Recombinant
RabMAb

Recombinant Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)

Rabbit recombinant monoclonal Somatostatin Receptor 2 antibody [UMB1]. Validated in WB, IP, IHC and tested in Mouse, Rat, Human. Cited in 43 publication(s).

Overview

  • Product name

    Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal
    See all Somatostatin Receptor 2 primary antibodies
  • Description

    Rabbit monoclonal [UMB1] to Somatostatin Receptor 2 - C-terminal
  • Host species

    Rabbit
  • Specificity

    The specificity of ab134152 was demonstrated in a Somatostatin Receptor 2-deficient mouse model. Refer to Fischer et al.
  • Tested applications

    Suitable for: IHC-Fr, WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Somatostatin Receptor 2 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P30874-1
    (Peptide available as ab171899)

  • Positive control

    • WB: Human and mouse brain tissue lysate. U937, INS-1 and Somatostatin Receptor 2 transfected HEK-293 cell lysates. IHC-P: Human pancreas and brain tissue.
  • General notes

      

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 23630299
WB 1/1000 - 1/10000. Detects a band of approximately 75-80 kDa (predicted molecular weight: 41 kDa).Can be blocked with Somatostatin Receptor 2 peptide (ab171899).

This antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Receptor for somatostatins-14 and -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and PLC via pertussis toxin insensitive as well as sensitive G proteins. In RIN-5F cells, this receptor inhibits calcium entry by suppressing voltage dependent calcium-channels.
    • Tissue specificity

      Cerebrum and kidney. In lesser amounts in jejunum, colon and liver.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Somatostatin receptor type 2 antibody
      • SRIF-1 antibody
      • SRIF1 antibody
      • SS-2-R antibody
      • SS2-R antibody
      • SS2R antibody
      • SSR2_HUMAN antibody
      • SST2 antibody
      • SSTR2 antibody
      see all

    Images

    • Agonist-induced internalization of sst2 (Somatostatin Receptor 2)  and sst5 (Somatostatin Receptor 5) tail-swap mutants

      Stably transfected HEK-293 cells were treated with 1 µM SS-14 for 0, 15 or 30 min. Cells were then fixed, stained with the anti-sst2 {UMB-1} (ab134152) or anti-sst5 antibody {UMB-4} (ab109495) and examined by confocal microscopy. Shown are representative images from one of at least three independent experiments. Scale bar, 20 µm.

      Cells were cells were fixed with 4% paraformaldehyde and 0.2% picric acid in phosphate buffer (pH 6.9) for 30 min at room temperature and washed several times. Cells were them permeabilized prior to incubation with the respective primary antibodies. Alexa® 488-conjugated secondary antibodies were used.

      (After Figure 2A of Lehmann et al)

    • Immunohistochemical analysis of paraffin embedded human brain tissue labeled with ab134152 at 1/100 dilution.

    • All lanes : Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152) at 1/1000 dilution

      Lane 1 : U937 (Human histiocytic lymphoma monocyte) whole cell lysates prepared in RIPA lysis method
      Lane 2 : U937 (Human histiocytic lymphoma monocyte) whole cell lysates prepared in 1% SDS hot lysis method
      Lane 3 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method
      Lane 4 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates prepared in 1% SDS hot lysis method
      Lane 5 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method
      Lane 6 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates prepared in 1% SDS hot lysis method
      Lane 7 : A549 (Human lung carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 41 kDa
      Observed band size: 73 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking and diluting buffer: 5% NFDM/TBST.

      This antibody doesn’t work in MCF-7 and MDA-MB-231 cells which should be positive (PMID: 16519802). A549 cell is Somatostatin Receptor 2 negative cell line (PMID: 19148511).

    • Immunohistochemical analysis of paraffin embedded human pancreas tissue labeled with ab134152 at 1/100 dilution.

    • All lanes : Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152) at 1/1000 dilution

      Lane 1 : Human brain lysates prepared in RIPA lysis method
      Lane 2 : Human brain lysates prepared in 1% SDS hot lysis method
      Lane 3 : Mouse brain lysates prepared in RIPA lysis method
      Lane 4 : Mouse brain lysates prepared in 1% SDS hot lysis method

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 41 kDa
      Observed band size: 73 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer: 5% NFDM/TBST.

      Exposure time:
      Lane 1 and 2: 1 second.
      Lane 3 and 4: 5 seconds.

    • All lanes : Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152) at 1/1000 dilution

      Lane 1 : U937 (Human histiocytic lymphoma cell line) cell lysates
      Lane 2 : INS-1 cell lysates
      Lane 3 : Somatostatin Receptor 2 transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 41 kDa

    References

    This product has been referenced in:

    • Carmona Matos DM  et al. Characterization of somatostatin receptors (SSTRs) expression and antiproliferative effect of somatostatin analogues in aggressive thyroid cancers. Surgery 165:64-68 (2019). Read more (PubMed: 30415874) »
    • Nakashima Y  et al. Clinicopathological characteristics of non-functioning cystic pancreatic neuroendocrine tumors. Pancreatology 19:50-56 (2019). Read more (PubMed: 30497875) »
    See all 52 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer

    The unprocessed Somatostatin Receptor 2 is about 41kDa, and then its molecular weight increases to ˜80 kDa at maturity.

    The specificity of the ab134152 was also examined in a sstr2-deficient mouse model. Please refer to the two publications attached (contact Scientific Support). They should help further clarify.

    Read More

    Answer



    This antibody, 3582-1/ab134152, reacts with an immunogen located at the C terminal residues of the canonical form of SSTR2. There is an alternate form of SSTR2 that expresses a different C-terminal sequence. Based on information in Uniprot, http://www.uniprot.org/uniprot/P30874#section_seq, it seems this antibody will not detect 'Isoform B' as defined on the webpage.

    Read More

    Answer

    I can confirm that there is no immunogenic homology with the SSTR2B protein so this antibody should bind to SSTR2A form only.

    Read More

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