Recombinant
RabMAb

Recombinant Anti-Somatostatin Receptor 5 antibody [EPR7243] (ab156864)

Overview

  • Product name

    Anti-Somatostatin Receptor 5 antibody [EPR7243]
    See all Somatostatin Receptor 5 primary antibodies
  • Description

    Rabbit monoclonal [EPR7243] to Somatostatin Receptor 5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human Somatostatin Receptor 5 (UniProt: P35346).

  • Positive control

    • HeLa cells; Human fetal kidney, Human kidney, Human fetal liver, Human spleen, HepG2, HT29, 293T, HeLa, Human fetal heart and Human small intestine cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab156864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Receptor for somatostatin 28 and to a lesser extent for somatostatin-14. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
    • Tissue specificity

      Adult pituitary gland, heart, small intestine, adrenal gland, cerebellum and fetal hypothalamus. No expression in fetal or adult kidney, liver, pancreas, uterus, spleen, lung, thyroid or ovary.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • SSTR5 antibody
      • Somatostatin receptor subtype 5 antibody
      • Somatostatin receptor type 5 antibody
      • SS 5R antibody
      • SS-5-R antibody
      • SS5 R antibody
      • SS5-R antibody
      • SS5R antibody
      • SSR5_HUMAN antibody
      • SST R5 antibody
      • SSTR 5 antibody
      • Sstr5 antibody
      see all

    Images

    • All lanes : Anti-Somatostatin Receptor 5 antibody [EPR7243] (ab156864) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human kidney lysate
      Lane 3 : Human fetal liver lysate
      Lane 4 : Human spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 39 kDa

    • All lanes : Anti-Somatostatin Receptor 5 antibody [EPR7243] (ab156864) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HT29 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HeLa cell lysate
      Lane 5 : Human fetal heart cell lysate
      Lane 6 : Human small intestine lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 39 kDa

    • Flow cytometric analysis of HeLa cells labeling Somatostatin receptor type 5 using ab156864 at 1/100 (red) or a rabbit IgG (negative) (green).

    References

    This product has been referenced in:

    • Lee J  et al. Effect of the Orally Active Growth Hormone Secretagogue MK-677 on Somatic Growth in Rats. Yonsei Med J 59:1174-1180 (2018). Read more (PubMed: 30450851) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% gel)
    Loading amount
    25 µg
    Treatment
    NT or SSTR5 siRNA
    Specification
    HeLa cells
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted May 16 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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