Overview

  • Product name
    Anti-Somatostatin Receptor 5 antibody
    See all Somatostatin Receptor 5 primary antibodies
  • Description
    Rabbit polyclonal to Somatostatin Receptor 5
  • Host species
    Rabbit
  • Specificity
    ab28618 recognises Somatostatin Receptor 5.
  • Tested applications
    Suitable for: IHC-P, ICC, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Somatostatin Receptor 5 aa 344-364.
    Sequence:

    QEATRPAHRAAANGLMQTSKL

Properties

Applications

Our Abpromise guarantee covers the use of ab28618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2500.
ICC 1/5000.
WB 1/5000. Detects a band of approximately 54-63 kDa.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Receptor for somatostatin 28 and to a lesser extent for somatostatin-14. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
  • Tissue specificity
    Adult pituitary gland, heart, small intestine, adrenal gland, cerebellum and fetal hypothalamus. No expression in fetal or adult kidney, liver, pancreas, uterus, spleen, lung, thyroid or ovary.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • SSTR5 antibody
    • Somatostatin receptor subtype 5 antibody
    • Somatostatin receptor type 5 antibody
    • SS 5R antibody
    • SS-5-R antibody
    • SS5 R antibody
    • SS5-R antibody
    • SS5R antibody
    • SSR5_HUMAN antibody
    • SST R5 antibody
    • SSTR 5 antibody
    • Sstr5 antibody
    see all

Images

  • Anti-Somatostatin Receptor 5 antibody (ab28618) at 1 µg/ml + Brain: pituitary gland (Human) Whole Cell Lysate - adult normal tissue (ab29749) at 10 µg

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 42 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 15 kDa, 20 kDa, 73 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes


    Somatostatin Receptor 5 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • ICC/IF image of ab28618 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28618, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Gonzalez B  et al. Cytoplasmic expression of SSTR2 and 5 by immunohistochemistry and by RT/PCR is not associated with the pharmacological response to octreotide. Endocrinol Nutr 61:523-30 (2014). Read more (PubMed: 25008035) »
  • Liu Y  et al. Somatostatin receptor subtypes 2 and 5 are associated with better survival in operable hepatitis B-related hepatocellular carcinoma following octreotide long-acting release treatment. Oncol Lett 6:821-828 (2013). Read more (PubMed: 24137418) »
See all 7 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you vary much for your patience while I contacted the products originator. According to the original researcher (and author of the paper that I provided), "most GPCRs are N-glycosylated proteins which accounts for 10 to 30 kDa higher molecular weight than expected. This N-glycosylation should be used to enrich the receptors using wheat germ lectin agarose beads before western blot just as described in the CCR paper. The receptor can be deglycosylated using PNGase F. Then the molecular weight should shrink to the expected size. (see attached paper Fig 1B)." I hope that this information will help your customer to understand why the calculated versus apparent molecular weight may be different. Please let me know if you have any additional questions.

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Answer

Thank you for your enquiry. In Charlotte's absence I am handling her enquiries. According to the original researcher (and author of the paper that Charlotte provided you with), "most GPCRs are N-glycosylated proteins which accounts for 10 to 30 kDa higher molecular weight than expected. This N-glycosylation should be used to enrich the receptors using wheat germ lectin agarose beads before western blot just as described in the CCR paper. The receptor can be deglycosylated using PNGase F. Then the molecular weight should shrink to the expected size. (see attached paper Fig 1B)." I hope that this goes some way to explaining why the calculated versus apparent molecular weight may be different. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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