Recombinant
RabMAb

Recombinant Anti-Somatostatin Receptor 3/SSTR3 antibody [UMB5] (ab137026)

Overview

  • Product name

    Anti-Somatostatin Receptor 3/SSTR3 antibody [UMB5]
    See all Somatostatin Receptor 3/SSTR3 primary antibodies
  • Description

    Rabbit monoclonal [UMB5] to Somatostatin Receptor 3/SSTR3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Somatostatin Receptor 3/SSTR3 aa 350-450 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Somatostatin Receptor 3/SSTR3 transfected 293T cell lysate
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Somatostatin Receptor 3

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 46 kDa.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Receptor for somatostatins-14 and -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase.
    • Tissue specificity

      Brain, pituitary and pancreas.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.
    • Post-translational
      modifications

      Phosphorylated. Phosphorylation increases upon somatostatin binding.
    • Cellular localization

      Cell membrane. Internalized into endoplasmic vesicles upon somatostatin-stimulation.
    • Information by UniProt
    • Database links

    • Alternative names

      • OTTHUMP00000028737 antibody
      • Smstr 3 antibody
      • Smstr3 antibody
      • Somatostatin receptor subtype 3 antibody
      • Somatostatin receptor type 3 antibody
      • SS 3R antibody
      • SS-3-R antibody
      • SS3-R antibody
      • SS3R antibody
      • SSR 28 antibody
      • SSR-28 antibody
      • SSR28 antibody
      • SSR3_HUMAN antibody
      • Sst 3 antibody
      • Sst3 antibody
      • SSTR 3 antibody
      • SSTR3 antibody
      see all

    Images

    • Overlay histogram showing SHSY-5Y cells stained with ab137026 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137026, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • All lanes : Anti-Somatostatin Receptor 3/SSTR3 antibody [UMB5] (ab137026) at 1/10000 dilution

      Lane 1 : Non-transfected 293T cell lysate
      Lane 2 : Somatostatin Receptor 3/SSTR3 transfected 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 46 kDa

    References

    This product has been referenced in:

    • Zou Y  et al. Expression and selective activation of somatostatin receptor subtypes induces cell cycle arrest in cancer cells. Oncol Lett 17:1723-1731 (2019). Read more (PubMed: 30675231) »
    • Venegas-Moreno E  et al. Association between dopamine and somatostatin receptor expression and pharmacological response to somatostatin analogues in acromegaly. J Cell Mol Med 22:1640-1649 (2018). IHC ; Human . Read more (PubMed: 29266696) »
    See all 8 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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