Recombinant
RabMAb

Recombinant Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495)

Overview

  • Product name

    Anti-Somatostatin Receptor 5 antibody [UMB4]
    See all Somatostatin Receptor 5 primary antibodies
  • Description

    Rabbit monoclonal [UMB4] to Somatostatin Receptor 5
  • Host species

    Rabbit
  • Specificity

    Internal WB test in PANC-1, human pancreas and human brain lysates indicates this antibody might not detect endogenous Somatostatin Receptor 5.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Somatostatin Receptor 5 aa 300-400 (C terminal). The exact sequence is proprietary.
    (Peptide available as ab178801)

  • Positive control

    • WB: HT29 whole cell lysate (ab3952), 293T (Human embryonic kidney epithelial cell) transfected with human SSTR5 expression vector, IHC-P: Human pancreas
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.Can be blocked with Somatostatin Receptor 5 peptide (ab178801).

Internal WB test in PANC-1, human pancreas and human brain lysates indicates this antibody might not detect endogenous Somatostatin Receptor 5.

IHC-P 1/50. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

For unpurified use at 1/100-1/250

See IHC antigen retrieval protocols.

  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Receptor for somatostatin 28 and to a lesser extent for somatostatin-14. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
    • Tissue specificity

      Adult pituitary gland, heart, small intestine, adrenal gland, cerebellum and fetal hypothalamus. No expression in fetal or adult kidney, liver, pancreas, uterus, spleen, lung, thyroid or ovary.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • SSTR5 antibody
      • Somatostatin receptor subtype 5 antibody
      • Somatostatin receptor type 5 antibody
      • SS 5R antibody
      • SS-5-R antibody
      • SS5 R antibody
      • SS5-R antibody
      • SS5R antibody
      • SSR5_HUMAN antibody
      • SST R5 antibody
      • SSTR 5 antibody
      • Sstr5 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human pancreas tissue sections labeling Somatostatin Receptor 5 with Purified ab109495 at 1:50 dilution (16.3 µg/ml). Heat mediated antigen retrieval was performed with Tris/EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins.. Performed on a Leica Biosystems BOND ® RX instrument.was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
    • Somatostatin receptor 2 (SSTR2), somatostatin receptor 5 (SSTR5) and CXCR4 expression in the hepatoblastoma cell line HepG2 and in the hepatoma cell lines Hep3B and HuH-7 in comparison to the small cell lung cancer cell line NCI-H69 and the neuroendocrine tumor cell line BON-1. Immunohistochemistry (red-brown color), counterstaining with hematoxylin; scale bar: 20 μm. Representative photomicrographs of three independent batches are shown.

      HepG2, Hep3B, HuH-7, NCI-H-69, and BON-1 cells (DSMZ, Braunschweig, Germany) were grown in 75 cm2 culture flasks to a confluency of 80%. Cells were washed once with phosphate-buffered saline and transferred into 10% buffered formalin (J.T.Baker, Deventer, The Netherlands) for 2 h. After centrifugation for 10 min at 3500 x g, the supernatant was removed, and 1 ml human pool plasma was added to the cell samples. After brief vortexing, 100 μl human fibrinogen (50–70% protein; ≥80% clottable) was added to each sample, and the samples were vortexed again. The resulting clots were placed for another 24 h in 10% buffered formalin and embedded in paraffin blocks.

      (After Kaemmerer, D. et al BMC Cancer. 2017 Dec 28;17(1):896. doi: 10.1186/s12885-017-3911-3).

      Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/)

       

    • Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495) at 0.08 µg/ml (purified) + 293T (Human embryonic kidney epithelial cell) transfected with human SSTR5 expression vector, whole cell lysate at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 39 kDa



      Blocking and diluting buffer: 5% NFDM/TBST

    References

    This product has been referenced in:

    • Zou Y  et al. Expression and selective activation of somatostatin receptor subtypes induces cell cycle arrest in cancer cells. Oncol Lett 17:1723-1731 (2019). Read more (PubMed: 30675231) »
    • Venegas-Moreno E  et al. Association between dopamine and somatostatin receptor expression and pharmacological response to somatostatin analogues in acromegaly. J Cell Mol Med 22:1640-1649 (2018). IHC ; Human . Read more (PubMed: 29266696) »
    See all 22 Publications for this product

    Customer reviews and Q&As

    Question
    Answer


    J’ai contacté le laboratoire qui a accepté d’ajouter le peptide bloquant d’ab109495 au catalogue sous la référence ab178801.

    Read More

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