Overview

  • Product name
  • Description
    Rabbit polyclonal to SON
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 1463-1598 of Human SON.

  • Positive control
    • RT4, U-251 MG cell lysates; Human small intestine tissue; U-251 MG cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab121759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

Target

  • Function
    Represses hepatitis B virus (HBV) core promoter activity and transcription of HBV genes and production of HBV virions. Binds to the consensus DNA sequence: 5'-GA[GT]AN[CG][AG]CC-3'. Might protect cells from apoptosis. Might be involved in pre-mRNA splicing.
  • Tissue specificity
    Widely expressed, with the higher expression seen in leukocyte and heart.
  • Sequence similarities
    Contains 1 DRBM (double-stranded RNA-binding) domain.
    Contains 1 G-patch domain.
  • Domain
    Contains 8 types of repeats which are distributed in 3 regions.
  • Cellular localization
    Nucleus speckle. Colocalizes with the pre-mRNA splicing factor SFRS2/SC-35.
  • Information by UniProt
  • Database links
  • Alternative names
    • BASS1 antibody
    • Bax antagonist selected in saccharomyces 1 antibody
    • DBP-5 antibody
    • Negative regulatory element-binding protein antibody
    • NRE-binding protein antibody
    • Protein DBP-5 antibody
    • Protein SON antibody
    • SON antibody
    • SON protein antibody
    • SON_HUMAN antibody
    • SON3 antibody
    see all

Images

  • ab121759 at 1/1200 dilution staining SON in Paraffin-embedded Human small intestine tissue by Immunohistochemistry.
  • ab121759, at 4 µg/ml, staining SON (green) in PFA-fixed, Triton X-100 permbabilized U-251 MG cells, by Immunofluorescence.

References

ab121759 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293T)
Permeabilization
Yes - 0.1% Triton X100 in PBA
Specification
HEK293T
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
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Submitted Mar 15 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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