An Alexa Fluor®-conjugated Donkey anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Atanes P  et al. C3aR and C5aR1 act as key regulators of human and mouse ß-cell function. Cell Mol Life Sci 75:715-726 (2018). Read more (PubMed: 28921001) »
  • Li N  et al. Ablation of somatostatin cells leads to impaired pancreatic islet function and neonatal death in rodents. Cell Death Dis 9:682 (2018). Read more (PubMed: 29880854) »
See all 12 Publications for this product

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (islet)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mm sodium citrate PH 6
Permeabilization
No
Specification
islet
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 09 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrat-Buffer pH 6,0
Permeabilization
No
Specification
pancreas
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Nov 30 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mm sodium citrate pH6
Permeabilization
No
Specification
Pancreas
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 14 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Embryonic Heart)
Permeabilization
Yes - Tween-20
Specification
Embryonic Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 31 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
30 µg
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 20 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreas, langerhans islet, insulin + somatostatin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
Permeabilization
Yes - Tween-20
Specification
pancreas, langerhans islet, insulin + somatostatin
Blocking step
BSA as blocking agent for 40 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jun 23 2016

Answer




I am very pleased to hear you would like to accept our offer and test ab14993, ab30788 ,ab64053 and ab115489 in sELISA. These codes will give you: 1 freeprimary antibody per codebefore the expiration date. To redeem this offer, please submit an Abreview for sELISA and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you very much for your interest in PEDF and Somatostatin antibodies.

To our knowledge,none of our antibodies have been tested in sELISA. However, I have picked out four antibodies, which I found most suitable for this purpose:

PEDF

https://www.abcam.com/index.html?datasheet=14993 (or use the following: https://www.abcam.com/index.html?datasheet=14993).

https://www.abcam.com/index.html?datasheet=115489 (or use the following: https://www.abcam.com/index.html?datasheet=115489).

Somatostatin

https://www.abcam.com/index.html?datasheet=64053 (or use the following: https://www.abcam.com/index.html?datasheet=64053).

https://www.abcam.com/index.html?datasheet=30788 (or use the following: https://www.abcam.com/index.html?datasheet=30788).



I can offer a discount off a future purchase if you buyone or bothof these antibody couplesnow, test it insELISA and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody per antibody ordered.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab14993, ab30788, ab64053 andab115489 in sELISA. I will then send a discount code. This code must be issued before purchasing ab14993, ab30788, ab64053 and ab115489 so please wait for my reply before ordering.

2. Purchase ab14993, ab30788, ab64053 and ab115489 either by phone, fax, or online (www.abcam.com).

3. Test it in sELISA.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab14993, ab30788, ab64053 and ab115489 turns out to be unsuitable for sELISA, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for contacting us. The immunogen sequence of ab53165 is from xxxxxx of human somatostatin. We haven’t analyzed the Kd of this product. This antibody is guaranteed inICC/IF,WB,ELISA,IHC-P on mouse and human tissues. Ab35425 Sheep polyclonal antibody to somatostatin, was created by conjugating Somatostatin to keyhole limpet haemocyanin (KLH) using the glutaraldehyde method. While tested in RIA and ELISA, this has not been tested incapillary electrophoresis. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

Read More

Answer

Thank you for contacting us. The immunogen sequence of ab11770 includes amino acids XXX of Human Somatostatin. We haven’t analyzed the Kd of this product. According to our results, this antibody works well in ELISA and WB for purified recombinant fragment of Human Somatostatin. So we suggest you can test this antibody for competitive ELISA before your use in capillary electrophoresis. Our othermonoclonal antibody to human somatostatin, ab30788, recognizes a region within amino acids XXX of cyclic somatostatin. However this too has not been tested incapillary electrophoresis and also has not had the Kd analyzed. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

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1-10 of 11 Abreviews or Q&A

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