Anti-SON/DBP5 antibody (ab121759)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-SON/DBP5 antibody
See all SON/DBP5 primary antibodies -
Description
Rabbit polyclonal to SON/DBP5 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human SON/DBP5 aa 1463-1598.
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Positive control
- IHC-P: Human small intestine, tonsil, prostate, and cerebellum tissue ICC/IF: U-251 MG cell lysates
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General notes
This product was previously labelled as SON
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab121759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
Target
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Function
Represses hepatitis B virus (HBV) core promoter activity and transcription of HBV genes and production of HBV virions. Binds to the consensus DNA sequence: 5'-GA[GT]AN[CG][AG]CC-3'. Might protect cells from apoptosis. Might be involved in pre-mRNA splicing. -
Tissue specificity
Widely expressed, with the higher expression seen in leukocyte and heart. -
Sequence similarities
Contains 1 DRBM (double-stranded RNA-binding) domain.
Contains 1 G-patch domain. -
Domain
Contains 8 types of repeats which are distributed in 3 regions. -
Cellular localization
Nucleus speckle. Colocalizes with the pre-mRNA splicing factor SFRS2/SC-35. - Information by UniProt
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Database links
- Entrez Gene: 6651 Human
- Omim: 182465 Human
- SwissProt: P18583 Human
- Unigene: 517262 Human
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Alternative names
- BASS1 antibody
- Bax antagonist selected in saccharomyces 1 antibody
- DBP-5 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (ab121759)Paraffin-embedded human small intestine tissue staining SON/DBP5 with ab121759 at 1/200 dilution in immunohistochemical analysis. Moderate to strong postiivty in nuclear speckles in glandular cells.
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Immunocytochemistry/ Immunofluorescence - Anti-SON/DBP5 antibody (ab121759)ab121759, at 4 µg/ml, staining SON/DBP5 (green) in PFA-fixed, Triton X-100 permbabilized U-251 MG cells, by Immunofluorescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (ab121759)Paraffin-embedded human prostate tissue staining SON/DBP5 with ab121759 at 1/200 dilution in immunohistochemical analysis. Moderate to strong positivity in nuclear speckles in glandular cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (ab121759)Paraffin-embedded human tonsil tissue staining SON/DBP5 with ab121759 at 1/200 dilution in immunohistochemical analysis. Moderate to strong positivity in nuclear speckles in germinal center cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (ab121759)Paraffin-embedded human cerebellum tissue staining SON/DBP5 with ab121759 at 1/200 dilution in immunohistochemical analysis. Moderate to strong positivity in nuclear speckles in Purkinje cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (ab121759)ab121759 at 1/1200 dilution staining SON/DBP5 in Paraffin-embedded Human small intestine tissue by Immunohistochemistry.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Kim JH et al. SON haploinsufficiency causes impaired pre-mRNA splicing of CAKUT genes and heterogeneous renal phenotypes. Kidney Int 95:1494-1504 (2019). Read more (PubMed: 31005274) »
- Park R & Miller G Epstein-Barr Virus-Induced Nodules on Viral Replication Compartments Contain RNA Processing Proteins and a Viral Long Noncoding RNA. J Virol 92:N/A (2018). Read more (PubMed: 30068640) »
