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    sondbp5-antibody-ab121759.pdf

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Cell Biology Cell Cycle Cell Division Spindle
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Anti-SON/DBP5 antibody (ab121759)

  • Datasheet
Reviews (1) Submit a question References (3)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (ab121759)
  • Immunocytochemistry - Anti-SON/DBP5 antibody (ab121759)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (ab121759)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (ab121759)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (ab121759)

Key features and details

  • Rabbit polyclonal to SON/DBP5
  • Suitable for: ICC, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-SON/DBP5 antibody
  • Description

    Rabbit polyclonal to SON/DBP5
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC
    Human
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human SON/DBP5 aa 1463-1598.

  • Positive control

    • IHC-P: Human small intestine, tonsil, prostate, and cerebellum tissue. ICC: U-251 MG cell lysates
  • General notes

     This product was previously labelled as SON

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Spindle
    • Cell Biology
    • Apoptosis
    • Nucleus
    • Other
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Chromosome Structure
    • Centromere
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Apoptosis
    • Nuclear
    • Cancer
    • Cell cycle
    • Cell division
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • Other
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab121759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC
Human
IHC-P
Human
Application Abreviews Notes
ICC
Use a concentration of 0.25 - 2 µg/ml.
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
ICC
Use a concentration of 0.25 - 2 µg/ml.
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Represses hepatitis B virus (HBV) core promoter activity and transcription of HBV genes and production of HBV virions. Binds to the consensus DNA sequence: 5'-GA[GT]AN[CG][AG]CC-3'. Might protect cells from apoptosis. Might be involved in pre-mRNA splicing.
  • Tissue specificity

    Widely expressed, with the higher expression seen in leukocyte and heart.
  • Sequence similarities

    Contains 1 DRBM (double-stranded RNA-binding) domain.
    Contains 1 G-patch domain.
  • Domain

    Contains 8 types of repeats which are distributed in 3 regions.
  • Cellular localization

    Nucleus speckle. Colocalizes with the pre-mRNA splicing factor SFRS2/SC-35.
  • Target information above from: UniProt accession P18583 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6651 Human
    • Omim: 182465 Human
    • SwissProt: P18583 Human
    • Unigene: 517262 Human
    • Alternative names

      • BASS1 antibody
      • Bax antagonist selected in saccharomyces 1 antibody
      • DBP-5 antibody
      • Negative regulatory element-binding protein antibody
      • NRE-binding protein antibody
      • Protein DBP-5 antibody
      • Protein SON antibody
      • SON antibody
      • SON protein antibody
      • SON_HUMAN antibody
      • SON3 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (ab121759)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (ab121759)

      Paraffin-embedded human small intestine tissue staining SON/DBP5 with ab121759 at 1/200 dilution in immunohistochemical analysis. Moderate to strong postiivty in nuclear speckles in glandular cells.

    • Immunocytochemistry - Anti-SON/DBP5 antibody (ab121759)
      Immunocytochemistry - Anti-SON/DBP5 antibody (ab121759)

      ab121759, at 4 µg/ml, staining SON/DBP5 (green) in PFA-fixed, Triton X-100 permbabilized U-251 MG cells, by Immunofluorescence.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (ab121759)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (ab121759)

      Paraffin-embedded human prostate tissue staining SON/DBP5 with ab121759 at 1/200 dilution in immunohistochemical analysis. Moderate to strong positivity in nuclear speckles in glandular cells.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (ab121759)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (ab121759)

      Paraffin-embedded human tonsil tissue staining SON/DBP5 with ab121759 at 1/200 dilution in immunohistochemical analysis. Moderate to strong positivity in nuclear speckles in germinal center cells.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (ab121759)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (ab121759)

      Paraffin-embedded human cerebellum tissue staining SON/DBP5 with ab121759 at 1/200 dilution in immunohistochemical analysis. Moderate to strong positivity in nuclear speckles in Purkinje cells.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (3)

    Publishing research using ab121759? Please let us know so that we can cite the reference in this datasheet.

    ab121759 has been referenced in 3 publications.

    • Fáberová V  et al. Super-Resolution Localisation of Nuclear PI(4)P and Identification of Its Interacting Proteome. Cells 9:N/A (2020). PubMed: 32403279
    • Kim JH  et al. SON haploinsufficiency causes impaired pre-mRNA splicing of CAKUT genes and heterogeneous renal phenotypes. Kidney Int 95:1494-1504 (2019). PubMed: 31005274
    • Park R & Miller G Epstein-Barr Virus-Induced Nodules on Viral Replication Compartments Contain RNA Processing Proteins and a Viral Long Noncoding RNA. J Virol 92:N/A (2018). PubMed: 30068640

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry/ Immunofluorescence abreview for Anti-SON antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK293T)
    Permeabilization
    Yes - 0.1% Triton X100 in PBA
    Specification
    HEK293T
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 15 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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