Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [UMB4] to Somatostatin Receptor 5
- Suitable for: WB, IHC-P
- Reacts with: Human
Product nameAnti-Somatostatin Receptor 5 antibody [UMB4]
See all Somatostatin Receptor 5 primary antibodies
DescriptionRabbit monoclonal [UMB4] to Somatostatin Receptor 5
SpecificityInternal WB test in PANC-1, human pancreas and human brain lysates indicates this antibody might not detect endogenous Somatostatin Receptor 5.
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF
Species reactivityReacts with: Human
Synthetic peptide within Human Somatostatin Receptor 5 aa 300-400 (C terminal). The exact sequence is proprietary.
(Peptide available as
- WB: HT29 whole cell lysate (ab3952), 293T (Human embryonic kidney epithelial cell) transfected with human SSTR5 expression vector, IHC-P: Human pancreas
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
PurityProtein A purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab109495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 39 kDa.Can be blocked with Somatostatin Receptor 5 peptide (ab178801).
Internal WB test in PANC-1, human pancreas and human brain lysates indicates this antibody might not detect endogenous Somatostatin Receptor 5.
|IHC-P||1/50. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
For unpurified use at 1/100-1/250
FunctionReceptor for somatostatin 28 and to a lesser extent for somatostatin-14. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
Tissue specificityAdult pituitary gland, heart, small intestine, adrenal gland, cerebellum and fetal hypothalamus. No expression in fetal or adult kidney, liver, pancreas, uterus, spleen, lung, thyroid or ovary.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- SSTR5 antibody
- Somatostatin receptor subtype 5 antibody
- Somatostatin receptor type 5 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human pancreas tissue sections labeling Somatostatin Receptor 5 with Purified ab109495 at 1:50 dilution (16.3 µg/ml). Heat mediated antigen retrieval was performed with Tris/EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins.. Performed on a Leica Biosystems BOND ® RX instrument.was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
Somatostatin receptor 2 (SSTR2), somatostatin receptor 5 (SSTR5) and CXCR4 expression in the hepatoblastoma cell line HepG2 and in the hepatoma cell lines Hep3B and HuH-7 in comparison to the small cell lung cancer cell line NCI-H69 and the neuroendocrine tumor cell line BON-1. Immunohistochemistry (red-brown color), counterstaining with hematoxylin; scale bar: 20 μm. Representative photomicrographs of three independent batches are shown.
HepG2, Hep3B, HuH-7, NCI-H-69, and BON-1 cells (DSMZ, Braunschweig, Germany) were grown in 75 cm2 culture flasks to a confluency of 80%. Cells were washed once with phosphate-buffered saline and transferred into 10% buffered formalin (J.T.Baker, Deventer, The Netherlands) for 2 h. After centrifugation for 10 min at 3500 x g, the supernatant was removed, and 1 ml human pool plasma was added to the cell samples. After brief vortexing, 100 μl human fibrinogen (50–70% protein; ≥80% clottable) was added to each sample, and the samples were vortexed again. The resulting clots were placed for another 24 h in 10% buffered formalin and embedded in paraffin blocks.
(After Kaemmerer, D. et al BMC Cancer. 2017 Dec 28;17(1):896. doi: 10.1186/s12885-017-3911-3).
Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/)
Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495) at 0.08 µg/ml (purified) + 293T (Human embryonic kidney epithelial cell) transfected with human SSTR5 expression vector, whole cell lysate at 15 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 39 kDa
Blocking and diluting buffer: 5% NFDM/TBST
ab109495 has been referenced in 24 publications.
- Ilie MD et al. Gonadotroph Tumors Show Subtype Differences That Might Have Implications for Therapy. Cancers (Basel) 12:N/A (2020). PubMed: 32326042
- Lundsten S et al. The HSP90 inhibitor onalespib potentiates 177Lu-DOTATATE therapy in neuroendocrine tumor cells. Int J Oncol 55:1287-1295 (2019). PubMed: 31638190
- Zou Y et al. Expression and selective activation of somatostatin receptor subtypes induces cell cycle arrest in cancer cells. Oncol Lett 17:1723-1731 (2019). PubMed: 30675231
- Venegas-Moreno E et al. Association between dopamine and somatostatin receptor expression and pharmacological response to somatostatin analogues in acromegaly. J Cell Mol Med 22:1640-1649 (2018). IHC ; Human . PubMed: 29266696
- Harda K et al. Somatostatin Receptors as Molecular Targets in Human Uveal Melanoma. Molecules 23:N/A (2018). PubMed: 29949880