Anti-Somatostatin Receptor 3/SSTR3 antibody [7H8E5] (ab201952)
Key features and details
- Mouse monoclonal [7H8E5] to Somatostatin Receptor 3/SSTR3
- Suitable for: WB, Flow Cyt
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Somatostatin Receptor 3/SSTR3 antibody [7H8E5]
See all Somatostatin Receptor 3/SSTR3 primary antibodies -
Description
Mouse monoclonal [7H8E5] to Somatostatin Receptor 3/SSTR3 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment corresponding to Human Somatostatin Receptor 3/SSTR3 aa 1-100. Recombinant fragment corresponding to extracellular domain of human Somatostatin Receptor 3/SSTR3 expressed in E coli
Database link: P32745 -
Positive control
- Human Somatostatin Receptor 3/SSTR3 (AA: 1-43) recombinant protein; Human Somatostatin Receptor 3/SSTR3 (AA: 1-43) transfected HEK293 cell lysate; HeLA; PANC1; PC-12; SK N SH; U937 and HepG2 whole cell lysate (ab7900). HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
7H8E5 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab201952 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 46 kDa.
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Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 46 kDa. |
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Receptor for somatostatins-14 and -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. -
Tissue specificity
Brain, pituitary and pancreas. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsPhosphorylated. Phosphorylation increases upon somatostatin binding. -
Cellular localization
Cell membrane. Internalized into endoplasmic vesicles upon somatostatin-stimulation. - Information by UniProt
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Database links
- Entrez Gene: 6753 Human
- Entrez Gene: 171044 Rat
- Omim: 182453 Human
- SwissProt: P32745 Human
- SwissProt: P30936 Rat
- Unigene: 225995 Human
- Unigene: 10458 Rat
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Alternative names
- OTTHUMP00000028737 antibody
- Smstr 3 antibody
- Smstr3 antibody
see all
Images
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Flow cytometric analysis of Hela cells labeling Smoatostatin Receptor 3 using ab 201952 at 1/200 dilution (green) and negative control (red).
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All lanes : Anti-Somatostatin Receptor 3/SSTR3 antibody [7H8E5] (ab201952) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : PANC1 cell lysate
Lane 3 : PC 12 cell lysate
Lane 4 : SK N SH cell lysate
Lane 5 : U937 cell lysate
Lane 6 : HepG2 cell lysate
Predicted band size: 46 kDa -
All lanes : Anti-Somatostatin Receptor 3/SSTR3 antibody [7H8E5] (ab201952) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Human Somatostatin Receptor 3/SSTR3 (AA: 1-43) recombinant protein trasfected HEK293 cell lysate
Predicted band size: 46 kDa -
Anti-Somatostatin Receptor 3/SSTR3 antibody [7H8E5] (ab201952) at 1/500 dilution + Human Somatostatin Receptor 3/SSTR3 (AA: 1-43) recombinant protein
Predicted band size: 46 kDaExpected MW is 30.2 kD
Datasheets and documents
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Datasheet download
References (0)
ab201952 has not yet been referenced specifically in any publications.