The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 16 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Receptor for somatostatins-14 and -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and PLC via pertussis toxin insensitive as well as sensitive G proteins. In RIN-5F cells, this receptor inhibits calcium entry by suppressing voltage dependent calcium-channels.
Cerebrum and kidney. In lesser amounts in jejunum, colon and liver.
Belongs to the G-protein coupled receptor 1 family.