Anti-SON/DBP5 antibody (ab121759)
Key features and details
- Rabbit polyclonal to SON/DBP5
- Suitable for: ICC, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SON/DBP5 antibody -
Description
Rabbit polyclonal to SON/DBP5 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P Human
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Immunogen
Recombinant fragment corresponding to Human SON/DBP5 aa 1463-1598.
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Positive control
- IHC-P: Human small intestine, tonsil, prostate, and cerebellum tissue. ICC: U-251 MG cell lysates
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General notes
This product was previously labelled as SON
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC |
Human
|
| IHC-P |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use a concentration of 0.25 - 2 µg/ml.
|
|
| IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
ICC
Use a concentration of 0.25 - 2 µg/ml. |
|
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Represses hepatitis B virus (HBV) core promoter activity and transcription of HBV genes and production of HBV virions. Binds to the consensus DNA sequence: 5'-GA[GT]AN[CG][AG]CC-3'. Might protect cells from apoptosis. Might be involved in pre-mRNA splicing. -
Tissue specificity
Widely expressed, with the higher expression seen in leukocyte and heart. -
Sequence similarities
Contains 1 DRBM (double-stranded RNA-binding) domain.
Contains 1 G-patch domain. -
Domain
Contains 8 types of repeats which are distributed in 3 regions. -
Cellular localization
Nucleus speckle. Colocalizes with the pre-mRNA splicing factor SFRS2/SC-35. - Information by UniProt
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Database links
- Entrez Gene: 6651 Human
- Omim: 182465 Human
- SwissProt: P18583 Human
- Unigene: 517262 Human
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Alternative names
- BASS1 antibody
- Bax antagonist selected in saccharomyces 1 antibody
- DBP-5 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (ab121759)
Paraffin-embedded human small intestine tissue staining SON/DBP5 with ab121759 at 1/200 dilution in immunohistochemical analysis. Moderate to strong postiivty in nuclear speckles in glandular cells.
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Immunocytochemistry - Anti-SON/DBP5 antibody (ab121759)ab121759, at 4 µg/ml, staining SON/DBP5 (green) in PFA-fixed, Triton X-100 permbabilized U-251 MG cells, by Immunofluorescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (ab121759)Paraffin-embedded human prostate tissue staining SON/DBP5 with ab121759 at 1/200 dilution in immunohistochemical analysis. Moderate to strong positivity in nuclear speckles in glandular cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (ab121759)Paraffin-embedded human tonsil tissue staining SON/DBP5 with ab121759 at 1/200 dilution in immunohistochemical analysis. Moderate to strong positivity in nuclear speckles in germinal center cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody (ab121759)Paraffin-embedded human cerebellum tissue staining SON/DBP5 with ab121759 at 1/200 dilution in immunohistochemical analysis. Moderate to strong positivity in nuclear speckles in Purkinje cells.
Protocols
Datasheets and documents
References (3)
ab121759 has been referenced in 3 publications.
- Fáberová V et al. Super-Resolution Localisation of Nuclear PI(4)P and Identification of Its Interacting Proteome. Cells 9:N/A (2020). PubMed: 32403279
- Kim JH et al. SON haploinsufficiency causes impaired pre-mRNA splicing of CAKUT genes and heterogeneous renal phenotypes. Kidney Int 95:1494-1504 (2019). PubMed: 31005274
- Park R & Miller G Epstein-Barr Virus-Induced Nodules on Viral Replication Compartments Contain RNA Processing Proteins and a Viral Long Noncoding RNA. J Virol 92:N/A (2018). PubMed: 30068640
