Recombinant
RabMAb

Recombinant Anti-Somatostatin Receptor 2 antibody [UMB1] - Low endotoxin, Azide free (ab216649)

Overview

  • Product name

    Anti-Somatostatin Receptor 2 antibody [UMB1] - Low endotoxin, Azide free
    See all Somatostatin Receptor 2 primary antibodies
  • Description

    Rabbit monoclonal [UMB1] to Somatostatin Receptor 2 - Low endotoxin, Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Somatostatin Receptor 2 aa 350-450 (C terminal).
    (Peptide available as ab171899)

  • Positive control

    • WB: U937, INS-1, and sstr2 transfected HEK293 cell lysates. IHC-P: Human pancreas & brain tissue
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab216649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 23630299
WB Use at an assay dependent concentration. Detects a band of approximately 75-80 kDa (predicted molecular weight: 41 kDa).Can be blocked with Somatostatin Receptor 2 peptide (ab171899).
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Receptor for somatostatins-14 and -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and PLC via pertussis toxin insensitive as well as sensitive G proteins. In RIN-5F cells, this receptor inhibits calcium entry by suppressing voltage dependent calcium-channels.
    • Tissue specificity

      Cerebrum and kidney. In lesser amounts in jejunum, colon and liver.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Somatostatin receptor type 2 antibody
      • SRIF-1 antibody
      • SRIF1 antibody
      • SS-2-R antibody
      • SS2-R antibody
      • SS2R antibody
      • SSR2_HUMAN antibody
      • SST2 antibody
      • SSTR2 antibody
      see all

    Images

    • Agonist-induced internalization of sst2 (Somatostatin Receptor 2)  and sst5 (Somatostatin Receptor 5) tail-swap mutants

      Stably transfected HEK-293 cells were treated with 1 µM SS-14 for 0, 15 or 30 min. Cells were then fixed, stained with the anti-sst2 {UMB-1} (ab134152) or anti-sst5 antibody {UMB-4} (ab109495) and examined by confocal microscopy. Shown are representative images from one of at least three independent experiments. Scale bar, 20 µm.

      Cells were cells were fixed with 4% paraformaldehyde and 0.2% picric acid in phosphate buffer (pH 6.9) for 30 min at room temperature and washed several times. Cells were them permeabilized prior to incubation with the respective primary antibodies. Alexa® 488-conjugated secondary antibodies were used.

      (After Figure 2A of Lehmann et al)

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134152).

    • Immunohistochemical analysis of paraffin embedded human pancreas tissue labeled with ab134152 at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134152).

    • Immunohistochemical analysis of paraffin embedded human brain tissue labeled with ab134152 at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134152).

    References

    This product has been referenced in:

    • Menke JR  et al. Somatostatin receptor 2a is a more sensitive diagnostic marker of meningioma than epithelial membrane antigen. Acta Neuropathol N/A:N/A (2015). IHC-P, IHC ; Human . Read more (PubMed: 26195322) »
    • Sadowski SM  et al. Detection of insulinoma using (68)Gallium-DOTATATE PET/CT: a case report. Gland Surg 3:E1-5 (2014). IHC ; Human . Read more (PubMed: 25493261) »
    See all 13 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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